Restriction map for
pF1KSDA0080
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BsaAI BsaHI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT14I EcoT22I
FbaI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MseI MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1006 GTCTAC GT^[AC][GT]AC
Alw44I 1056 GTGCAC G^TGCAC
ApaLI 1056 GTGCAC G^TGCAC
AvaI 758 CTCGGG C^[CT]CG[GA]G
BalI 1265 TGGCCA TGG^CCA
BamHI 475 GGATCC G^GATCC
BanI 786 GGTGCC G^G[CT][GA]CC
BlpI 309 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 309 GCTAAGC GC^T[ACGT]AGC
BsaBI 960 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 457 GAGGAG GAGGAG(10/8)
BsgI 724 CTGCAC GTGCAG(16/14)
BsiEI 1152 CGATCG CG[GA][CT]^CG
BsmFI 836 GGGAC GGGAC(10/14)
BsoBI 758 CTCGGG C^[CT]CG[GA]G
BsrDI 1026 CATTGC GCAATG(2/0)
BstEII 93 GGTGACC G^GT[ACGT]ACC
BstPI 93 GGTGACC G^GT[ACGT]ACC
BstXI 368 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 309 GCTAAGC GC^T[ACGT]AGC
CfoI 1023 GCGC GCG^C
DraIII 1199 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 543 CCACGG C^C[GA][CT]GG
EcoO65I 93 GGTGACC G^GT[ACGT]ACC
FauI 1253 CCCGC CCCGC(4/6)
FseI 51 GGCCGGCC GGCCGG^CC
HgaI 289 GACGC GACGC(5/10)
HhaI 1023 GCGC GCG^C
HinP1I 1023 GCGC G^CGC
HinfI 1081 GAATC G^A[ACGT]TC
MamI 960 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1265 TGGCCA TGG^CCA
MscI 1265 TGGCCA TGG^CCA
MspA1I 706 CAGCTG C[AC]G^C[GT]G
NaeI 52 GCCGGC GCC^GGC
NgoMIV 52 GCCGGC G^CCGGC
NspI 170 ACATGC [GA]CATG^[CT]
PpuMI 713 AGGACCT [GA]G^G[AT]CC[CT]
PstI 709 CTGCAG CTGCA^G
PvuI 1152 CGATCG CGAT^CG
PvuII 706 CAGCTG CAG^CTG
SacI 880 GAGCTC GAGCT^C
SexAI 716 ACCTGGT A^CC[AT]GGT
SgrAI 41 CACCGGTG C[GA]^CCGG[CT]G
TfiI 1081 GAATC G^A[AT]TC
Tsp509I 362 AATT ^AATT
XcmI 1163 CCAAGAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 823 GTAC GT^AC
1290 GTAC
AgeI 42 ACCGGT A^CCGGT
970 ACCGGT
AlwNI 296 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
712 CAGGACCTG
AspHI 880 GAGCTC G[AT]GC[AT]^C
1056 GTGCAC
BbsI 630 GAAGAC GAAGAC(2/6)
648 GAAGAC
BbvCI 204 CCTCAGC CCTCAGC(-5/-2)
738 CCTCAGC
BpuAI 630 GAAGAC GAAGAC(2/6)
648 GAAGAC
BsaWI 42 ACCGGT [AT]^CCGG[AT]
970 ACCGGT
BsiHKAI 880 GAGCTC G[AT]GC[AT]^C
1056 GTGCAC
EaeI 50 TGGCCG [CT]^GGCC[GA]
1265 TGGCCA
EcoRV 964 GATATC GAT^ATC
1120 GATATC
MaeII 270 ACGT A^CGT
1004 ACGT
PinAI 42 ACCGGT A^CCGGT
970 ACCGGT
RsaI 823 GTAC GT^AC
1290 GTAC
SfcI 699 CTACAG C^T[GA][CT]AG
709 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BmyI BpmI BsaI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I XhoII