Restriction map for
pF1KSDA0014
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AgeI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
AspI AvaI AvrII BbeI BcgI
BciVI BfrI BglII BlnI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 1187 GGTACC G^GTACC
AhdI 1116 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1279 GTGCAC G^TGCAC
ApaLI 1279 GTGCAC G^TGCAC
Asp718I 1187 GGTACC G^GTACC
AspEI 1116 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 620 TGCGCA TGC^GCA
BamHI 387 GGATCC G^GATCC
BbsI 126 GAAGAC GAAGAC(2/6)
BbvCI 604 GCTGAGG CCTCAGC(-5/-2)
BclI 716 TGATCA T^GATCA
BpuAI 126 GAAGAC GAAGAC(2/6)
BsaWI 1184 TCCGGT [AT]^CCGG[AT]
BsmAI 1181 GTCTC GTCTC(1/5)
BsrBI 232 GAGCGG CCGCTC(-3/-3)
BsrDI 441 CATTGC GCAATG(2/0)
BstXI 485 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 387 GGATCC [GA]^GATC[CT]
DraIII 766 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1116 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 88 CTCTTC CTCTTC(1/4)
EcoT22I 438 ATGCAT ATGCA^T
FbaI 716 TGATCA T^GATCA
FspI 620 TGCGCA TGC^GCA
KpnI 1187 GGTACC GGTAC^C
Ksp632I 88 CTCTTC CTCTTC(1/4)
MaeIII 1034 GTAAC ^GT[ACGT]AC
MflI 387 GGATCC [GA]^GATC[CT]
NcoI 1217 CCATGG C^CATGG
NsiI 438 ATGCAT ATGCA^T
SacI 862 GAGCTC GAGCT^C
SbfI 225 CCTGCAGG CCTGCA^GG
SfiI 697 GGCCTGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 225 CCTGCAGG CCTGCA^GG
StuI 1346 AGGCCT AGG^CCT
TfiI 781 GATTC G^A[AT]TC
XhoII 387 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AflIII 155 ACACGT A^C[GA][CT]GT
726 ACACGT
AlwI 388 GATCC GGATC(4/5)
387 GGATC
AocI 967 CCTGAGG CC^T[ACGT]AGG
1409 CCTCAGG
BalI 749 TGGCCA TGG^CCA
1148 TGGCCA
BbrPI 156 CACGTG CAC^GTG
727 CACGTG
BfaI 239 CTAG C^TAG
1405 CTAG
BsaAI 156 CACGTG [CT]AC^GT[GA]
727 CACGTG
BseRI 876 CTCCTC GAGGAG(10/8)
1360 GAGGAG
BsrI 1455 ACTGG ACTGG(1/-1)
1178 CCAGT
Bsu36I 967 CCTGAGG CC^T[ACGT]AGG
1409 CCTCAGG
DpnII 388 GATC ^GATC
717 GATC
Eco81I 967 CCTGAGG CC^T[ACGT]AGG
1409 CCTCAGG
EcoT14I 1191 CCTTGG C^C[AT][AT]GG
1217 CCATGG
HphI 516 GGTGA GGTGA(8/7)
848 TCACC
MaeI 239 CTAG C^TAG
1405 CTAG
MaeII 157 ACGT A^CGT
728 ACGT
MboI 388 GATC ^GATC
717 GATC
MboII 126 GAAGA GAAGA(8/7)
89 TCTTC
MluNI 749 TGGCCA TGG^CCA
1148 TGGCCA
MscI 749 TGGCCA TGG^CCA
1148 TGGCCA
NdeII 388 GATC ^GATC
717 GATC
PflMI 741 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAGCTGTTGG
PleI 1167 GACTC GAGTC(4/5)
1246 GACTC
PmaCI 156 CACGTG CAC^GTG
727 CACGTG
PmlI 156 CACGTG CAC^GTG
727 CACGTG
Sau3AI 388 GATC ^GATC
717 GATC
SphI 251 GCATGC GCATG^C
333 GCATGC
StyI 1191 CCTTGG C^C[AT][AT]GG
1217 CCATGG
Van91I 741 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAGCTGTTGG
XcmI 447 CCAGCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
765 CCACTATGTGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV NspI
PpuMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI SmlI TseI
TspRI