Restriction map for
pF1KSDA0126
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BfrI BglI BlnI BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2584 GGTACC G^GTACC
AlwNI 1168 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2046 CCTGAGG CC^T[ACGT]AGG
Asp718I 2584 GGTACC G^GTACC
BalI 1814 TGGCCA TGG^CCA
BamHI 3000 GGATCC G^GATCC
BbsI 999 GAAGAC GAAGAC(2/6)
BclI 1599 TGATCA T^GATCA
BcnI 1048 CCGGG CC^[GC]GG
BpuAI 999 GAAGAC GAAGAC(2/6)
BsaI 2219 GAGACC GGTCTC(1/5)
BsaWI 2132 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1458 TGTACA T^GTACA
BspHI 824 TCATGA T^CATGA
BspLU11I 2282 ACATGT A^CATGT
BsrBI 207 CCGCTC CCGCTC(-3/-3)
BsrGI 1458 TGTACA T^GTACA
BstAPI 1629 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2046 CCTGAGG CC^T[ACGT]AGG
CfoI 2646 GCGC GCG^C
Eco81I 2046 CCTGAGG CC^T[ACGT]AGG
EcoRI 178 GAATTC G^AATTC
EcoRV 2761 GATATC GAT^ATC
EcoT22I 893 ATGCAT ATGCA^T
FbaI 1599 TGATCA T^GATCA
HhaI 2646 GCGC GCG^C
HinP1I 2646 GCGC G^CGC
HincII 661 GTTGAC GT[CT]^[GA]AC
HindII 661 GTTGAC GT[CT]^[GA]AC
KpnI 2584 GGTACC GGTAC^C
MluNI 1814 TGGCCA TGG^CCA
MscI 1814 TGGCCA TGG^CCA
NciI 1048 CCGGG CC^[GC]GG
NheI 133 GCTAGC G^CTAGC
NsiI 893 ATGCAT ATGCA^T
PacI 451 TTAATTAA TTAAT^TAA
PflMI 1262 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 971 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1636 CTGCAG CTGCA^G
RcaI 824 TCATGA T^CATGA
SacI 871 GAGCTC GAGCT^C
SexAI 2681 ACCTGGT A^CC[AT]GGT
SphI 1242 GCATGC GCATG^C
SspBI 1458 TGTACA T^GTACA
Van91I 1262 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 867 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 2139 ACGTGT A^C[GA][CT]GT
2282 ACATGT
Asp700I 955 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2664 GAACTACTTC
AspHI 871 GAGCTC G[AT]GC[AT]^C
3012 GAGCAC
AvaII 972 GGACC G^G[AT]CC
3135 GGACC
BanI 2584 GGTACC G^G[CT][GA]CC
2701 GGTGCC
BciVI 2116 GTATCC GTATCC(6/5)
2265 GTATCC
BlpI 1657 GCTCAGC GC^T[ACGT]AGC
2641 GCTGAGC
Bpu1102I 1657 GCTCAGC GC^T[ACGT]AGC
2641 GCTGAGC
BsaBI 1713 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2296 GATACCTATC
BseRI 1390 CTCCTC GAGGAG(10/8)
2794 GAGGAG
BsiHKAI 871 GAGCTC G[AT]GC[AT]^C
3012 GAGCAC
CelII 1657 GCTCAGC GC^T[ACGT]AGC
2641 GCTGAGC
DraII 407 AGGCCCT [GA]G^G[ACGT]CC[CT]
971 AGGACCC
DsaI 1790 CCATGG C^C[GA][CT]GG
3131 CCATGG
EaeI 1814 TGGCCA [CT]^GGCC[GA]
3145 CGGCCA
EcoO109I 407 AGGCCCT [GA]G^G[ACGT]CC[CT]
971 AGGACCC
MamI 1713 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2296 GATACCTATC
NcoI 1790 CCATGG C^CATGG
3131 CCATGG
SmlI 30 CTCAAG C^T[CT][GA]AG
1420 CTCAAG
XcmI 2405 CCATCTTCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3050 CCAGAGTCACTGTGG
XmnI 955 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2664 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BfaI BglII
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII