Restriction map for
pF1KSDA0171
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MluI MluNI
MscI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 22 CGCG CG^CG
AccIII 656 TCCGGA T^CCGGA
AflIII 8 ACATGT A^C[GA][CT]GT
Asp700I 163 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1335 GAGCAC G[AT]GC[AT]^C
BglII 730 AGATCT A^GATCT
BlpI 1052 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1052 GCTCAGC GC^T[ACGT]AGC
BsaI 805 GAGACC GGTCTC(1/5)
BsaWI 656 TCCGGA [AT]^CCGG[AT]
BseAI 656 TCCGGA T^CCGGA
BsiHKAI 1335 GAGCAC G[AT]GC[AT]^C
BsmI 906 GAATGC GAATGC(1/-1)
BspEI 656 TCCGGA T^CCGGA
BspLU11I 8 ACATGT A^CATGT
BstEII 970 GGTGACC G^GT[ACGT]ACC
BstPI 970 GGTGACC G^GT[ACGT]ACC
BstUI 22 CGCG CG^CG
BstXI 1679 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 730 AGATCT [GA]^GATC[CT]
CelII 1052 GCTCAGC GC^T[ACGT]AGC
DsaI 1679 CCATGG C^C[GA][CT]GG
EcoO65I 970 GGTGACC G^GT[ACGT]ACC
EcoRI 745 GAATTC G^AATTC
HincII 1005 GTCAAC GT[CT]^[GA]AC
HindII 1005 GTCAAC GT[CT]^[GA]AC
MfeI 632 CAATTG C^AATTG
MflI 730 AGATCT [GA]^GATC[CT]
MroI 656 TCCGGA T^CCGGA
MunI 632 CAATTG C^AATTG
MvnI 22 CGCG CG^CG
NcoI 1679 CCATGG C^CATGG
SapI 439 GAAGAGC GCTCTTC(1/4)
SexAI 1470 ACCTGGT A^CC[AT]GGT
SspI 379 AATATT AAT^ATT
XhoII 730 AGATCT [GA]^GATC[CT]
XmnI 163 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 1757 CCGC CCGC(-3/-1)
1198 GCGG
AlwNI 1055 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1148 CAGGCCCTG
BanI 994 GGCACC G^G[CT][GA]CC
1675 GGCACC
BanII 535 GAGCCC G[GA]GC[CT]^C
1281 GGGCTC
BbsI 673 GAAGAC GAAGAC(2/6)
930 GTCTTC
BpmI 680 CTCCAG CTGGAG(16/14)
1122 CTGGAG
BpuAI 673 GAAGAC GAAGAC(2/6)
930 GTCTTC
BseRI 556 GAGGAG GAGGAG(10/8)
760 GAGGAG
BsiYI 1421 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1757 CCGCTGCTGGG
BslI 1421 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1757 CCGCTGCTGGG
CfoI 21 GCGC GCG^C
828 GCGC
HapII 657 CCGG C^CGG
1611 CCGG
HhaI 21 GCGC GCG^C
828 GCGC
HinP1I 21 GCGC G^CGC
828 GCGC
HpaII 657 CCGG C^CGG
1611 CCGG
MseI 205 TTAA T^TAA
938 TTAA
MspI 657 CCGG C^CGG
1611 CCGG
PpuMI 111 GGGACCT [GA]G^G[AT]CC[CT]
1638 GGGACCC
PvuII 1019 CAGCTG CAG^CTG
1055 CAGCTG
SphI 1655 GCATGC GCATG^C
1796 GCATGC
TfiI 506 GATTC G^A[AT]TC
1518 GATTC
Tru9I 205 TTAA T^TAA
938 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BfaI BmyI BsaJI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I