Restriction map for
pF1KSDA0035
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DraIII DsaI Eam1105I
Eco47III EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KspI MaeII MamI MboI MfeI
MflI MluI MroI MslI MunI
NarI NcoI NdeI NdeII NheI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI Sau3AI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 1174 GGTACC G^GTACC
AcsI 1185 GAATTC [GA]^AATT[CT]
ApoI 1185 GAATTC [GA]^AATT[CT]
Asp718I 1174 GGTACC G^GTACC
AvaI 608 CTCGAG C^[CT]CG[GA]G
BciVI 42 GTATCC GTATCC(6/5)
BfaI 824 CTAG C^TAG
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmI 14 GCATTC GAATGC(1/-1)
BsoBI 608 CTCGAG C^[CT]CG[GA]G
BsrFI 10 GCCGGC [GA]^CCGG[CT]
CfoI 66 GCGC GCG^C
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
DrdI 1482 GACATCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoRI 1185 GAATTC G^AATTC
HhaI 66 GCGC GCG^C
HinP1I 66 GCGC G^CGC
KpnI 1174 GGTACC GGTAC^C
MaeI 824 CTAG C^TAG
NaeI 10 GCCGGC GCC^GGC
NgoMIV 10 GCCGGC G^CCGGC
NotI 4 GCGGCCGC GC^GGCCGC
PaeR7I 608 CTCGAG C^TCGAG
SapI 1112 GCTCTTC GCTCTTC(1/4)
SexAI 885 ACCAGGT A^CC[AT]GGT
StuI 242 AGGCCT AGG^CCT
TaqI 609 TCGA T^CGA
TthHB8I 609 TCGA T^CGA
XhoI 608 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 22 CGCG CG^CG
67 CGCG
AocI 1417 CCTCAGG CC^T[ACGT]AGG
1822 CCTCAGG
BanI 1174 GGTACC G^G[CT][GA]CC
1672 GGCACC
BcnI 812 CCCGG CC^[GC]GG
2076 CCGGG
BspMI 321 ACCTGC ACCTGC(4/8)
1221 ACCTGC
BssSI 1940 CACGAG CACGAG(-5/-1)
49 CTCGTG
BstAPI 1522 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1681 GCACTGACTGC
BstUI 22 CGCG CG^CG
67 CGCG
Bsu36I 1417 CCTCAGG CC^T[ACGT]AGG
1822 CCTCAGG
Eco81I 1417 CCTCAGG CC^T[ACGT]AGG
1822 CCTCAGG
FauI 909 CCCGC CCCGC(4/6)
2064 GCGGG
HindIII 1273 AAGCTT A^AGCTT
1840 AAGCTT
MvnI 22 CGCG CG^CG
67 CGCG
NciI 812 CCCGG CC^[GC]GG
2076 CCGGG
NlaIII 873 CATG CATG^
2044 CATG
PpuMI 185 AGGTCCC [GA]G^G[AT]CC[CT]
888 AGGTCCC
SacI 495 GAGCTC GAGCT^C
1110 GAGCTC
XcmI 956 CCAAGAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1247 CCAAGCAACCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BanII BbsI BbvI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NlaIV PleI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI