Restriction map for
pF1KSDA0157
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 63 CGCG CG^CG
AclI 108 AACGTT AA^CGTT
AcyI 6 GGCGTC G[GA]^CG[CT]C
AspHI 436 GTGCTC G[AT]GC[AT]^C
AspI 370 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 906 GGATCC G^GATCC
BanII 1011 GGGCTC G[GA]GC[CT]^C
BcgI 895 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1050 GTATCC GTATCC(6/5)
BpmI 1094 CTGGAG CTGGAG(16/14)
BsaHI 6 GGCGTC G[GA]^CG[CT]C
BsgI 671 GTGCAG GTGCAG(16/14)
BsiHKAI 436 GTGCTC G[AT]GC[AT]^C
BsmAI 1097 GAGAC GTCTC(1/5)
BspMI 327 GCAGGT ACCTGC(4/8)
BstAPI 664 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 63 CGCG CG^CG
BstYI 906 GGATCC [GA]^GATC[CT]
DraII 1214 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1196 CCATGG C^C[GA][CT]GG
EcoO109I 1214 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 471 GATATC GAT^ATC
EcoT14I 1196 CCATGG C^C[AT][AT]GG
FauI 350 CCCGC CCCGC(4/6)
HaeII 49 AGCGCC [GA]GCGC^[CT]
HaeIII 900 GGCC GG^CC
HgaI 7 GCGTC GACGC(5/10)
Hin1I 6 GGCGTC G[GA]^CG[CT]C
HindIII 819 AAGCTT A^AGCTT
MaeII 109 ACGT A^CGT
MaeIII 122 GTGAC ^GT[ACGT]AC
MflI 906 GGATCC [GA]^GATC[CT]
MvnI 63 CGCG CG^CG
NcoI 1196 CCATGG C^CATGG
PflFI 370 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 124 GACTC GAGTC(4/5)
PpuMI 1214 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 108 AACGTT AA^CGTT
ScaI 952 AGTACT AGT^ACT
SmlI 1001 CTCAAG C^T[CT][GA]AG
StuI 899 AGGCCT AGG^CCT
StyI 1196 CCATGG C^C[AT][AT]GG
Tsp45I 122 GTGAC ^GT[GC]AC
Tth111I 370 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1083 CCAAAGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 906 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 145 GAATTT [GA]^AATT[CT]
1134 AAATTT
AlwI 907 GATCC GGATC(4/5)
906 GGATC
ApoI 145 GAATTT [GA]^AATT[CT]
1134 AAATTT
BbsI 734 GAAGAC GAAGAC(2/6)
525 GTCTTC
BfaI 493 CTAG C^TAG
503 CTAG
BpuAI 734 GAAGAC GAAGAC(2/6)
525 GTCTTC
BsrI 84 ACTGG ACTGG(1/-1)
1034 CCAGT
BssKI 1054 CCTGG ^CC[ACGT]GG
1220 CCAGG
BstNI 1054 CCTGG CC^[AT]GG
1220 CCAGG
CfoI 50 GCGC GCG^C
294 GCGC
DpnII 256 GATC ^GATC
907 GATC
EcoRII 1054 CCTGG ^CC[AT]GG
1220 CCAGG
HapII 291 CCGG C^CGG
1040 CCGG
HhaI 50 GCGC GCG^C
294 GCGC
HinP1I 50 GCGC G^CGC
294 GCGC
HpaII 291 CCGG C^CGG
1040 CCGG
MaeI 493 CTAG C^TAG
503 CTAG
MboI 256 GATC ^GATC
907 GATC
MspA1I 866 CAGCTG C[AC]G^C[GT]G
1091 CAGCTG
MspI 291 CCGG C^CGG
1040 CCGG
MvaI 1054 CCTGG CC^[AT]GG
1220 CCAGG
NdeII 256 GATC ^GATC
907 GATC
PvuII 866 CAGCTG CAG^CTG
1091 CAGCTG
SapI 438 GCTCTTC GCTCTTC(1/4)
1013 GCTCTTC
Sau3AI 256 GATC ^GATC
907 GATC
ScrFI 1054 CCTGG CC^[ACGT]GG
1220 CCAGG
SfcI 318 CTACAG C^T[GA][CT]AG
1148 CTACAG
TaqI 996 TCGA T^CGA
1191 TCGA
TthHB8I 996 TCGA T^CGA
1191 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BstF5I Cac8I Cfr13I DdeI
EarI Fnu4HI FokI HinfI HphI
ItaI Ksp632I MboII MnlI MseI
MwoI NlaIII NlaIV RsaI Sau96I
SfaNI TfiI Tru9I TseI Tsp509I
TspRI