Restriction map for
pF1KSDA0053
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsiWI BspDI BspLU11I BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI Eam1105I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1230 TCCGGA T^CCGGA
AlwI 990 GATCC GGATC(4/5)
AocI 414 CCTCAGG CC^T[ACGT]AGG
AvaI 1826 CCCGGG C^[CT]CG[GA]G
BanII 709 GAGCCC G[GA]GC[CT]^C
BbvCI 186 GCTGAGG CCTCAGC(-5/-2)
BcgI 954 CGAAGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1367 AGATCT A^GATCT
BlpI 784 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 784 GCTCAGC GC^T[ACGT]AGC
BseAI 1230 TCCGGA T^CCGGA
BsgI 527 GTGCAG GTGCAG(16/14)
BsiEI 549 CGGCCG CG[GA][CT]^CG
BsmBI 611 GAGACG CGTCTC(1/5)
BsmI 765 GAATGC GAATGC(1/-1)
BsoBI 1826 CCCGGG C^[CT]CG[GA]G
Bsp1407I 262 TGTACA T^GTACA
BspEI 1230 TCCGGA T^CCGGA
BspHI 971 TCATGA T^CATGA
BspMI 854 GCAGGT ACCTGC(4/8)
BsrBI 631 GAGCGG CCGCTC(-3/-3)
BsrGI 262 TGTACA T^GTACA
BssSI 821 CTCGTG CACGAG(-5/-1)
Bsu36I 414 CCTCAGG CC^T[ACGT]AGG
CelII 784 GCTCAGC GC^T[ACGT]AGC
DraI 1372 TTTAAA TTT^AAA
DraII 1507 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 713 CCGTGG C^C[GA][CT]GG
EagI 549 CGGCCG C^GGCCG
EclXI 549 CGGCCG C^GGCCG
Eco52I 549 CGGCCG C^GGCCG
Eco81I 414 CCTCAGG CC^T[ACGT]AGG
EcoNI 1710 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1507 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1381 GAATTC G^AATTC
FauI 105 CCCGC CCCGC(4/6)
HgaI 613 GACGC GACGC(5/10)
HincII 889 GTTAAC GT[CT]^[GA]AC
HindII 889 GTTAAC GT[CT]^[GA]AC
HpaI 889 GTTAAC GTT^AAC
MroI 1230 TCCGGA T^CCGGA
NspI 245 GCATGT [GA]CATG^[CT]
PflMI 453 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1507 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 971 TCATGA T^CATGA
SexAI 593 ACCTGGT A^CC[AT]GGT
SmaI 1826 CCCGGG CCC^GGG
SspBI 262 TGTACA T^GTACA
StuI 1853 AGGCCT AGG^CCT
Van91I 453 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1826 CCCGGG C^CCGGG
XmaI 1826 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 661 GTGCAC G^TGCAC
1529 GTGCAC
Aor51HI 455 AGCGCT AGC^GCT
1820 AGCGCT
ApaLI 661 GTGCAC G^TGCAC
1529 GTGCAC
Asp700I 316 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1317 GAAATGTTTC
BalI 374 TGGCCA TGG^CCA
450 TGGCCA
BanI 504 GGTGCC G^G[CT][GA]CC
1345 GGTGCC
BbsI 955 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BclI 968 TGATCA T^GATCA
1013 TGATCA
BcnI 1826 CCCGG CC^[GC]GG
1827 CCGGG
BfaI 1151 CTAG C^TAG
1795 CTAG
BpuAI 955 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BsaI 698 GAGACC GGTCTC(1/5)
1019 GAGACC
BseRI 983 CTCCTC GAGGAG(10/8)
397 GAGGAG
BstYI 989 AGATCC [GA]^GATC[CT]
1367 AGATCT
DraIII 434 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
664 CACACTGTG
DrdI 358 GACTCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1021 GACCATGAAGTC
Eco47III 455 AGCGCT AGC^GCT
1820 AGCGCT
FbaI 968 TGATCA T^GATCA
1013 TGATCA
HaeII 455 AGCGCT [GA]GCGC^[CT]
1820 AGCGCT
MaeI 1151 CTAG C^TAG
1795 CTAG
MaeII 1490 ACGT A^CGT
1730 ACGT
MflI 989 AGATCC [GA]^GATC[CT]
1367 AGATCT
MluNI 374 TGGCCA TGG^CCA
450 TGGCCA
MscI 374 TGGCCA TGG^CCA
450 TGGCCA
NciI 1826 CCCGG CC^[GC]GG
1827 CCGGG
PstI 852 CTGCAG CTGCA^G
1787 CTGCAG
PvuII 604 CAGCTG CAG^CTG
874 CAGCTG
SmlI 1439 CTCAAG C^T[CT][GA]AG
1711 CTTGAG
TaqI 946 TCGA T^CGA
953 TCGA
TthHB8I 946 TCGA T^CGA
953 TCGA
XhoII 989 AGATCC [GA]^GATC[CT]
1367 AGATCT
XmnI 316 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1317 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbvI BmyI BpmI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI