Restriction map for
pF1KSDA0043
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AlwNI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI MamI MfeI MluI MroI
MslI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 756 GACGTC GACGT^C
AcsI 392 AAATTT [GA]^AATT[CT]
AflIII 1217 ACGTGT A^C[GA][CT]GT
ApoI 392 AAATTT [GA]^AATT[CT]
Asp700I 1926 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1193 TGGCCA TGG^CCA
BamHI 32 GGATCC G^GATCC
BbsI 141 GAAGAC GAAGAC(2/6)
BbvCI 1071 CCTCAGC CCTCAGC(-5/-2)
BcgI 1957 GCAGCCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 141 GAAGAC GAAGAC(2/6)
BsaI 75 GGTCTC GGTCTC(1/5)
BsgI 1033 CTGCAC GTGCAG(16/14)
BsmBI 567 CGTCTC CGTCTC(1/5)
BsrBI 1372 GAGCGG CCGCTC(-3/-3)
BstAPI 117 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 32 GGATCC [GA]^GATC[CT]
DpnII 33 GATC ^GATC
DraIII 1531 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 380 AAGCTT A^AGCTT
MboI 33 GATC ^GATC
MflI 32 GGATCC [GA]^GATC[CT]
MluNI 1193 TGGCCA TGG^CCA
MscI 1193 TGGCCA TGG^CCA
NdeII 33 GATC ^GATC
NheI 1977 GCTAGC G^CTAGC
NspI 122 ACATGC [GA]CATG^[CT]
PflMI 180 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 872 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 576 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1335 GAGCTC GAGCT^C
SapI 1337 GCTCTTC GCTCTTC(1/4)
Sau3AI 33 GATC ^GATC
SgrAI 1265 CACCGGCG C[GA]^CCGG[CT]G
Van91I 180 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 32 GGATCC [GA]^GATC[CT]
XmnI 1926 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 576 GACGCC G[GA]^CG[CT]C
756 GACGTC
Alw44I 1417 GTGCAC G^TGCAC
1786 GTGCAC
AlwI 33 GATCC GGATC(4/5)
32 GGATC
AocI 954 CCTCAGG CC^T[ACGT]AGG
2084 CCTCAGG
ApaI 48 GGGCCC GGGCC^C
2091 GGGCCC
ApaLI 1417 GTGCAC G^TGCAC
1786 GTGCAC
BanI 891 GGTGCC G^G[CT][GA]CC
1263 GGCACC
BfaI 268 CTAG C^TAG
1978 CTAG
BsaHI 576 GACGCC G[GA]^CG[CT]C
756 GACGTC
BssSI 1183 CACGAG CACGAG(-5/-1)
1420 CACGAG
BstXI 818 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1379 CCACCAGGCTGG
Bsu36I 954 CCTCAGG CC^T[ACGT]AGG
2084 CCTCAGG
Eco81I 954 CCTCAGG CC^T[ACGT]AGG
2084 CCTCAGG
EcoNI 1437 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1811 CCTCGCTCAGG
Hin1I 576 GACGCC G[GA]^CG[CT]C
756 GACGTC
HphI 138 GGTGA GGTGA(8/7)
1296 GGTGA
KspI 26 CCGCGG CCGC^GG
1283 CCGCGG
MaeI 268 CTAG C^TAG
1978 CTAG
MseI 230 TTAA T^TAA
257 TTAA
NaeI 474 GCCGGC GCC^GGC
1636 GCCGGC
NcoI 1196 CCATGG C^CATGG
1292 CCATGG
NgoMIV 474 GCCGGC G^CCGGC
1636 GCCGGC
SacII 26 CCGCGG CCGC^GG
1283 CCGCGG
SmaI 45 CCCGGG CCC^GGG
1762 CCCGGG
TaqI 1153 TCGA T^CGA
1966 TCGA
Tru9I 230 TTAA T^TAA
257 TTAA
Tsp45I 622 GTCAC ^GT[GC]AC
2168 GTGAC
TthHB8I 1153 TCGA T^CGA
1966 TCGA
XcmI 674 CCATCGTCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
866 CCAAGAAGGACCTGG
XmaCI 45 CCCGGG C^CCGGG
1762 CCCGGG
XmaI 45 CCCGGG C^CCGGG
1762 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
PvuII RsaI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp509I TspRI