Restriction map for
pF1KSDA0024
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BlnI BsaAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraII DrdI DsaI
Eam1105I Eco47III Eco81I EcoO109I EcoRV
EcoT14I FauI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccII 1045 CGCG CG^CG
AccIII 62 TCCGGA T^CCGGA
AcyI 3 GGCGTC G[GA]^CG[CT]C
Asp700I 906 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1264 GGCACC G^G[CT][GA]CC
BanII 598 GAGCTC G[GA]GC[CT]^C
BcnI 770 CCGGG CC^[GC]GG
BglI 529 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 135 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 135 GCTCAGC GC^T[ACGT]AGC
BsaBI 400 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaI 559 GGTCTC GGTCTC(1/5)
BsaWI 62 TCCGGA [AT]^CCGG[AT]
BseAI 62 TCCGGA T^CCGGA
BseRI 616 CTCCTC GAGGAG(10/8)
BsiEI 115 CGGCCG CG[GA][CT]^CG
BsmFI 1303 GGGAC GGGAC(10/14)
BsmI 1380 GCATTC GAATGC(1/-1)
BspEI 62 TCCGGA T^CCGGA
BspHI 878 TCATGA T^CATGA
BspMI 372 GCAGGT ACCTGC(4/8)
BsrDI 674 GCAATG GCAATG(2/0)
BstEII 1228 GGTCACC G^GT[ACGT]ACC
BstPI 1228 GGTCACC G^GT[ACGT]ACC
BstUI 1045 CGCG CG^CG
CelII 135 GCTCAGC GC^T[ACGT]AGC
CfoI 1044 GCGC GCG^C
DraIII 1032 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 115 CGGCCG [CT]^GGCC[GA]
EagI 115 CGGCCG C^GGCCG
EclXI 115 CGGCCG C^GGCCG
Eco52I 115 CGGCCG C^GGCCG
EcoO65I 1228 GGTCACC G^GT[ACGT]ACC
EcoRI 968 GAATTC G^AATTC
HgaI 4 GCGTC GACGC(5/10)
HhaI 1044 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 1044 GCGC G^CGC
HindIII 744 AAGCTT A^AGCTT
MamI 400 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 62 TCCGGA T^CCGGA
MslI 246 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 892 CAGCTG C[AC]G^C[GT]G
MvnI 1045 CGCG CG^CG
NciI 770 CCGGG CC^[GC]GG
NheI 808 GCTAGC G^CTAGC
NspI 1335 GCATGC [GA]CATG^[CT]
PstI 793 CTGCAG CTGCA^G
PvuII 892 CAGCTG CAG^CTG
RcaI 878 TCATGA T^CATGA
SacI 598 GAGCTC GAGCT^C
SapI 600 GCTCTTC GCTCTTC(1/4)
ScaI 1010 AGTACT AGT^ACT
SfiI 528 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1335 GCATGC GCATG^C
StuI 536 AGGCCT AGG^CCT
TfiI 1374 GAATC G^A[AT]TC
XcmI 1276 CCAGAGATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 906 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 118 CCGC CCGC(-3/-1)
680 GCGG
AspHI 325 GTGCTC G[AT]GC[AT]^C
598 GAGCTC
BbsI 196 GAAGAC GAAGAC(2/6)
1318 GAAGAC
BbvI 78 GCAGC GCAGC(8/12)
891 GCAGC
BfaI 397 CTAG C^TAG
809 CTAG
BglII 1119 AGATCT A^GATCT
1280 AGATCT
BmyI 325 GTGCTC G[AGT]GC[ACT]^C
598 GAGCTC
BpuAI 196 GAAGAC GAAGAC(2/6)
1318 GAAGAC
BsiHKAI 325 GTGCTC G[AT]GC[AT]^C
598 GAGCTC
BsmAI 1005 GAGAC GTCTC(1/5)
560 GTCTC
Bsp1286I 325 GTGCTC G[AGT]GC[ACT]^C
598 GAGCTC
BsrFI 113 ACCGGC [GA]^CCGG[CT]
710 GCCGGT
BsrI 737 ACTGG ACTGG(1/-1)
944 CCAGT
BstXI 292 CCAGCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCATTAAGTTGG
Cfr10I 113 ACCGGC [GA]^CCGG[CT]
710 GCCGGT
EcoT22I 55 ATGCAT ATGCA^T
610 ATGCAT
MaeI 397 CTAG C^TAG
809 CTAG
MseI 377 TTAA T^TAA
955 TTAA
NsiI 55 ATGCAT ATGCA^T
610 ATGCAT
PleI 184 GACTC GAGTC(4/5)
317 GACTC
SfcI 793 CTGCAG C^T[GA][CT]AG
1269 CTACAG
Tru9I 377 TTAA T^TAA
955 TTAA
TseI 78 GCAGC G^C[AT]GC
891 GCAGC
Tsp45I 1229 GTCAC ^GT[GC]AC
1396 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BclI BpmI BsaJI BsiYI
BslI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoNI EcoRII FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI Tsp509I
TspRI TthHB8I XhoII