Restriction map for
pF1KSDA0070
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaAI BsaI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoRI EcoT22I FauI FseI FspI
HpaI KasI KpnI KspI MaeII
MfeI MluI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 965 TCCGGA T^CCGGA
AocI 196 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1451 AGCGCT AGC^GCT
AseI 514 ATTAAT AT^TAAT
AsnI 514 ATTAAT AT^TAAT
AspHI 124 GAGCTC G[AT]GC[AT]^C
BamHI 392 GGATCC G^GATCC
BanI 1424 GGCACC G^G[CT][GA]CC
BclI 1389 TGATCA T^GATCA
BcnI 939 CCGGG CC^[GC]GG
BfaI 790 CTAG C^TAG
BpmI 896 CTCCAG CTGGAG(16/14)
BsaBI 810 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 965 TCCGGA [AT]^CCGG[AT]
BseAI 965 TCCGGA T^CCGGA
BseRI 443 GAGGAG GAGGAG(10/8)
BsgI 10 GTGCAG GTGCAG(16/14)
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
BsmBI 72 GAGACG CGTCTC(1/5)
BsmI 1297 GAATGC GAATGC(1/-1)
BspEI 965 TCCGGA T^CCGGA
BspHI 1469 TCATGA T^CATGA
BsrDI 1487 GCAATG GCAATG(2/0)
Bst1107I 1494 GTATAC GTA^TAC
BstEII 1110 GGTCACC G^GT[ACGT]ACC
BstPI 1110 GGTCACC G^GT[ACGT]ACC
BstXI 1119 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 392 GGATCC [GA]^GATC[CT]
BstZ17I 1494 GTATAC GTA^TAC
Bsu36I 196 CCTGAGG CC^T[ACGT]AGG
CfoI 1452 GCGC GCG^C
DraII 192 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 833 CCGTGG C^C[GA][CT]GG
Eco47III 1451 AGCGCT AGC^GCT
Eco81I 196 CCTGAGG CC^T[ACGT]AGG
EcoO109I 192 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1110 GGTCACC G^GT[ACGT]ACC
EcoRV 1273 GATATC GAT^ATC
FbaI 1389 TGATCA T^GATCA
HaeII 1451 AGCGCT [GA]GCGC^[CT]
HhaI 1452 GCGC GCG^C
HinP1I 1452 GCGC G^CGC
HincII 1153 GTTGAC GT[CT]^[GA]AC
HindII 1153 GTTGAC GT[CT]^[GA]AC
HindIII 1336 AAGCTT A^AGCTT
MaeI 790 CTAG C^TAG
MamI 810 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 392 GGATCC [GA]^GATC[CT]
MroI 965 TCCGGA T^CCGGA
MslI 1120 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 939 CCGGG CC^[GC]GG
NdeI 631 CATATG CA^TATG
PpuMI 192 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 514 ATTAAT AT^TAAT
RcaI 1469 TCATGA T^CATGA
SacI 124 GAGCTC GAGCT^C
SapI 1192 GAAGAGC GCTCTTC(1/4)
ScaI 1698 AGTACT AGT^ACT
SfiI 1129 GGCCCAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1198 CTTGAG C^T[CT][GA]AG
XcmI 1751 CCACTGATACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 392 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccI 301 GTAGAC GT^[AC][GT]AC
1494 GTATAC
AcyI 74 GACGCC G[GA]^CG[CT]C
956 GACGCC
AfaI 1601 GTAC GT^AC
1699 GTAC
Asp700I 502 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1696 GAAGTACTTC
BalI 470 TGGCCA TGG^CCA
836 TGGCCA
BanII 124 GAGCTC G[GA]GC[CT]^C
1407 GAGCCC
BbsI 274 GAAGAC GAAGAC(2/6)
1726 GAAGAC
BglI 7 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1130 GCCCAGAGGGC
BlpI 141 GCTAAGC GC^T[ACGT]AGC
585 GCTGAGC
BmyI 124 GAGCTC G[AGT]GC[ACT]^C
1407 GAGCCC
Bpu1102I 141 GCTAAGC GC^T[ACGT]AGC
585 GCTGAGC
BpuAI 274 GAAGAC GAAGAC(2/6)
1726 GAAGAC
BsaHI 74 GACGCC G[GA]^CG[CT]C
956 GACGCC
Bsp1286I 124 GAGCTC G[AGT]GC[ACT]^C
1407 GAGCCC
BsrBI 738 CCGCTC CCGCTC(-3/-3)
1428 CCGCTC
BsrI 1761 ACTGG ACTGG(1/-1)
960 CCAGT
CelII 141 GCTAAGC GC^T[ACGT]AGC
585 GCTGAGC
EagI 5 CGGCCG C^GGCCG
17 CGGCCG
EclXI 5 CGGCCG C^GGCCG
17 CGGCCG
Eco52I 5 CGGCCG C^GGCCG
17 CGGCCG
EcoT14I 1547 CCAAGG C^C[AT][AT]GG
1553 CCAAGG
Hin1I 74 GACGCC G[GA]^CG[CT]C
956 GACGCC
MluNI 470 TGGCCA TGG^CCA
836 TGGCCA
MscI 470 TGGCCA TGG^CCA
836 TGGCCA
MseI 376 TTAA T^TAA
515 TTAA
PleI 1678 GACTC GAGTC(4/5)
1658 GAGTC
PstI 343 CTGCAG CTGCA^G
1559 CTGCAG
PvuII 256 CAGCTG CAG^CTG
1631 CAGCTG
RsaI 1601 GTAC GT^AC
1699 GTAC
SfcI 343 CTGCAG C^T[GA][CT]AG
1559 CTGCAG
StyI 1547 CCAAGG C^C[AT][AT]GG
1553 CCAAGG
Tru9I 376 TTAA T^TAA
515 TTAA
XmnI 502 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1696 GAAGTACTTC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BsaJI BsiEI BsiYI
BslI BsmAI BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I