Restriction map for
pF1KB8005
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsiWI BsmBI BspDI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MluI
MluNI MscI MspA1I MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AlwNI 149 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1162 CCTCAGG CC^T[ACGT]AGG
ApaI 28 GGGCCC GGGCC^C
AseI 1263 ATTAAT AT^TAAT
AsnI 1263 ATTAAT AT^TAAT
BamHI 2029 GGATCC G^GATCC
BfaI 587 CTAG C^TAG
BglII 1248 AGATCT A^GATCT
BsaBI 1576 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2115 CTGCAC GTGCAG(16/14)
BsiEI 568 CGACCG CG[GA][CT]^CG
BsmFI 1093 GTCCC GGGAC(10/14)
BsmI 1081 GAATGC GAATGC(1/-1)
Bsp1407I 1936 TGTACA T^GTACA
BspHI 1229 TCATGA T^CATGA
BsrFI 570 ACCGGC [GA]^CCGG[CT]
BsrGI 1936 TGTACA T^GTACA
Bsu36I 1162 CCTCAGG CC^T[ACGT]AGG
Cfr10I 570 ACCGGC [GA]^CCGG[CT]
DraI 1011 TTTAAA TTT^AAA
Eco81I 1162 CCTCAGG CC^T[ACGT]AGG
EcoNI 1162 CCTCAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1661 ATGCAT ATGCA^T
HaeII 557 AGCGCC [GA]GCGC^[CT]
HincII 946 GTTGAC GT[CT]^[GA]AC
HindII 946 GTTGAC GT[CT]^[GA]AC
MaeI 587 CTAG C^TAG
MamI 1576 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1230 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1664 CATATG CA^TATG
NsiI 1661 ATGCAT ATGCA^T
PaeR7I 2036 CTCGAG C^TCGAG
PshAI 448 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1263 ATTAAT AT^TAAT
RcaI 1229 TCATGA T^CATGA
SacI 1867 GAGCTC GAGCT^C
ScaI 51 AGTACT AGT^ACT
SspBI 1936 TGTACA T^GTACA
SspI 581 AATATT AAT^ATT
StuI 1290 AGGCCT AGG^CCT
XcmI 1243 CCAAAAGATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2036 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1428 TCCGGA T^CCGGA
1578 TCCGGA
AcsI 1130 AAATTT [GA]^AATT[CT]
1273 AAATTT
AflIII 655 ACATGT A^C[GA][CT]GT
2224 ACATGT
ApoI 1130 AAATTT [GA]^AATT[CT]
1273 AAATTT
Asp700I 1672 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1819 GAAGTAATTC
AspHI 828 GAGCAC G[AT]GC[AT]^C
1867 GAGCTC
AvaI 2036 CTCGAG C^[CT]CG[GA]G
2082 CCCGAG
AvaII 889 GGTCC G^G[AT]CC
1635 GGACC
BanII 28 GGGCCC G[GA]GC[CT]^C
1867 GAGCTC
BciVI 1604 GGATAC GTATCC(6/5)
1709 GTATCC
BcnI 10 CCCGG CC^[GC]GG
1922 CCCGG
BpmI 749 CTCCAG CTGGAG(16/14)
1761 CTGGAG
BsaWI 1428 TCCGGA [AT]^CCGG[AT]
1578 TCCGGA
BseAI 1428 TCCGGA T^CCGGA
1578 TCCGGA
BseRI 746 CTCCTC GAGGAG(10/8)
690 GAGGAG
BsiHKAI 828 GAGCAC G[AT]GC[AT]^C
1867 GAGCTC
BsoBI 2036 CTCGAG C^[CT]CG[GA]G
2082 CCCGAG
BspEI 1428 TCCGGA T^CCGGA
1578 TCCGGA
BspLU11I 655 ACATGT A^CATGT
2224 ACATGT
BspMI 2126 ACCTGC ACCTGC(4/8)
1168 GCAGGT
BstXI 478 CCAGACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1449 CCACTTTGTTGG
CfoI 42 GCGC GCG^C
558 GCGC
EcoT14I 1096 CCAAGG C^C[AT][AT]GG
1553 CCTTGG
HhaI 42 GCGC GCG^C
558 GCGC
HinP1I 42 GCGC G^CGC
558 GCGC
HphI 72 GGTGA GGTGA(8/7)
1669 GGTGA
MroI 1428 TCCGGA T^CCGGA
1578 TCCGGA
MwoI 30 GCCCGTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
549 GCAATGGGAGC
NciI 10 CCCGG CC^[GC]GG
1922 CCCGG
NlaIV 28 GGGCCC GG[ACGT]^[ACGT]CC
2029 GGATCC
NspI 655 ACATGT [GA]CATG^[CT]
2224 ACATGT
PleI 2087 GACTC GAGTC(4/5)
695 GAGTC
SapI 880 GAAGAGC GCTCTTC(1/4)
1993 GAAGAGC
SexAI 68 ACCTGGT A^CC[AT]GGT
1112 ACCTGGT
SfaNI 2110 GATGC GCATC(5/9)
574 GCATC
SmlI 1609 CTTGAG C^T[CT][GA]AG
2036 CTCGAG
StyI 1096 CCAAGG C^C[AT][AT]GG
1553 CCTTGG
XmnI 1672 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1819 GAAGTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI BbsI
BbvI BmyI BpuAI BsaI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MfeI MflI MnlI
MseI MspI MunI MvaI NdeII
NlaIII PflMI PstI RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII