Restriction map for
pF1KSDA0168
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT14I EcoT22I
FbaI FseI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
ApaI 744 GGGCCC GGGCC^C
Asp700I 223 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 919 GTGCTC G[AT]GC[AT]^C
BanI 125 GGCACC G^G[CT][GA]CC
BcnI 129 CCGGG CC^[GC]GG
BsaAI 670 TACGTG [CT]AC^GT[GA]
BsaI 961 GAGACC GGTCTC(1/5)
BsiHKAI 919 GTGCTC G[AT]GC[AT]^C
BsmAI 961 GAGAC GTCTC(1/5)
BsmI 227 GCATTC GAATGC(1/-1)
BsrDI 458 GCAATG GCAATG(2/0)
BsrI 819 CCAGT ACTGG(1/-1)
BstEII 352 GGTGACC G^GT[ACGT]ACC
BstPI 352 GGTGACC G^GT[ACGT]ACC
BstXI 678 CCATACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 428 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 453 CCGTGG C^C[GA][CT]GG
EaeI 516 CGGCCA [CT]^GGCC[GA]
EcoNI 396 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 352 GGTGACC G^GT[ACGT]ACC
EcoRI 645 GAATTC G^AATTC
HindIII 852 AAGCTT A^AGCTT
MslI 679 CATACGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 581 GCATCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 188 GCCGGC GCC^GGC
NciI 129 CCGGG CC^[GC]GG
NgoMIV 188 GCCGGC G^CCGGC
PflMI 553 CCAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 376 GACTC GAGTC(4/5)
PpuMI 608 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 415 CAGCTG CAG^CTG
SbfI 396 CCTGCAGG CCTGCA^GG
SexAI 788 ACCAGGT A^CC[AT]GGT
Sse8387I 396 CCTGCAGG CCTGCA^GG
TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 553 CCAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 223 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 468 GACGCC G[GA]^CG[CT]C
494 GACGCC
AviII 422 TGCGCA TGC^GCA
905 TGCGCA
BbsI 81 GAAGAC GAAGAC(2/6)
139 GAAGAC
BbvI 618 GCTGC GCAGC(8/12)
621 GCTGC
BfaI 25 CTAG C^TAG
473 CTAG
BglII 323 AGATCT A^GATCT
758 AGATCT
BpuAI 81 GAAGAC GAAGAC(2/6)
139 GAAGAC
BsaHI 468 GACGCC G[GA]^CG[CT]C
494 GACGCC
BsmFI 21 GTCCC GGGAC(10/14)
28 GTCCC
BsrFI 188 GCCGGC [GA]^CCGG[CT]
840 GCCGGT
BstYI 323 AGATCT [GA]^GATC[CT]
758 AGATCT
Cfr10I 188 GCCGGC [GA]^CCGG[CT]
840 GCCGGT
DdeI 281 CTCAG C^T[ACGT]AG
327 CTCAG
FauI 342 CCCGC CCCGC(4/6)
720 CCCGC
FspI 422 TGCGCA TGC^GCA
905 TGCGCA
Hin1I 468 GACGCC G[GA]^CG[CT]C
494 GACGCC
MaeI 25 CTAG C^TAG
473 CTAG
MflI 323 AGATCT [GA]^GATC[CT]
758 AGATCT
MseI 635 TTAA T^TAA
848 TTAA
PstI 202 CTGCAG CTGCA^G
397 CTGCAG
Tru9I 635 TTAA T^TAA
848 TTAA
TseI 618 GCTGC G^C[AT]GC
621 GCTGC
XhoII 323 AGATCT [GA]^GATC[CT]
758 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BanII BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tsp45I Tsp509I TthHB8I