Restriction map for
pF1KSDA0140
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FokI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1198 CGCG CG^CG
AcyI 848 GGCGCC G[GA]^CG[CT]C
Aor51HI 458 AGCGCT AGC^GCT
ApaI 1202 GGGCCC GGGCC^C
BbeI 848 GGCGCC GGCGC^C
BclI 275 TGATCA T^GATCA
BlpI 599 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 599 GCTCAGC GC^T[ACGT]AGC
BsaHI 848 GGCGCC G[GA]^CG[CT]C
BsaI 149 GAGACC GGTCTC(1/5)
BsmI 205 GAATGC GAATGC(1/-1)
BsrDI 48 CATTGC GCAATG(2/0)
BstUI 1198 CGCG CG^CG
BstXI 561 CCACCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 599 GCTCAGC GC^T[ACGT]AGC
DpnII 276 GATC ^GATC
DraIII 646 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 458 AGCGCT AGC^GCT
EcoNI 1146 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 275 TGATCA T^GATCA
Hin1I 848 GGCGCC G[GA]^CG[CT]C
KasI 848 GGCGCC G^GCGCC
KspI 1197 CCGCGG CCGC^GG
MboI 276 GATC ^GATC
MvnI 1198 CGCG CG^CG
NarI 848 GGCGCC GG^CGCC
NdeII 276 GATC ^GATC
NspI 52 GCATGT [GA]CATG^[CT]
SacII 1197 CCGCGG CCGC^GG
Sau3AI 276 GATC ^GATC
SfoI 848 GGCGCC GGC^GCC
StuI 874 AGGCCT AGG^CCT
TaqI 1088 TCGA T^CGA
Tsp509I 126 AATT ^AATT
TthHB8I 1088 TCGA T^CGA
XbaI 882 TCTAGA T^CTAGA
XcmI 558 CCACCACCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AspHI 189 GAGCAC G[AT]GC[AT]^C
526 GTGCTC
AvaI 32 CCCGGG C^[CT]CG[GA]G
515 CCCGGG
BfaI 326 CTAG C^TAG
883 CTAG
BglI 892 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1031 GCCCAGGGGGC
BpmI 777 CTCCAG CTGGAG(16/14)
630 CTGGAG
BsgI 76 CTGCAC GTGCAG(16/14)
643 CTGCAC
BsiHKAI 189 GAGCAC G[AT]GC[AT]^C
526 GTGCTC
BsmBI 429 GAGACG CGTCTC(1/5)
771 GAGACG
BsoBI 32 CCCGGG C^[CT]CG[GA]G
515 CCCGGG
BsrFI 662 GCCGGC [GA]^CCGG[CT]
1176 GCCGGC
Cfr10I 662 GCCGGC [GA]^CCGG[CT]
1176 GCCGGC
DrdI 823 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1093 GACCACCTCGTC
EarI 170 CTCTTC CTCTTC(1/4)
1073 CTCTTC
EcoT14I 395 CCTTGG C^C[AT][AT]GG
585 CCAAGG
Ksp632I 170 CTCTTC CTCTTC(1/4)
1073 CTCTTC
MaeI 326 CTAG C^TAG
883 CTAG
MaeII 524 ACGT A^CGT
986 ACGT
MseI 818 TTAA T^TAA
839 TTAA
NaeI 662 GCCGGC GCC^GGC
1176 GCCGGC
NgoMIV 662 GCCGGC G^CCGGC
1176 GCCGGC
PshAI 955 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1056 GACCCCGGTC
PstI 673 CTGCAG CTGCA^G
1181 CTGCAG
PvuII 930 CAGCTG CAG^CTG
1137 CAGCTG
SfaNI 197 GCATC GCATC(5/9)
290 GCATC
SmaI 32 CCCGGG CCC^GGG
515 CCCGGG
StyI 395 CCTTGG C^C[AT][AT]GG
585 CCAAGG
Tru9I 818 TTAA T^TAA
839 TTAA
XmaCI 32 CCCGGG C^CCGGG
515 CCCGGG
XmaI 32 CCCGGG C^CCGGG
515 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PflMI PleI PpuMI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI Van91I