Restriction map for
pF1KSDA0149
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AhdI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BciVI BclI BfrI BglII BlnI
BsaBI BseAI BseRI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
ClaI DraI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SbfI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AgeI 1979 ACCGGT A^CCGGT
Alw44I 708 GTGCAC G^TGCAC
Aor51HI 980 AGCGCT AGC^GCT
ApaLI 708 GTGCAC G^TGCAC
AspI 1105 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1455 CCTCAGC CCTCAGC(-5/-2)
BcgI 2069 CGATCTACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1744 CTAG C^TAG
BsaI 1767 GGTCTC GGTCTC(1/5)
BsaWI 1979 ACCGGT [AT]^CCGG[AT]
BsgI 775 GTGCAG GTGCAG(16/14)
CpoI 2327 CGGTCCG CG^G[AT]CCG
DraIII 769 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 980 AGCGCT AGC^GCT
HphI 1479 GGTGA GGTGA(8/7)
MaeI 1744 CTAG C^TAG
NcoI 1167 CCATGG C^CATGG
NspI 2075 ACATGC [GA]CATG^[CT]
PflFI 1105 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1979 ACCGGT A^CCGGT
RsrII 2327 CGGTCCG CG^G[AT]CCG
SapI 1299 GCTCTTC GCTCTTC(1/4)
ScaI 278 AGTACT AGT^ACT
SfaNI 227 GATGC GCATC(5/9)
SgrAI 1978 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1354 CTCAAG C^T[CT][GA]AG
StuI 950 AGGCCT AGG^CCT
Tth111I 1105 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 1033 GGTACC G^GTACC
1783 GGTACC
AocI 1699 CCTGAGG CC^T[ACGT]AGG
1879 CCTGAGG
Asp718I 1033 GGTACC G^GTACC
1783 GGTACC
AviII 124 TGCGCA TGC^GCA
781 TGCGCA
BamHI 2098 GGATCC G^GATCC
2406 GGATCC
BbeI 529 GGCGCC GGCGC^C
560 GGCGCC
BbrPI 471 CACGTG CAC^GTG
2017 CACGTG
BlpI 109 GCTGAGC GC^T[ACGT]AGC
2011 GCTGAGC
Bpu1102I 109 GCTGAGC GC^T[ACGT]AGC
2011 GCTGAGC
BsaAI 471 CACGTG [CT]AC^GT[GA]
2017 CACGTG
BsrBI 1834 CCGCTC CCGCTC(-3/-3)
2115 CCGCTC
BssHII 508 GCGCGC G^CGCGC
736 GCGCGC
BstYI 2098 GGATCC [GA]^GATC[CT]
2406 GGATCC
Bsu36I 1699 CCTGAGG CC^T[ACGT]AGG
1879 CCTGAGG
CelII 109 GCTGAGC GC^T[ACGT]AGC
2011 GCTGAGC
DrdI 48 GACTCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1498 GACCCGGAGGTC
EagI 615 CGGCCG C^GGCCG
631 CGGCCG
EclXI 615 CGGCCG C^GGCCG
631 CGGCCG
Eco52I 615 CGGCCG C^GGCCG
631 CGGCCG
Eco81I 1699 CCTGAGG CC^T[ACGT]AGG
1879 CCTGAGG
EcoT14I 1167 CCATGG C^C[AT][AT]GG
2165 CCAAGG
FspI 124 TGCGCA TGC^GCA
781 TGCGCA
KasI 529 GGCGCC G^GCGCC
560 GGCGCC
KpnI 1033 GGTACC GGTAC^C
1783 GGTACC
MflI 2098 GGATCC [GA]^GATC[CT]
2406 GGATCC
MslI 82 CACGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
787 CACAGCTGTG
NarI 529 GGCGCC GG^CGCC
560 GGCGCC
PmaCI 471 CACGTG CAC^GTG
2017 CACGTG
PmlI 471 CACGTG CAC^GTG
2017 CACGTG
SacI 1822 GAGCTC GAGCT^C
2049 GAGCTC
SfoI 529 GGCGCC GGC^GCC
560 GGCGCC
StyI 1167 CCATGG C^C[AT][AT]GG
2165 CCAAGG
TaqI 225 TCGA T^CGA
1529 TCGA
TthHB8I 225 TCGA T^CGA
1529 TCGA
XhoII 2098 GGATCC [GA]^GATC[CT]
2406 GGATCC
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI Ksp632I
KspI MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfcI
SmaI TfiI TseI Tsp45I TspRI
Van91I XcmI XmaCI XmaI