Restriction map for
pF1KSDA0152
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 388 GTCTAC GT^[AC][GT]AC
AcsI 376 AAATTT [GA]^AATT[CT]
AlwNI 286 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 277 CCTGAGG CC^T[ACGT]AGG
ApoI 376 AAATTT [GA]^AATT[CT]
BalI 435 TGGCCA TGG^CCA
BamHI 191 GGATCC G^GATCC
BanII 88 GGGCTC G[GA]GC[CT]^C
BbsI 841 GTCTTC GAAGAC(2/6)
BpuAI 841 GTCTTC GAAGAC(2/6)
BsaAI 361 TACGTG [CT]AC^GT[GA]
BsaI 319 GAGACC GGTCTC(1/5)
BsgI 760 GTGCAG GTGCAG(16/14)
BsmAI 319 GAGAC GTCTC(1/5)
BsrBI 304 GAGCGG CCGCTC(-3/-3)
BstXI 821 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 277 CCTGAGG CC^T[ACGT]AGG
DraIII 439 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 277 CCTGAGG CC^T[ACGT]AGG
EcoNI 211 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 460 GATATC GAT^ATC
EcoT14I 608 CCAAGG C^C[AT][AT]GG
HaeII 36 GGCGCT [GA]GCGC^[CT]
HgaI 140 GCGTC GACGC(5/10)
HincII 154 GTCAAC GT[CT]^[GA]AC
HindII 154 GTCAAC GT[CT]^[GA]AC
HindIII 658 AAGCTT A^AGCTT
HinfI 839 GAGTC G^A[ACGT]TC
HphI 447 GGTGA GGTGA(8/7)
MluNI 435 TGGCCA TGG^CCA
MscI 435 TGGCCA TGG^CCA
MslI 822 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 29 CCGCTG C[AC]G^C[GT]G
NspI 174 GCATGT [GA]CATG^[CT]
PflMI 438 CCACGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 839 GAGTC GAGTC(4/5)
SfaNI 515 GCATC GCATC(5/9)
SgrAI 68 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 85 CCCGGG CCC^GGG
StyI 608 CCAAGG C^C[AT][AT]GG
TspRI 641 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 438 CCACGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 85 CCCGGG C^CCGGG
XmaI 85 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 114 CGCG CG^CG
159 CGCG
Alw44I 184 GTGCAC G^TGCAC
617 GTGCAC
ApaLI 184 GTGCAC G^TGCAC
617 GTGCAC
AspHI 184 GTGCAC G[AT]GC[AT]^C
617 GTGCAC
AvaI 85 CCCGGG C^[CT]CG[GA]G
133 CCCGAG
BseRI 316 GAGGAG GAGGAG(10/8)
349 GAGGAG
BsiHKAI 184 GTGCAC G[AT]GC[AT]^C
617 GTGCAC
BsoBI 85 CCCGGG C^[CT]CG[GA]G
133 CCCGAG
BstUI 114 CGCG CG^CG
159 CGCG
BstYI 191 GGATCC [GA]^GATC[CT]
287 AGATCC
EarI 856 CTCTTC CTCTTC(1/4)
691 GAAGAG
Ksp632I 856 CTCTTC CTCTTC(1/4)
691 GAAGAG
MflI 191 GGATCC [GA]^GATC[CT]
287 AGATCC
MvnI 114 CGCG CG^CG
159 CGCG
PpuMI 81 GGGACCC [GA]G^G[AT]CC[CT]
206 AGGACCC
SfiI 99 GGCCGGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
220 GGCCGGGTGGGCC
Tsp509I 377 AATT ^AATT
501 AATT
XhoII 191 GGATCC [GA]^GATC[CT]
287 AGATCC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BglI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MboI MboII
MnlI MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI