Restriction map for
pF1KSDA0135
##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AflII AscI AviII AvrII
BbeI BclI BfrI BglII BlnI
BsaBI BsiWI BsmI BspDI BspLU11I
BssHII BssSI BstAPI BstBI ClaI
CpoI DraI DrdI EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1228 GACGTC GACGT^C
Acc65I 916 GGTACC G^GTACC
AccI 2720 GTATAC GT^[AC][GT]AC
AccIII 1361 TCCGGA T^CCGGA
AcsI 2208 GAATTC [GA]^AATT[CT]
AcyI 1228 GACGTC G[GA]^CG[CT]C
AgeI 976 ACCGGT A^CCGGT
AhdI 2257 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 186 AGCGCT AGC^GCT
ApoI 2208 GAATTC [GA]^AATT[CT]
AseI 1315 ATTAAT AT^TAAT
AsnI 1315 ATTAAT AT^TAAT
Asp718I 916 GGTACC G^GTACC
AspEI 2257 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1468 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2571 CACGTG CAC^GTG
BbsI 196 GAAGAC GAAGAC(2/6)
BciVI 3370 GGATAC GTATCC(6/5)
BpuAI 196 GAAGAC GAAGAC(2/6)
BsaHI 1228 GACGTC G[GA]^CG[CT]C
BseAI 1361 TCCGGA T^CCGGA
BspEI 1361 TCCGGA T^CCGGA
BspHI 3692 TCATGA T^CATGA
BsrDI 1235 GCAATG GCAATG(2/0)
Bst1107I 2720 GTATAC GTA^TAC
BstEII 2610 GGTCACC G^GT[ACGT]ACC
BstPI 2610 GGTCACC G^GT[ACGT]ACC
BstZ17I 2720 GTATAC GTA^TAC
DraIII 1339 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3467 CGGCCG C^GGCCG
Eam1105I 2257 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3467 CGGCCG C^GGCCG
Eco47III 186 AGCGCT AGC^GCT
Eco52I 3467 CGGCCG C^GGCCG
EcoO65I 2610 GGTCACC G^GT[ACGT]ACC
EcoRI 2208 GAATTC G^AATTC
HgaI 3734 GACGC GACGC(5/10)
Hin1I 1228 GACGTC G[GA]^CG[CT]C
HincII 2413 GTTGAC GT[CT]^[GA]AC
HindII 2413 GTTGAC GT[CT]^[GA]AC
HindIII 449 AAGCTT A^AGCTT
KpnI 916 GGTACC GGTAC^C
KspI 301 CCGCGG CCGC^GG
MroI 1361 TCCGGA T^CCGGA
NspI 1688 GCATGT [GA]CATG^[CT]
PflFI 1468 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 976 ACCGGT A^CCGGT
PmaCI 2571 CACGTG CAC^GTG
PmlI 2571 CACGTG CAC^GTG
PshAI 717 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1315 ATTAAT AT^TAAT
RcaI 3692 TCATGA T^CATGA
SacII 301 CCGCGG CCGC^GG
SexAI 3674 ACCTGGT A^CC[AT]GGT
SgrAI 975 CACCGGTG C[GA]^CCGG[CT]G
SmaI 3964 CCCGGG CCC^GGG
SspI 241 AATATT AAT^ATT
StuI 1902 AGGCCT AGG^CCT
Tth111I 1468 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1926 TCTAGA T^CTAGA
XmaCI 3964 CCCGGG C^CCGGG
XmaI 3964 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 1495 GTGCAC G^TGCAC
3527 GTGCAC
AocI 344 CCTCAGG CC^T[ACGT]AGG
2563 CCTGAGG
ApaLI 1495 GTGCAC G^TGCAC
3527 GTGCAC
Asp700I 2041 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3621 GAACCCCTTC
AvaI 1349 CCCGAG C^[CT]CG[GA]G
3964 CCCGGG
BcgI 3519 CGAGTACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1900 GCAGGCCTCTCG
BglI 2846 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3008 GCCCGCTGGGC
BlpI 103 GCTGAGC GC^T[ACGT]AGC
2875 GCTGAGC
Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC
2875 GCTGAGC
BsaAI 787 TACGTG [CT]AC^GT[GA]
2571 CACGTG
BsmBI 2702 GAGACG CGTCTC(1/5)
3732 GAGACG
BsoBI 1349 CCCGAG C^[CT]CG[GA]G
3964 CCCGGG
Bsp1407I 247 TGTACA T^GTACA
3599 TGTACA
BsrFI 976 ACCGGT [GA]^CCGG[CT]
2401 ACCGGC
BsrGI 247 TGTACA T^GTACA
3599 TGTACA
Bsu36I 344 CCTCAGG CC^T[ACGT]AGG
2563 CCTGAGG
CelII 103 GCTGAGC GC^T[ACGT]AGC
2875 GCTGAGC
Cfr10I 976 ACCGGT [GA]^CCGG[CT]
2401 ACCGGC
Eco81I 344 CCTCAGG CC^T[ACGT]AGG
2563 CCTGAGG
EcoNI 543 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1453 CCTGCTCCAGG
MslI 594 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2619 CACGCCCGTG
PflMI 2915 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3832 CCAGAAAGTGG
SapI 902 GAAGAGC GCTCTTC(1/4)
1963 GAAGAGC
ScaI 2981 AGTACT AGT^ACT
3521 AGTACT
SspBI 247 TGTACA T^GTACA
3599 TGTACA
Van91I 2915 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3832 CCAGAAAGTGG
XmnI 2041 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3621 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII