Restriction map for
pF1KSDA0111
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BamHI BbvCI
BcgI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII BstAPI BstBI BstEII BstPI
CelII Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I FbaI
FseI FspI HgaI HincII HindII
HpaI KspI MaeI MamI MfeI
MluI MroI MspA1I MunI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
###################################
Acc65I 617 GGTACC G^GTACC
AccI 610 GTATAC GT^[AC][GT]AC
AclI 1216 AACGTT AA^CGTT
AcyI 976 GGCGCC G[GA]^CG[CT]C
AflIII 532 ACACGT A^C[GA][CT]GT
AlwI 363 GATCC GGATC(4/5)
Asp700I 960 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 617 GGTACC G^GTACC
AvaI 27 CTCGGG C^[CT]CG[GA]G
AvaII 141 GGACC G^G[AT]CC
BalI 1127 TGGCCA TGG^CCA
BanII 30 GGGCTC G[GA]GC[CT]^C
BbeI 976 GGCGCC GGCGC^C
BbrPI 533 CACGTG CAC^GTG
BbsI 61 GAAGAC GAAGAC(2/6)
BciVI 910 GTATCC GTATCC(6/5)
BglI 13 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 61 GAAGAC GAAGAC(2/6)
BsaAI 533 CACGTG [CT]AC^GT[GA]
BsaHI 976 GGCGCC G[GA]^CG[CT]C
BsaI 85 GAGACC GGTCTC(1/5)
BsaWI 968 TCCGGT [AT]^CCGG[AT]
BseRI 94 GAGGAG GAGGAG(10/8)
BsgI 358 GTGCAG GTGCAG(16/14)
BsoBI 27 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1079 TGTACA T^GTACA
BsrBI 943 GAGCGG CCGCTC(-3/-3)
BsrGI 1079 TGTACA T^GTACA
BssSI 667 CACGAG CACGAG(-5/-1)
Bst1107I 610 GTATAC GTA^TAC
BstYI 362 AGATCC [GA]^GATC[CT]
BstZ17I 610 GTATAC GTA^TAC
DraII 140 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 798 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1113 CGGCCG C^GGCCG
EarI 781 GAAGAG CTCTTC(1/4)
EclXI 1113 CGGCCG C^GGCCG
Eco52I 1113 CGGCCG C^GGCCG
EcoNI 132 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 140 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 79 GAATTC G^AATTC
EcoRV 1171 GATATC GAT^ATC
EcoT14I 125 CCATGG C^C[AT][AT]GG
EcoT22I 919 ATGCAT ATGCA^T
HaeII 976 GGCGCC [GA]GCGC^[CT]
Hin1I 976 GGCGCC G[GA]^CG[CT]C
HindIII 323 AAGCTT A^AGCTT
KasI 976 GGCGCC G^GCGCC
KpnI 617 GGTACC GGTAC^C
Ksp632I 781 GAAGAG CTCTTC(1/4)
MflI 362 AGATCC [GA]^GATC[CT]
MluNI 1127 TGGCCA TGG^CCA
MscI 1127 TGGCCA TGG^CCA
NarI 976 GGCGCC GG^CGCC
NcoI 125 CCATGG C^CATGG
NsiI 919 ATGCAT ATGCA^T
PflMI 291 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 533 CACGTG CAC^GTG
PmlI 533 CACGTG CAC^GTG
PpuMI 140 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1216 AACGTT AA^CGTT
ScaI 1181 AGTACT AGT^ACT
SfcI 998 CTACAG C^T[GA][CT]AG
SfoI 976 GGCGCC GGC^GCC
SmlI 320 CTCAAG C^T[CT][GA]AG
SspBI 1079 TGTACA T^GTACA
StyI 125 CCATGG C^C[AT][AT]GG
Van91I 291 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 362 AGATCC [GA]^GATC[CT]
XmnI 960 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 38 CGCG CG^CG
480 CGCG
AcsI 79 GAATTC [GA]^AATT[CT]
790 AAATTT
AocI 525 CCTAAGG CC^T[ACGT]AGG
1030 CCTCAGG
ApoI 79 GAATTC [GA]^AATT[CT]
790 AAATTT
BmyI 30 GGGCTC G[AGT]GC[ACT]^C
483 GGGCAC
BsiEI 970 CGGTCG CG[GA][CT]^CG
1113 CGGCCG
BsmFI 1027 GTCCC GGGAC(10/14)
1038 GTCCC
Bsp1286I 30 GGGCTC G[AGT]GC[ACT]^C
483 GGGCAC
BspHI 695 TCATGA T^CATGA
956 TCATGA
BspMI 143 ACCTGC ACCTGC(4/8)
620 ACCTGC
BssKI 490 CCAGG ^CC[ACGT]GG
1013 CCAGG
BstNI 490 CCAGG CC^[AT]GG
1013 CCAGG
BstUI 38 CGCG CG^CG
480 CGCG
BstXI 632 CCACACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1013 CCAGGGGGTTGG
Bsu36I 525 CCTAAGG CC^T[ACGT]AGG
1030 CCTCAGG
CfoI 37 GCGC GCG^C
977 GCGC
DsaI 8 CCACGG C^C[GA][CT]GG
125 CCATGG
Eco81I 525 CCTAAGG CC^T[ACGT]AGG
1030 CCTCAGG
EcoRII 490 CCAGG ^CC[AT]GG
1013 CCAGG
HhaI 37 GCGC GCG^C
977 GCGC
HinP1I 37 GCGC G^CGC
977 GCGC
HphI 388 GGTGA GGTGA(8/7)
720 GGTGA
MseI 1049 TTAA T^TAA
1133 TTAA
MslI 633 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
714 CATCTTGGTG
MvaI 490 CCAGG CC^[AT]GG
1013 CCAGG
MvnI 38 CGCG CG^CG
480 CGCG
NspI 471 GCATGT [GA]CATG^[CT]
929 ACATGC
PleI 738 GACTC GAGTC(4/5)
949 GAGTC
RcaI 695 TCATGA T^CATGA
956 TCATGA
ScrFI 490 CCAGG CC^[ACGT]GG
1013 CCAGG
TfiI 510 GATTC G^A[AT]TC
672 GATTC
Tru9I 1049 TTAA T^TAA
1133 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI BanI BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsrI BstF5I Cac8I Cfr13I DdeI
DpnII EaeI FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MspI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I SfaNI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI