Restriction map for
pF1KB8006
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AclI AgeI AocI
AscI AseI AsnI Asp718I AspI
AvrII BbeI BbrPI BbsI BciVI
BclI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsiEI BsiWI BsmBI
BsmI BspDI BsrDI BsrFI BssHII
Bst1107I BstZ17I Bsu36I Cfr10I ClaI
DraI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 1822 GTCTAC GT^[AC][GT]AC
AccIII 314 TCCGGA T^CCGGA
AcyI 2274 GGCGTC G[GA]^CG[CT]C
AhdI 814 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2304 GTGCAC G^TGCAC
Aor51HI 84 AGCGCT AGC^GCT
ApaLI 2304 GTGCAC G^TGCAC
AspEI 814 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2084 TGCGCA TGC^GCA
BalI 276 TGGCCA TGG^CCA
BamHI 232 GGATCC G^GATCC
BanI 1627 GGTGCC G^G[CT][GA]CC
BglI 1246 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1384 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1384 GCTGAGC GC^T[ACGT]AGC
BsaHI 2274 GGCGTC G[GA]^CG[CT]C
BsaWI 314 TCCGGA [AT]^CCGG[AT]
BseAI 314 TCCGGA T^CCGGA
Bsp1407I 407 TGTACA T^GTACA
BspEI 314 TCCGGA T^CCGGA
BspHI 923 TCATGA T^CATGA
BspLU11I 2058 ACATGT A^CATGT
BsrGI 407 TGTACA T^GTACA
BstAPI 1977 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1319 TTCGAA TT^CGAA
BstXI 933 CCATAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1384 GCTGAGC GC^T[ACGT]AGC
CpoI 311 CGGTCCG CG^G[AT]CCG
DraIII 1978 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1813 GACTCGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 814 GACCATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 84 AGCGCT AGC^GCT
EcoT14I 1893 CCATGG C^C[AT][AT]GG
FspI 2084 TGCGCA TGC^GCA
HaeII 84 AGCGCT [GA]GCGC^[CT]
HgaI 2275 GCGTC GACGC(5/10)
Hin1I 2274 GGCGTC G[GA]^CG[CT]C
MluNI 276 TGGCCA TGG^CCA
MroI 314 TCCGGA T^CCGGA
MscI 276 TGGCCA TGG^CCA
MslI 2053 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1893 CCATGG C^CATGG
NheI 2223 GCTAGC G^CTAGC
NspV 1319 TTCGAA TT^CGAA
PflMI 1893 CCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 191 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 2044 CAGCTG CAG^CTG
RcaI 923 TCATGA T^CATGA
RsrII 311 CGGTCCG CG^G[AT]CCG
SacI 68 GAGCTC GAGCT^C
SexAI 982 ACCTGGT A^CC[AT]GGT
SfuI 1319 TTCGAA TT^CGAA
SmaI 1203 CCCGGG CCC^GGG
SphI 477 GCATGC GCATG^C
SspBI 407 TGTACA T^GTACA
StyI 1893 CCATGG C^C[AT][AT]GG
TfiI 1297 GAATC G^A[AT]TC
Van91I 1893 CCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 816 CCATCCGTCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1203 CCCGGG C^CCGGG
XmaI 1203 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflII 334 CTTAAG C^TTAAG
343 CTTAAG
AflIII 1907 ACACGT A^C[GA][CT]GT
2058 ACATGT
AlwNI 1244 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2044 CAGCTGCTG
Asp700I 1365 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1924 GAAGTGGTTC
BfrI 334 CTTAAG C^TTAAG
343 CTTAAG
BsrBI 25 CCGCTC CCGCTC(-3/-3)
1387 GAGCGG
BssSI 1549 CACGAG CACGAG(-5/-1)
1873 CACGAG
BstEII 978 GGTCACC G^GT[ACGT]ACC
1635 GGTGACC
BstPI 978 GGTCACC G^GT[ACGT]ACC
1635 GGTGACC
DsaI 1893 CCATGG C^C[GA][CT]GG
2309 CCGTGG
EcoNI 983 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1257 CCTGGGGCAGG
EcoO65I 978 GGTCACC G^GT[ACGT]ACC
1635 GGTGACC
MspA1I 904 CCGCTG C[AC]G^C[GT]G
2044 CAGCTG
PaeR7I 1309 CTCGAG C^TCGAG
1783 CTCGAG
StuI 698 AGGCCT AGG^CCT
759 AGGCCT
XhoI 1309 CTCGAG C^TCGAG
1783 CTCGAG
XmnI 1365 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1924 GAAGTGGTTC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BanII BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII