Restriction map for
pF1KSDA0016
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssKI BssSI Bst1107I BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FokI FseI FspI HgaI
Hin1I HincII HindII HinfI HpaI
KasI KpnI KspI MaeII MamI
MboI MflI MluI MluNI MnlI
MroI MscI MslI MspA1I MvaI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI TseI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 268 GTAGAC GT^[AC][GT]AC
AfaI 57 GTAC GT^AC
AlwNI 313 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 41 GGGCCC GGGCC^C
AspHI 404 GTGCTC G[AT]GC[AT]^C
BanII 41 GGGCCC G[GA]GC[CT]^C
BcgI 62 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 241 CTAG C^TAG
BsaJI 143 CCAAGG C^C[ACGT][ACGT]GG
BsiEI 72 CGACCG CG[GA][CT]^CG
BsiHKAI 404 GTGCTC G[AT]GC[AT]^C
BspMI 321 GCAGGT ACCTGC(4/8)
BsrFI 28 GCCGGT [GA]^CCGG[CT]
BsrI 349 CCAGT ACTGG(1/-1)
BstAPI 312 GCAGTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 138 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 17 GCGC GCG^C
Cfr10I 28 GCCGGT [GA]^CCGG[CT]
EcoT14I 143 CCAAGG C^C[AT][AT]GG
FauI 38 GCGGG CCCGC(4/6)
Fnu4HI 25 GCCGC GC^[ACGT]GC
HaeII 16 AGCGCC [GA]GCGC^[CT]
HaeIII 42 GGCC GG^CC
HapII 29 CCGG C^CGG
HhaI 17 GCGC GCG^C
HinP1I 17 GCGC G^CGC
HindIII 136 AAGCTT A^AGCTT
HpaII 29 CCGG C^CGG
ItaI 25 GCCGC GC^[ACGT]GC
MaeI 241 CTAG C^TAG
MfeI 287 CAATTG C^AATTG
MseI 179 TTAA T^TAA
MspI 29 CCGG C^CGG
MunI 287 CAATTG C^AATTG
PstI 319 CTGCAG CTGCA^G
RsaI 57 GTAC GT^AC
SfcI 319 CTGCAG C^T[GA][CT]AG
SgrAI 27 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 245 CTCAAG C^T[CT][GA]AG
StyI 143 CCAAGG C^C[AT][AT]GG
Tru9I 179 TTAA T^TAA
Tsp45I 89 GTGAC ^GT[GC]AC
TspRI 350 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 16 enzymes) #
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Asp700I 190 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
201 GAAGTTCTTC
BmyI 41 GGGCCC G[AGT]GC[ACT]^C
404 GTGCTC
Bsp1286I 41 GGGCCC G[AGT]GC[ACT]^C
404 GTGCTC
Cfr13I 41 GGGCC G^G[ACGT]CC
42 GGCCC
DdeI 371 CTAAG C^T[ACGT]AG
407 CTCAG
DraII 40 GGGGCCC [GA]G^G[ACGT]CC[CT]
41 GGGCCCT
EarI 338 CTCTTC CTCTTC(1/4)
232 GAAGAG
EcoO109I 40 GGGGCCC [GA]G^G[ACGT]CC[CT]
41 GGGCCCT
HphI 229 GGTGA GGTGA(8/7)
250 GGTGA
Ksp632I 338 CTCTTC CTCTTC(1/4)
232 GAAGAG
MwoI 19 GCCATCGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
312 GCAGTTACTGC
NlaIV 40 GGGGCC GG[ACGT]^[ACGT]CC
41 GGGCCC
Sau96I 41 GGGCC G^G[ACGT]CC
42 GGCCC
TaqI 71 TCGA T^CGA
114 TCGA
TthHB8I 71 TCGA T^CGA
114 TCGA
XmnI 190 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
201 GAAGTTCTTC
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AciI AluI MaeIII MboII SfaNI
Tsp509I