Restriction map for
pF1KSDA0037
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI AvrII BamHI BciVI
BfrI BglII BlnI BseAI BspDI
BspEI BspHI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DrdI Eco47III EcoRI EcoRV
EcoT22I FseI HindIII HpaI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AhdI 1177 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1240 GTGCAC G^TGCAC
ApaLI 1240 GTGCAC G^TGCAC
Asp700I 2484 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1177 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2626 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 580 GGCGCC GGCGC^C
BbrPI 2566 CACGTG CAC^GTG
BcgI 1817 GCATCCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3158 TGATCA T^GATCA
BfaI 2363 CTAG C^TAG
BsaBI 1332 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1208 ACCGGA [AT]^CCGG[AT]
BsmBI 456 CGTCTC CGTCTC(1/5)
BsmI 3145 GAATGC GAATGC(1/-1)
Bsp1407I 407 TGTACA T^GTACA
BspLU11I 1058 ACATGT A^CATGT
BsrBI 2863 GAGCGG CCGCTC(-3/-3)
BsrGI 407 TGTACA T^GTACA
BstAPI 594 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 1233 CGGCCG C^GGCCG
Eam1105I 1177 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1233 CGGCCG C^GGCCG
Eco52I 1233 CGGCCG C^GGCCG
FbaI 3158 TGATCA T^GATCA
HincII 2689 GTCAAC GT[CT]^[GA]AC
HindII 2689 GTCAAC GT[CT]^[GA]AC
KasI 580 GGCGCC G^GCGCC
MaeI 2363 CTAG C^TAG
MamI 1332 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2596 TTAA T^TAA
NarI 580 GGCGCC GG^CGCC
PflFI 2626 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2566 CACGTG CAC^GTG
PmlI 2566 CACGTG CAC^GTG
PshAI 1530 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2271 GCTCTTC GCTCTTC(1/4)
SfiI 2250 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 580 GGCGCC GGC^GCC
SspBI 407 TGTACA T^GTACA
TfiI 965 GAATC G^A[AT]TC
Tru9I 2596 TTAA T^TAA
Tsp509I 2398 AATT ^AATT
Tth111I 2626 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2484 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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Acc65I 1457 GGTACC G^GTACC
2609 GGTACC
AcyI 580 GGCGCC G[GA]^CG[CT]C
1437 GGCGTC
AlwI 651 GATCC GGATC(4/5)
972 GATCC
AocI 2208 CCTGAGG CC^T[ACGT]AGG
2837 CCTCAGG
Asp718I 1457 GGTACC G^GTACC
2609 GGTACC
BbvCI 1998 GCTGAGG CCTCAGC(-5/-2)
3183 GCTGAGG
BlpI 1665 GCTCAGC GC^T[ACGT]AGC
2826 GCTCAGC
Bpu1102I 1665 GCTCAGC GC^T[ACGT]AGC
2826 GCTCAGC
BsaAI 2566 CACGTG [CT]AC^GT[GA]
2805 CACGTA
BsaHI 580 GGCGCC G[GA]^CG[CT]C
1437 GGCGTC
BsgI 1962 CTGCAC GTGCAG(16/14)
3111 CTGCAC
BsiEI 1233 CGGCCG CG[GA][CT]^CG
1615 CGGTCG
BsiWI 1280 CGTACG C^GTACG
1631 CGTACG
BstEII 769 GGTGACC G^GT[ACGT]ACC
3096 GGTTACC
BstPI 769 GGTGACC G^GT[ACGT]ACC
3096 GGTTACC
BstYI 650 AGATCC [GA]^GATC[CT]
971 AGATCC
Bsu36I 2208 CCTGAGG CC^T[ACGT]AGG
2837 CCTCAGG
CelII 1665 GCTCAGC GC^T[ACGT]AGC
2826 GCTCAGC
EarI 622 CTCTTC CTCTTC(1/4)
2272 CTCTTC
Eco81I 2208 CCTGAGG CC^T[ACGT]AGG
2837 CCTCAGG
EcoO65I 769 GGTGACC G^GT[ACGT]ACC
3096 GGTTACC
Hin1I 580 GGCGCC G[GA]^CG[CT]C
1437 GGCGTC
KpnI 1457 GGTACC GGTAC^C
2609 GGTACC
Ksp632I 622 CTCTTC CTCTTC(1/4)
2272 CTCTTC
MflI 650 AGATCC [GA]^GATC[CT]
971 AGATCC
PaeR7I 1462 CTCGAG C^TCGAG
1932 CTCGAG
PleI 1627 GACTC GAGTC(4/5)
1465 GAGTC
SacI 916 GAGCTC GAGCT^C
2749 GAGCTC
SmaI 614 CCCGGG CCC^GGG
1772 CCCGGG
Tsp45I 770 GTGAC ^GT[GC]AC
2588 GTGAC
XhoI 1462 CTCGAG C^TCGAG
1932 CTCGAG
XhoII 650 AGATCC [GA]^GATC[CT]
971 AGATCC
XmaCI 614 CCCGGG C^CCGGG
1772 CCCGGG
XmaI 614 CCCGGG C^CCGGG
1772 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI ApaI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TseI TspRI TthHB8I
Van91I XcmI