Restriction map for
pF1KSDA0119
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AspI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 64 GGCACC G^G[CT][GA]CC
BbsI 229 GAAGAC GAAGAC(2/6)
BcnI 696 CCCGG CC^[GC]GG
BpuAI 229 GAAGAC GAAGAC(2/6)
BsaWI 593 ACCGGA [AT]^CCGG[AT]
BseRI 172 GAGGAG GAGGAG(10/8)
BsgI 841 GTGCAG GTGCAG(16/14)
BsiYI 49 CCTGTATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 49 CCTGTATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 449 CCATGG C^C[GA][CT]GG
EarI 222 GAAGAG CTCTTC(1/4)
EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT]
Ksp632I 222 GAAGAG CTCTTC(1/4)
MaeII 839 ACGT A^CGT
MaeIII 109 GTCAC ^GT[ACGT]AC
MfeI 319 CAATTG C^AATTG
MseI 917 TTAA T^TAA
MslI 486 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 319 CAATTG C^AATTG
NciI 696 CCCGG CC^[GC]GG
NcoI 449 CCATGG C^CATGG
NheI 934 GCTAGC G^CTAGC
PflFI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 542 GACTC GAGTC(4/5)
PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT]
SexAI 566 ACCAGGT A^CC[AT]GGT
SmlI 544 CTCAAG C^T[CT][GA]AG
SspI 411 AATATT AAT^ATT
Tru9I 917 TTAA T^TAA
Tsp45I 109 GTCAC ^GT[GC]AC
Tth111I 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 777 CCAGCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 138 CCGC CCGC(-3/-1)
507 CCGC
AfaI 434 GTAC GT^AC
549 GTAC
AspHI 650 GTGCTC G[AT]GC[AT]^C
700 GTGCTC
BmyI 650 GTGCTC G[AGT]GC[ACT]^C
700 GTGCTC
BpmI 77 CTCCAG CTGGAG(16/14)
517 CTGGAG
BsiHKAI 650 GTGCTC G[AT]GC[AT]^C
700 GTGCTC
BsmAI 668 GAGAC GTCTC(1/5)
360 GTCTC
Bsp1286I 650 GTGCTC G[AGT]GC[ACT]^C
700 GTGCTC
BspMI 177 GCAGGT ACCTGC(4/8)
843 GCAGGT
BstYI 426 GGATCT [GA]^GATC[CT]
658 GGATCT
Cac8I 509 GCAGGC GC[ACGT]^[ACGT]GC
934 GCTAGC
CfoI 771 GCGC GCG^C
825 GCGC
HapII 594 CCGG C^CGG
697 CCGG
HhaI 771 GCGC GCG^C
825 GCGC
HinP1I 771 GCGC G^CGC
825 GCGC
HincII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindIII 98 AAGCTT A^AGCTT
250 AAGCTT
HpaII 594 CCGG C^CGG
697 CCGG
HphI 373 GGTGA GGTGA(8/7)
385 TCACC
MboII 222 GAAGA GAAGA(8/7)
229 GAAGA
MflI 426 GGATCT [GA]^GATC[CT]
658 GGATCT
MspA1I 514 CAGCTG C[AC]G^C[GT]G
778 CAGCTG
MspI 594 CCGG C^CGG
697 CCGG
PstI 781 CTGCAG CTGCA^G
866 CTGCAG
PvuII 514 CAGCTG CAG^CTG
778 CAGCTG
RsaI 434 GTAC GT^AC
549 GTAC
SfaNI 466 GATGC GCATC(5/9)
827 GCATC
TaqI 270 TCGA T^CGA
622 TCGA
TfiI 4 GATTC G^A[AT]TC
148 GATTC
TthHB8I 270 TCGA T^CGA
622 TCGA
XhoII 426 GGATCT [GA]^GATC[CT]
658 GGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AlwI AvaII BalI BbvI
BfaI BsaJI BsrI BssKI BstF5I
BstNI Cfr13I DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MboI MluNI MnlI
MscI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfcI
StyI TseI Tsp509I TspRI