Restriction map for
pF1KSDA0085
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeII MaeIII MfeI MflI MluI
MroI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 607 GTCTAC GT^[AC][GT]AC
AcyI 141 GGCGTC G[GA]^CG[CT]C
AfaI 270 GTAC GT^AC
AflIII 611 ACATGT A^C[GA][CT]GT
AlwI 176 GGATC GGATC(4/5)
AlwNI 418 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 171 CCTCAGG CC^T[ACGT]AGG
ApaI 756 GGGCCC GGGCC^C
AspHI 112 GAGCAC G[AT]GC[AT]^C
BalI 71 TGGCCA TGG^CCA
BbsI 741 GAAGAC GAAGAC(2/6)
BfaI 386 CTAG C^TAG
BpuAI 741 GAAGAC GAAGAC(2/6)
BsaHI 141 GGCGTC G[GA]^CG[CT]C
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
BsmFI 288 GTCCC GGGAC(10/14)
BspHI 89 TCATGA T^CATGA
BspLU11I 611 ACATGT A^CATGT
BsrI 546 CCAGT ACTGG(1/-1)
BstF5I 441 CATCC GGATG(2/0)
BstXI 62 CCACCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 171 CCTCAGG CC^T[ACGT]AGG
DraII 755 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 132 CCATGG C^C[GA][CT]GG
EaeI 71 TGGCCA [CT]^GGCC[GA]
Eco81I 171 CCTCAGG CC^T[ACGT]AGG
EcoO109I 755 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 132 CCATGG C^C[AT][AT]GG
FokI 441 CATCC GGATG(9/13)
Hin1I 141 GGCGTC G[GA]^CG[CT]C
MaeI 386 CTAG C^TAG
MluNI 71 TGGCCA TGG^CCA
MscI 71 TGGCCA TGG^CCA
MspA1I 418 CAGCTG C[AC]G^C[GT]G
NcoI 132 CCATGG C^CATGG
NheI 385 GCTAGC G^CTAGC
NspI 611 ACATGT [GA]CATG^[CT]
PflMI 693 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 418 CAGCTG CAG^CTG
RcaI 89 TCATGA T^CATGA
RsaI 270 GTAC GT^AC
SapI 216 GCTCTTC GCTCTTC(1/4)
SmlI 361 CTCAAG C^T[CT][GA]AG
StyI 132 CCATGG C^C[AT][AT]GG
Tsp509I 511 AATT ^AATT
Van91I 693 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AvaII 3 GGACC G^G[AT]CC
702 GGTCC
BanII 333 GGGCTC G[GA]GC[CT]^C
756 GGGCCC
BcnI 261 CCGGG CC^[GC]GG
374 CCCGG
BpmI 192 CTCCAG CTGGAG(16/14)
691 CTCCAG
BsaBI 558 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
561 GATGATCATC
BsaJI 68 CCCTGG C^C[ACGT][ACGT]GG
132 CCATGG
BseRI 390 CTCCTC GAGGAG(10/8)
667 GAGGAG
BspMI 31 ACCTGC ACCTGC(4/8)
272 ACCTGC
BstNI 69 CCTGG CC^[AT]GG
525 CCAGG
EarI 217 CTCTTC CTCTTC(1/4)
672 GAAGAG
EcoRII 69 CCTGG ^CC[AT]GG
525 CCAGG
FauI 8 CCCGC CCCGC(4/6)
352 CCCGC
HapII 261 CCGG C^CGG
375 CCGG
HpaII 261 CCGG C^CGG
375 CCGG
HphI 209 TCACC GGTGA(8/7)
323 TCACC
Ksp632I 217 CTCTTC CTCTTC(1/4)
672 GAAGAG
MamI 558 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
561 GATGATCATC
MspI 261 CCGG C^CGG
375 CCGG
MvaI 69 CCTGG CC^[AT]GG
525 CCAGG
NciI 261 CCGGG CC^[GC]GG
374 CCCGG
PstI 415 CTGCAG CTGCA^G
453 CTGCAG
TaqI 110 TCGA T^CGA
739 TCGA
TspRI 18 CACTG CA[GC]TG[ACGT][ACGT]^
588 CACTG
TthHB8I 110 TCGA T^CGA
739 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BclI BmyI
BsiYI BslI Bsp1286I BssKI Cac8I
Cfr13I DdeI DpnII FbaI Fnu4HI
HaeIII HgaI ItaI MboI MboII
MnlI MslI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TseI