Restriction map for
pF1KSDA0186
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HaeIII Hin1I HincII HindII
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AhdI 560 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 560 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 554 TGATCA T^GATCA
BglI 48 GCCCGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 355 AGATCT A^GATCT
BsaJI 17 CCATGG C^C[ACGT][ACGT]GG
BsaWI 449 TCCGGT [AT]^CCGG[AT]
BsgI 222 CTGCAC GTGCAG(16/14)
BssHII 44 GCGCGC G^CGCGC
BssKI 570 CCTGG ^CC[ACGT]GG
BstNI 570 CCTGG CC^[AT]GG
BstYI 355 AGATCT [GA]^GATC[CT]
DsaI 17 CCATGG C^C[GA][CT]GG
Eam1105I 560 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 570 CCTGG ^CC[AT]GG
EcoT14I 17 CCATGG C^C[AT][AT]GG
EcoT22I 299 ATGCAT ATGCA^T
FbaI 554 TGATCA T^GATCA
HapII 450 CCGG C^CGG
HindIII 110 AAGCTT A^AGCTT
HpaII 450 CCGG C^CGG
MaeIII 378 GTCAC ^GT[ACGT]AC
MboII 325 GAAGA GAAGA(8/7)
MflI 355 AGATCT [GA]^GATC[CT]
MslI 266 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 550 CAGCTG C[AC]G^C[GT]G
MspI 450 CCGG C^CGG
MvaI 570 CCTGG CC^[AT]GG
NcoI 17 CCATGG C^CATGG
NsiI 299 ATGCAT ATGCA^T
PvuII 550 CAGCTG CAG^CTG
ScrFI 570 CCTGG CC^[ACGT]GG
SfaNI 39 GCATC GCATC(5/9)
SfcI 227 CTGTAG C^T[GA][CT]AG
StyI 17 CCATGG C^C[AT][AT]GG
Tsp45I 378 GTCAC ^GT[GC]AC
XhoII 355 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 30 CCGC CCGC(-3/-1)
246 CCGC
AcsI 187 AAATTT [GA]^AATT[CT]
472 GAATTT
AlwI 27 GATCC GGATC(4/5)
257 GGATC
ApoI 187 AAATTT [GA]^AATT[CT]
472 GAATTT
AspHI 263 GAGCAC G[AT]GC[AT]^C
574 GAGCAC
BmyI 263 GAGCAC G[AGT]GC[ACT]^C
574 GAGCAC
BpmI 97 CTGGAG CTGGAG(16/14)
571 CTGGAG
BseRI 100 GAGGAG GAGGAG(10/8)
386 GAGGAG
BsiHKAI 263 GAGCAC G[AT]GC[AT]^C
574 GAGCAC
Bsp1286I 263 GAGCAC G[AGT]GC[ACT]^C
574 GAGCAC
BsrI 381 ACTGG ACTGG(1/-1)
129 CCAGT
BstF5I 579 CATCC GGATG(2/0)
78 GGATG
CfoI 44 GCGC GCG^C
46 GCGC
DdeI 85 CTCAG C^T[ACGT]AG
268 CTCAG
FokI 579 CATCC GGATG(9/13)
78 GGATG
HgaI 218 GACGC GACGC(5/10)
287 GCGTC
HhaI 44 GCGC GCG^C
46 GCGC
HinP1I 44 GCGC G^CGC
46 GCGC
HinfI 83 GACTC G^A[ACGT]TC
566 GAGTC
MwoI 39 GCATCGCGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
48 GCCCGAAGGGC
NlaIII 18 CATG CATG^
315 CATG
PleI 83 GACTC GAGTC(4/5)
566 GAGTC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AccII AluI BbvI BstUI Cac8I
DpnII Fnu4HI ItaI MboI MnlI
MseI MvnI NdeII Sau3AI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I