##################################
# NO CUT ENZYMES (  190 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvaI        
NaeI        NarI        NdeI        NdeII       NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        TaqI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AluI          465  AGCT                 AG^CT
BbsI          643  GAAGAC               GAAGAC(2/6)
BciVI         228  GGATAC               GTATCC(6/5)
BcnI            6  CCGGG                CC^[GC]GG
BpuAI         643  GAAGAC               GAAGAC(2/6)
BsaJI         174  CCATGG               C^C[ACGT][ACGT]GG
BsgI          391  CTGCAC               GTGCAG(16/14)
Bsp1407I      575  TGTACA               T^GTACA
BsrBI         179  GAGCGG               CCGCTC(-3/-3)
BsrDI         243  GCAATG               GCAATG(2/0)
BsrGI         575  TGTACA               T^GTACA
BsrI          225  ACTGG                ACTGG(1/-1)
BssKI           6  CCGGG                ^CC[ACGT]GG
BstF5I        150  GGATG                GGATG(2/0)
DsaI          174  CCATGG               C^C[GA][CT]GG
EcoRV         262  GATATC               GAT^ATC
EcoT14I       174  CCATGG               C^C[AT][AT]GG
FauI           19  CCCGC                CCCGC(4/6)
FokI          150  GGATG                GGATG(9/13)
HapII           6  CCGG                 C^CGG
HincII        373  GTTGAC               GT[CT]^[GA]AC
HindII        373  GTTGAC               GT[CT]^[GA]AC
HpaII           6  CCGG                 C^CGG
MaeIII        107  GTTAC                ^GT[ACGT]AC
MseI          508  TTAA                 T^TAA
MspA1I         50  CCGCTG               C[AC]G^C[GT]G
MspI            6  CCGG                 C^CGG
NciI            6  CCGGG                CC^[GC]GG
NcoI          174  CCATGG               C^CATGG
NheI          529  GCTAGC               G^CTAGC
NlaIV         569  GGTTCC               GG[ACGT]^[ACGT]CC
NspI          275  GCATGT               [GA]CATG^[CT]
ScrFI           6  CCGGG                CC^[ACGT]GG
SfaNI         295  GATGC                GCATC(5/9)
SspBI         575  TGTACA               T^GTACA
SspI          485  AATATT               AAT^ATT
StyI          174  CCATGG               C^C[AT][AT]GG
Tru9I         508  TTAA                 T^TAA
TspRI         163  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          167  GTAC                 GT^AC
              576  GTAC                
BfaI          271  CTAG                 C^TAG
              530  CTAG                
BsiYI          34  CCGTTGCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              174  CCATGGAGCGG         
BslI           34  CCGTTGCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              174  CCATGGAGCGG         
Cac8I          18  GCCCGC               GC[ACGT]^[ACGT]GC
              529  GCTAGC              
CfoI           13  GCGC                 GCG^C
               22  GCGC                
HhaI           13  GCGC                 GCG^C
               22  GCGC                
HinP1I         13  GCGC                 G^CGC
               22  GCGC                
HinfI          87  GATTC                G^A[ACGT]TC
              592  GATTC               
MaeI          271  CTAG                 C^TAG
              530  CTAG                
RsaI          167  GTAC                 GT^AC
              576  GTAC                
TfiI           87  GATTC                G^A[AT]TC
              592  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AccII       AciI        BbvI        BstUI       Cfr13I      
Fnu4HI      HaeIII      HphI        ItaI        MboII       
MnlI        MvnI        MwoI        NlaIII      Sau96I      
TseI        Tsp509I