Restriction map for
pF1KSDA0093
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 12 CGCG CG^CG
AvaI 2492 CTCGGG C^[CT]CG[GA]G
AvrII 306 CCTAGG C^CTAGG
BanI 2496 GGTGCC G^G[CT][GA]CC
BlnI 306 CCTAGG C^CTAGG
BsaAI 127 TACGTG [CT]AC^GT[GA]
BsaWI 1670 ACCGGA [AT]^CCGG[AT]
BsmI 903 GAATGC GAATGC(1/-1)
BsoBI 2492 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1140 TGTACA T^GTACA
BspHI 2013 TCATGA T^CATGA
BspMI 1230 GCAGGT ACCTGC(4/8)
BsrFI 254 ACCGGC [GA]^CCGG[CT]
BsrGI 1140 TGTACA T^GTACA
BstAPI 718 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1233 GGTGACC G^GT[ACGT]ACC
BstPI 1233 GGTGACC G^GT[ACGT]ACC
BstUI 12 CGCG CG^CG
BstXI 1051 CCACCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 11 GCGC GCG^C
Cfr10I 254 ACCGGC [GA]^CCGG[CT]
DraI 2254 TTTAAA TTT^AAA
DraIII 719 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1550 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1233 GGTGACC G^GT[ACGT]ACC
HhaI 11 GCGC GCG^C
HinP1I 11 GCGC G^CGC
HincII 769 GTTGAC GT[CT]^[GA]AC
HindII 769 GTTGAC GT[CT]^[GA]AC
MfeI 2666 CAATTG C^AATTG
MspA1I 1165 CAGCTG C[AC]G^C[GT]G
MunI 2666 CAATTG C^AATTG
MvnI 12 CGCG CG^CG
NspI 1290 GCATGC [GA]CATG^[CT]
PpuMI 616 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2537 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1187 CTGCAG CTGCA^G
PvuII 1165 CAGCTG CAG^CTG
RcaI 2013 TCATGA T^CATGA
SexAI 1053 ACCAGGT A^CC[AT]GGT
SmlI 1173 CTCAAG C^T[CT][GA]AG
SphI 1290 GCATGC GCATG^C
SspBI 1140 TGTACA T^GTACA
XbaI 640 TCTAGA T^CTAGA
XcmI 2679 CCAGGGCTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 1141 GTAC GT^AC
2567 GTAC
AlwNI 533 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
686 CAGATGCTG
AocI 670 CCTCAGG CC^T[ACGT]AGG
2091 CCTCAGG
AspHI 954 GAGCTC G[AT]GC[AT]^C
2588 GAGCTC
BbsI 1660 GAAGAC GAAGAC(2/6)
783 GTCTTC
BbvCI 244 CCTCAGC CCTCAGC(-5/-2)
493 GCTGAGG
BpuAI 1660 GAAGAC GAAGAC(2/6)
783 GTCTTC
BsaI 363 GAGACC GGTCTC(1/5)
1156 GAGACC
BseRI 572 CTCCTC GAGGAG(10/8)
1757 GAGGAG
BsiHKAI 954 GAGCTC G[AT]GC[AT]^C
2588 GAGCTC
Bsu36I 670 CCTCAGG CC^T[ACGT]AGG
2091 CCTCAGG
Eco81I 670 CCTCAGG CC^T[ACGT]AGG
2091 CCTCAGG
EcoRV 604 GATATC GAT^ATC
747 GATATC
EcoT14I 306 CCTAGG C^C[AT][AT]GG
609 CCTTGG
MslI 1450 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2131 CATCAACATG
NlaIV 29 GGCTCC GG[ACGT]^[ACGT]CC
2496 GGTGCC
RsaI 1141 GTAC GT^AC
2567 GTAC
SacI 954 GAGCTC GAGCT^C
2588 GAGCTC
StyI 306 CCTAGG C^C[AT][AT]GG
609 CCTTGG
TaqI 952 TCGA T^CGA
1713 TCGA
TthHB8I 952 TCGA T^CGA
1713 TCGA
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII PflMI PleI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StuI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII