Restriction map for
pF1KSDA0086
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BalI BamHI BanII BbeI
BbvCI BcnI BglII BlnI BlpI
Bpu1102I BsaI BseAI BseRI BsiEI
BsiWI Bsp1407I BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CpoI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoT22I FauI FseI
FspI HaeIII HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 2468 AACGTT AA^CGTT
AcyI 610 GGCGTC G[GA]^CG[CT]C
AflII 1624 CTTAAG C^TTAAG
Aor51HI 3002 AGCGCT AGC^GCT
AspHI 3072 GAGCAC G[AT]GC[AT]^C
AvaI 1100 CTCGGG C^[CT]CG[GA]G
BanI 3055 GGCACC G^G[CT][GA]CC
BbrPI 325 CACGTG CAC^GTG
BcgI 402 GCAGACACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 358 GGATAC GTATCC(6/5)
BfrI 1624 CTTAAG C^TTAAG
BglI 2584 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 290 CTCCAG CTGGAG(16/14)
BsaAI 325 CACGTG [CT]AC^GT[GA]
BsaBI 2368 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 610 GGCGTC G[GA]^CG[CT]C
BsgI 2772 GTGCAG GTGCAG(16/14)
BsiHKAI 3072 GAGCAC G[AT]GC[AT]^C
BsmBI 716 CGTCTC CGTCTC(1/5)
BsmI 1482 GAATGC GAATGC(1/-1)
BsoBI 1100 CTCGGG C^[CT]CG[GA]G
BspDI 934 ATCGAT AT^CGAT
BsrFI 2128 ACCGGC [GA]^CCGG[CT]
BstYI 2454 AGATCC [GA]^GATC[CT]
CfoI 3003 GCGC GCG^C
Cfr10I 2128 ACCGGC [GA]^CCGG[CT]
ClaI 934 ATCGAT AT^CGAT
DraII 1783 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 2485 GACCAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3002 AGCGCT AGC^GCT
EcoNI 574 CCTGCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1783 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 931 GATATC GAT^ATC
HaeII 3002 AGCGCT [GA]GCGC^[CT]
HhaI 3003 GCGC GCG^C
Hin1I 610 GGCGTC G[GA]^CG[CT]C
HinP1I 3003 GCGC G^CGC
MamI 2368 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2454 AGATCC [GA]^GATC[CT]
MslI 407 CACCTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1017 CAGCTG C[AC]G^C[GT]G
NdeI 2497 CATATG CA^TATG
NheI 528 GCTAGC G^CTAGC
PflMI 70 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 325 CACGTG CAC^GTG
PmlI 325 CACGTG CAC^GTG
PpuMI 1783 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2468 AACGTT AA^CGTT
PvuII 1017 CAGCTG CAG^CTG
SpeI 807 ACTAGT A^CTAGT
Van91I 70 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2454 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 161 CCGC CCGC(-3/-1)
2482 GCGG
AspI 600 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1040 GACATGGTC
BclI 549 TGATCA T^GATCA
2205 TGATCA
BsaWI 945 ACCGGA [AT]^CCGG[AT]
2564 TCCGGT
BspHI 927 TCATGA T^CATGA
2393 TCATGA
BspLU11I 2098 ACATGT A^CATGT
2768 ACATGT
Cac8I 528 GCTAGC GC[ACGT]^[ACGT]GC
1251 GCTTGC
EcoRI 240 GAATTC G^AATTC
2963 GAATTC
EcoT14I 198 CCTTGG C^C[AT][AT]GG
1652 CCAAGG
FbaI 549 TGATCA T^GATCA
2205 TGATCA
HindIII 1249 AAGCTT A^AGCTT
2284 AAGCTT
PflFI 600 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1040 GACATGGTC
PstI 1515 CTGCAG CTGCA^G
1930 CTGCAG
RcaI 927 TCATGA T^CATGA
2393 TCATGA
ScaI 1180 AGTACT AGT^ACT
1843 AGTACT
SmlI 625 CTTGAG C^T[CT][GA]AG
1624 CTTAAG
StyI 198 CCTTGG C^C[AT][AT]GG
1652 CCAAGG
Tsp45I 881 GTGAC ^GT[GC]AC
1890 GTCAC
Tth111I 600 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1040 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI