Restriction map for
pF1KSDA0139
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I ApaLI AscI Asp718I AviII
BalI BbeI BbrPI BcgI BciVI
BfrI BlpI Bpu1102I BsaAI BseAI
BsiEI Bsp1407I BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII CpoI DraI
DraIII EaeI EagI EclXI Eco52I
EcoO65I EcoRV FseI FspI HpaI
KasI KpnI KspI MluNI MroI
MscI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 954 GTCTAC GT^[AC][GT]AC
AclI 2105 AACGTT AA^CGTT
AflIII 565 ACGCGT A^C[GA][CT]GT
AhdI 3509 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2714 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2914 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2134 AGCGCT AGC^GCT
ApaI 3411 GGGCCC GGGCC^C
AseI 1111 ATTAAT AT^TAAT
AsnI 1111 ATTAAT AT^TAAT
AspEI 3509 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1690 GAGCAC G[AT]GC[AT]^C
AvrII 3724 CCTAGG C^CTAGG
BclI 1452 TGATCA T^GATCA
BglI 2958 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3861 AGATCT A^GATCT
BlnI 3724 CCTAGG C^CTAGG
BsaWI 1263 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1690 GAGCAC G[AT]GC[AT]^C
BsiWI 1003 CGTACG C^GTACG
BspDI 2366 ATCGAT AT^CGAT
BsrDI 1429 CATTGC GCAATG(2/0)
BssHII 2490 GCGCGC G^CGCGC
Bsu36I 2914 CCTGAGG CC^T[ACGT]AGG
ClaI 2366 ATCGAT AT^CGAT
DrdI 1569 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 442 CCATGG C^C[GA][CT]GG
Eam1105I 3509 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2134 AGCGCT AGC^GCT
Eco81I 2914 CCTGAGG CC^T[ACGT]AGG
EcoNI 3052 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1452 TGATCA T^GATCA
HincII 233 GTCAAC GT[CT]^[GA]AC
HindII 233 GTCAAC GT[CT]^[GA]AC
HindIII 539 AAGCTT A^AGCTT
MluI 565 ACGCGT A^CGCGT
MslI 3031 CATGCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 3 GCCGGC GCC^GGC
NcoI 442 CCATGG C^CATGG
NgoMIV 3 GCCGGC G^CCGGC
NspI 1538 GCATGC [GA]CATG^[CT]
PflMI 3112 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1111 ATTAAT AT^TAAT
Psp1406I 2105 AACGTT AA^CGTT
PstI 1318 CTGCAG CTGCA^G
ScaI 1584 AGTACT AGT^ACT
SexAI 3495 ACCTGGT A^CC[AT]GGT
SphI 1538 GCATGC GCATG^C
Van91I 3112 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 2813 GGCGTC G[GA]^CG[CT]C
3026 GGCGTC
AspI 2906 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3494 GACCTGGTC
AvaI 1502 CTCGAG C^[CT]CG[GA]G
3575 CTCGAG
BamHI 1700 GGATCC G^GATCC
1992 GGATCC
BanI 1945 GGTGCC G^G[CT][GA]CC
2689 GGCACC
BanII 2839 GAGCCC G[GA]GC[CT]^C
3411 GGGCCC
BbvCI 1819 GCTGAGG CCTCAGC(-5/-2)
3757 GCTGAGG
BsaHI 2813 GGCGTC G[GA]^CG[CT]C
3026 GGCGTC
BsgI 2297 CTGCAC GTGCAG(16/14)
3395 GTGCAG
BsoBI 1502 CTCGAG C^[CT]CG[GA]G
3575 CTCGAG
BspMI 1323 GCAGGT ACCTGC(4/8)
1440 GCAGGT
BstAPI 1275 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1533 GCAAAGCATGC
EcoRI 577 GAATTC G^AATTC
3380 GAATTC
EcoT22I 682 ATGCAT ATGCA^T
875 ATGCAT
Hin1I 2813 GGCGTC G[GA]^CG[CT]C
3026 GGCGTC
MfeI 1173 CAATTG C^AATTG
1721 CAATTG
MunI 1173 CAATTG C^AATTG
1721 CAATTG
NsiI 682 ATGCAT ATGCA^T
875 ATGCAT
PaeR7I 1502 CTCGAG C^TCGAG
3575 CTCGAG
PflFI 2906 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3494 GACCTGGTC
SfcI 1318 CTGCAG C^T[GA][CT]AG
2418 CTATAG
SspI 358 AATATT AAT^ATT
1756 AATATT
StuI 2986 AGGCCT AGG^CCT
4058 AGGCCT
Tth111I 2906 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3494 GACCTGGTC
XhoI 1502 CTCGAG C^TCGAG
3575 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PshAI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI