Restriction map for
pF1KSDA0127
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccIII AclI AfaI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 696 GACGTC GACGT^C
AccII 643 CGCG CG^CG
AcsI 681 GAATTT [GA]^AATT[CT]
AocI 243 CCTGAGG CC^T[ACGT]AGG
ApoI 681 GAATTT [GA]^AATT[CT]
AspHI 532 GAGCTC G[AT]GC[AT]^C
AvaI 406 CTCGAG C^[CT]CG[GA]G
BamHI 212 GGATCC G^GATCC
BanI 610 GGCACC G^G[CT][GA]CC
BcnI 393 CCCGG CC^[GC]GG
BglI 307 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 780 CTGCAC GTGCAG(16/14)
BsiHKAI 532 GAGCTC G[AT]GC[AT]^C
BsmAI 66 GTCTC GTCTC(1/5)
BsoBI 406 CTCGAG C^[CT]CG[GA]G
BspLU11I 200 ACATGT A^CATGT
BsrFI 312 GCCGGC [GA]^CCGG[CT]
BstUI 643 CGCG CG^CG
BstYI 212 GGATCC [GA]^GATC[CT]
Bsu36I 243 CCTGAGG CC^T[ACGT]AGG
Cfr10I 312 GCCGGC [GA]^CCGG[CT]
DdeI 244 CTGAG C^T[ACGT]AG
EaeI 563 CGGCCA [CT]^GGCC[GA]
Eco81I 243 CCTGAGG CC^T[ACGT]AGG
FauI 309 CCCGC CCCGC(4/6)
HaeII 107 AGCGCC [GA]GCGC^[CT]
HgaI 331 GCGTC GACGC(5/10)
MflI 212 GGATCC [GA]^GATC[CT]
MvnI 643 CGCG CG^CG
NaeI 312 GCCGGC GCC^GGC
NciI 393 CCCGG CC^[GC]GG
NgoMIV 312 GCCGGC G^CCGGC
NspI 200 ACATGT [GA]CATG^[CT]
PacI 190 TTAATTAA TTAAT^TAA
PaeR7I 406 CTCGAG C^TCGAG
PflMI 915 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 592 GGGACCT [GA]G^G[AT]CC[CT]
SacI 532 GAGCTC GAGCT^C
SfiI 306 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 380 AGGCCT AGG^CCT
Van91I 915 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 406 CTCGAG C^TCGAG
XhoII 212 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 330 GGCGTC G[GA]^CG[CT]C
696 GACGTC
AflIII 200 ACATGT A^C[GA][CT]GT
422 ACACGT
AlwI 213 GATCC GGATC(4/5)
212 GGATC
BsaHI 330 GGCGTC G[GA]^CG[CT]C
696 GACGTC
CfoI 108 GCGC GCG^C
644 GCGC
DpnII 213 GATC ^GATC
525 GATC
EcoT14I 86 CCAAGG C^C[AT][AT]GG
515 CCTTGG
HapII 313 CCGG C^CGG
394 CCGG
HhaI 108 GCGC GCG^C
644 GCGC
Hin1I 330 GGCGTC G[GA]^CG[CT]C
696 GACGTC
HinP1I 108 GCGC G^CGC
644 GCGC
HinfI 652 GACTC G^A[ACGT]TC
667 GACTC
HpaII 313 CCGG C^CGG
394 CCGG
MboI 213 GATC ^GATC
525 GATC
MboII 40 GAAGA GAAGA(8/7)
119 TCTTC
MseI 190 TTAA T^TAA
194 TTAA
MslI 37 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
921 CATGGAGGTG
MspI 313 CCGG C^CGG
394 CCGG
NdeII 213 GATC ^GATC
525 GATC
PleI 652 GACTC GAGTC(4/5)
667 GACTC
Sau3AI 213 GATC ^GATC
525 GATC
SmlI 406 CTCGAG C^T[CT][GA]AG
632 CTCAAG
StyI 86 CCAAGG C^C[AT][AT]GG
515 CCTTGG
Tru9I 190 TTAA T^TAA
194 TTAA
Tsp509I 192 AATT ^AATT
682 AATT
TspRI 401 CACTG CA[GC]TG[ACGT][ACGT]^
582 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeII MaeIII MnlI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TaqI TseI Tsp45I TthHB8I