Restriction map for
pF1KSDA0033
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbsI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstYI Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeIII HapII
HincII HindII HindIII HpaI HpaII
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspI MunI NaeI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 93 GGCGCC G[GA]^CG[CT]C
AhdI 78 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 142 GGATC GGATC(4/5)
AseI 643 ATTAAT AT^TAAT
AsnI 643 ATTAAT AT^TAAT
AspEI 78 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 93 GGCGCC GGCGC^C
BbvCI 226 CCTCAGC CCTCAGC(-5/-2)
BglI 730 GCCATTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 93 GGCGCC G[GA]^CG[CT]C
BsiEI 25 CGATCG CG[GA][CT]^CG
BspMI 564 GCAGGT ACCTGC(4/8)
BsrI 517 CCAGT ACTGG(1/-1)
BstF5I 276 GGATG GGATG(2/0)
BstXI 266 CCAGAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 834 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 56 CCGTGG C^C[GA][CT]GG
Eam1105I 78 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 284 CCAAGG C^C[AT][AT]GG
FokI 276 GGATG GGATG(9/13)
HgaI 78 GACGC GACGC(5/10)
Hin1I 93 GGCGCC G[GA]^CG[CT]C
HinfI 14 GAGTC G^A[ACGT]TC
HphI 42 TCACC GGTGA(8/7)
KasI 93 GGCGCC G^GCGCC
MspA1I 71 CAGCGG C[AC]G^C[GT]G
NarI 93 GGCGCC GG^CGCC
PleI 14 GAGTC GAGTC(4/5)
PshBI 643 ATTAAT AT^TAAT
PstI 188 CTGCAG CTGCA^G
PvuI 25 CGATCG CGAT^CG
ScaI 336 AGTACT AGT^ACT
SfaNI 280 GATGC GCATC(5/9)
SfcI 188 CTGCAG C^T[GA][CT]AG
SfoI 93 GGCGCC GGC^GCC
SspI 795 AATATT AAT^ATT
StyI 284 CCAAGG C^C[AT][AT]GG
TspRI 749 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 407 GTATAC GT^[AC][GT]AC
523 GTATAC
AccII 80 CGCG CG^CG
90 CGCG
AcsI 219 AAATTT [GA]^AATT[CT]
865 AAATTT
AfaI 337 GTAC GT^AC
695 GTAC
ApoI 219 AAATTT [GA]^AATT[CT]
865 AAATTT
BanI 93 GGCGCC G^G[CT][GA]CC
475 GGTGCC
BsmFI 62 GGGAC GGGAC(10/14)
76 GGGAC
Bst1107I 407 GTATAC GTA^TAC
523 GTATAC
BstUI 80 CGCG CG^CG
90 CGCG
BstZ17I 407 GTATAC GTA^TAC
523 GTATAC
CfoI 38 GCGC GCG^C
94 GCGC
FauI 73 GCGGG CCCGC(4/6)
81 GCGGG
HaeII 37 GGCGCT [GA]GCGC^[CT]
93 GGCGCC
HhaI 38 GCGC GCG^C
94 GCGC
HinP1I 38 GCGC G^CGC
94 GCGC
MaeII 573 ACGT A^CGT
668 ACGT
MaeIII 156 GTCAC ^GT[ACGT]AC
558 GTCAC
MvnI 80 CGCG CG^CG
90 CGCG
RsaI 337 GTAC GT^AC
695 GTAC
Tsp45I 156 GTCAC ^GT[GC]AC
558 GTCAC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BbvI BfaI BsaI
BsaJI BsiYI BslI BsmAI BssKI
BstNI DdeI DpnII EcoRII Fnu4HI
ItaI MaeI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIV
Sau3AI ScrFI Tru9I TseI Tsp509I