Restriction map for
pF1KSDA0174
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BcgI BciVI BglI BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HpaI
HphI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 300 GTCTAC GT^[AC][GT]AC
AcsI 39 GAATTT [GA]^AATT[CT]
AflII 30 CTTAAG C^TTAAG
AlwNI 1028 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 26 AGCGCT AGC^GCT
ApoI 39 GAATTT [GA]^AATT[CT]
AseI 856 ATTAAT AT^TAAT
AsnI 856 ATTAAT AT^TAAT
BamHI 158 GGATCC G^GATCC
BbvCI 1025 CCTCAGC CCTCAGC(-5/-2)
BclI 363 TGATCA T^GATCA
BfaI 274 CTAG C^TAG
BfrI 30 CTTAAG C^TTAAG
BglII 579 AGATCT A^GATCT
BlpI 22 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 22 GCTGAGC GC^T[ACGT]AGC
BsgI 881 CTGCAC GTGCAG(16/14)
BsmAI 574 GAGAC GTCTC(1/5)
BssSI 193 CTCGTG CACGAG(-5/-1)
BstF5I 805 CATCC GGATG(2/0)
BstXI 473 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 22 GCTGAGC GC^T[ACGT]AGC
CfoI 27 GCGC GCG^C
DraI 16 TTTAAA TTT^AAA
DraII 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 203 CCATGG C^C[GA][CT]GG
Eco47III 26 AGCGCT AGC^GCT
EcoO109I 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 203 CCATGG C^C[AT][AT]GG
FbaI 363 TGATCA T^GATCA
FokI 805 CATCC GGATG(9/13)
HaeII 26 AGCGCT [GA]GCGC^[CT]
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
HindIII 925 AAGCTT A^AGCTT
MaeI 274 CTAG C^TAG
MaeII 521 ACGT A^CGT
MaeIII 227 GTGAC ^GT[ACGT]AC
NcoI 203 CCATGG C^CATGG
PflMI 658 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 326 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 856 ATTAAT AT^TAAT
PstI 941 CTGCAG CTGCA^G
SacI 152 GAGCTC GAGCT^C
SapI 1072 GAAGAGC GCTCTTC(1/4)
SmlI 30 CTTAAG C^T[CT][GA]AG
SspI 799 AATATT AAT^ATT
StuI 788 AGGCCT AGG^CCT
StyI 203 CCATGG C^C[AT][AT]GG
TaqI 324 TCGA T^CGA
Tsp45I 227 GTGAC ^GT[GC]AC
TspRI 737 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 324 TCGA T^CGA
Van91I 658 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 222 GTAC GT^AC
523 GTAC
AspHI 152 GAGCTC G[AT]GC[AT]^C
169 GAGCAC
BanI 681 GGTGCC G^G[CT][GA]CC
1015 GGTGCC
BanII 6 GGGCTC G[GA]GC[CT]^C
152 GAGCTC
BbsI 184 GAAGAC GAAGAC(2/6)
1036 GAAGAC
BcnI 180 CCGGG CC^[GC]GG
1061 CCCGG
BpmI 328 CTCCAG CTGGAG(16/14)
214 CTGGAG
BpuAI 184 GAAGAC GAAGAC(2/6)
1036 GAAGAC
BsiHKAI 152 GAGCTC G[AT]GC[AT]^C
169 GAGCAC
BsmFI 747 GGGAC GGGAC(10/14)
778 GGGAC
BspMI 230 ACCTGC ACCTGC(4/8)
939 ACCTGC
DrdI 538 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
955 GACAACTTTGTC
EcoNI 192 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
625 CCTGGAAGAGG
HapII 180 CCGG C^CGG
1062 CCGG
HpaII 180 CCGG C^CGG
1062 CCGG
MslI 693 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
699 CATGCCCATG
MspI 180 CCGG C^CGG
1062 CCGG
MwoI 97 GCCCAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
978 GCCATCTGTGC
NciI 180 CCGGG CC^[GC]GG
1061 CCCGG
PleI 326 GACTC GAGTC(4/5)
538 GACTC
RsaI 222 GTAC GT^AC
523 GTAC
SfaNI 1006 GCATC GCATC(5/9)
1030 GCATC
SfcI 845 CTGTAG C^T[GA][CT]AG
941 CTGCAG
XcmI 203 CCATGGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
473 CCAAAATCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AlwI AvaII BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I XhoII