Restriction map for
pF1KSDA0158
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 383 GGTACC G^GTACC
AflII 478 CTTAAG C^TTAAG
Asp718I 383 GGTACC G^GTACC
AvaI 1069 CTCGGG C^[CT]CG[GA]G
AvaII 876 GGACC G^G[AT]CC
BamHI 721 GGATCC G^GATCC
BbrPI 1078 CACGTG CAC^GTG
BbvI 211 GCAGC GCAGC(8/12)
BfrI 478 CTTAAG C^TTAAG
BglII 959 AGATCT A^GATCT
BlpI 979 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 979 GCTGAGC GC^T[ACGT]AGC
BsaAI 1078 CACGTG [CT]AC^GT[GA]
BsmAI 906 GAGAC GTCTC(1/5)
BsoBI 1069 CTCGGG C^[CT]CG[GA]G
BspMI 386 ACCTGC ACCTGC(4/8)
BsrDI 540 CATTGC GCAATG(2/0)
BstEII 867 GGTGACC G^GT[ACGT]ACC
BstPI 867 GGTGACC G^GT[ACGT]ACC
CelII 979 GCTGAGC GC^T[ACGT]AGC
CfoI 1021 GCGC GCG^C
Cfr13I 876 GGACC G^G[ACGT]CC
DraI 670 TTTAAA TTT^AAA
DraII 875 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1075 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 265 GAAGAG CTCTTC(1/4)
EcoO109I 875 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 867 GGTGACC G^GT[ACGT]ACC
HaeIII 763 GGCC GG^CC
HgaI 319 GACGC GACGC(5/10)
HhaI 1021 GCGC GCG^C
HinP1I 1021 GCGC G^CGC
KpnI 383 GGTACC GGTAC^C
Ksp632I 265 GAAGAG CTCTTC(1/4)
MaeII 1079 ACGT A^CGT
MwoI 1046 GCGGGGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 697 GCTAGC G^CTAGC
PmaCI 1078 CACGTG CAC^GTG
PmlI 1078 CACGTG CAC^GTG
PpuMI 875 AGGACCT [GA]G^G[AT]CC[CT]
PstI 330 CTGCAG CTGCA^G
SacI 982 GAGCTC GAGCT^C
SapI 265 GAAGAGC GCTCTTC(1/4)
Sau96I 876 GGACC G^G[ACGT]CC
SfcI 330 CTGCAG C^T[GA][CT]AG
SphI 992 GCATGC GCATG^C
SspI 526 AATATT AAT^ATT
TaqI 151 TCGA T^CGA
TseI 211 GCAGC G^C[AT]GC
TthHB8I 151 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 186 GTAC GT^AC
384 GTAC
AspHI 805 GAGCAC G[AT]GC[AT]^C
982 GAGCTC
BanI 383 GGTACC G^G[CT][GA]CC
1073 GGCACC
BanII 982 GAGCTC G[GA]GC[CT]^C
1064 GGGCTC
BpmI 859 CTCCAG CTGGAG(16/14)
206 CTGGAG
BsiHKAI 805 GAGCAC G[AT]GC[AT]^C
982 GAGCTC
BsrBI 399 GAGCGG CCGCTC(-3/-3)
577 GAGCGG
FauI 1046 GCGGG CCCGC(4/6)
1061 GCGGG
MaeIII 317 GTGAC ^GT[ACGT]AC
868 GTGAC
MslI 114 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
843 CACCCACATG
NspI 848 ACATGC [GA]CATG^[CT]
992 GCATGC
RsaI 186 GTAC GT^AC
384 GTAC
Tsp45I 317 GTGAC ^GT[GC]AC
868 GTGAC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
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AciI AluI AlwI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeI MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII NlaIV PleI Sau3AI ScrFI
SfaNI SmlI TfiI Tru9I Tsp509I
TspRI XhoII