Restriction map for
pF1KB8001
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsgI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI HphI KpnI MamI MfeI
MflI MluI MroI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 445 GTAGAC GT^[AC][GT]AC
AccII 487 CGCG CG^CG
AfaI 156 GTAC GT^AC
ApaI 709 GGGCCC GGGCC^C
AspHI 751 GTGCTC G[AT]GC[AT]^C
AvaI 638 CCCGGG C^[CT]CG[GA]G
BalI 32 TGGCCA TGG^CCA
BanI 527 GGCGCC G^G[CT][GA]CC
BbeI 527 GGCGCC GGCGC^C
BbrPI 645 CACGTG CAC^GTG
BpmI 58 CTGGAG CTGGAG(16/14)
BsaAI 645 CACGTG [CT]AC^GT[GA]
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BseRI 544 GAGGAG GAGGAG(10/8)
BsiEI 727 CGGCCG CG[GA][CT]^CG
BsiHKAI 751 GTGCTC G[AT]GC[AT]^C
BsmAI 464 GAGAC GTCTC(1/5)
BsmFI 482 GGGAC GGGAC(10/14)
BsmI 198 GAATGC GAATGC(1/-1)
BsoBI 638 CCCGGG C^[CT]CG[GA]G
BsrDI 84 CATTGC GCAATG(2/0)
BstEII 252 GGTTACC G^GT[ACGT]ACC
BstPI 252 GGTTACC G^GT[ACGT]ACC
BstUI 487 CGCG CG^CG
CpoI 350 CGGACCG CG^G[AT]CCG
EagI 727 CGGCCG C^GGCCG
EclXI 727 CGGCCG C^GGCCG
Eco52I 727 CGGCCG C^GGCCG
EcoO65I 252 GGTTACC G^GT[ACGT]ACC
EcoT14I 608 CCATGG C^C[AT][AT]GG
HgaI 631 GACGC GACGC(5/10)
HincII 651 GTCAAC GT[CT]^[GA]AC
HindII 651 GTCAAC GT[CT]^[GA]AC
HinfI 659 GACTC G^A[ACGT]TC
KasI 527 GGCGCC G^GCGCC
KspI 486 CCGCGG CCGC^GG
MaeII 646 ACGT A^CGT
MaeIII 253 GTTAC ^GT[ACGT]AC
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
MvnI 487 CGCG CG^CG
NarI 527 GGCGCC GG^CGCC
NcoI 608 CCATGG C^CATGG
PleI 659 GACTC GAGTC(4/5)
PmaCI 645 CACGTG CAC^GTG
PmlI 645 CACGTG CAC^GTG
PstI 19 CTGCAG CTGCA^G
PvuII 704 CAGCTG CAG^CTG
RsaI 156 GTAC GT^AC
RsrII 350 CGGACCG CG^G[AT]CCG
SacII 486 CCGCGG CCGC^GG
SexAI 127 ACCAGGT A^CC[AT]GGT
SfcI 19 CTGCAG C^T[GA][CT]AG
SfoI 527 GGCGCC GGC^GCC
SmaI 638 CCCGGG CCC^GGG
StuI 770 AGGCCT AGG^CCT
StyI 608 CCATGG C^C[AT][AT]GG
XmaCI 638 CCCGGG C^CCGGG
XmaI 638 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 527 GGCGCC G[GA]^CG[CT]C
631 GACGCC
BanII 181 GGGCTC G[GA]GC[CT]^C
709 GGGCCC
BciVI 217 GTATCC GTATCC(6/5)
732 GTATCC
BfaI 370 CTAG C^TAG
617 CTAG
BsaHI 527 GGCGCC G[GA]^CG[CT]C
631 GACGCC
BsrI 194 ACTGG ACTGG(1/-1)
685 ACTGG
Cac8I 179 GCGGGC GC[ACGT]^[ACGT]GC
549 GCTGGC
DdeI 274 CTCAG C^T[ACGT]AG
627 CTCAG
DraII 137 AGGGCCT [GA]G^G[ACGT]CC[CT]
709 GGGCCCC
EcoO109I 137 AGGGCCT [GA]G^G[ACGT]CC[CT]
709 GGGCCCC
HaeII 527 GGCGCC [GA]GCGC^[CT]
688 GGCGCT
Hin1I 527 GGCGCC G[GA]^CG[CT]C
631 GACGCC
MaeI 370 CTAG C^TAG
617 CTAG
MwoI 9 GCCCGTTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
530 GCCACCATCGC
SfaNI 292 GATGC GCATC(5/9)
44 GCATC
TaqI 167 TCGA T^CGA
423 TCGA
TthHB8I 167 TCGA T^CGA
423 TCGA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstXI CfoI Cfr13I DpnII DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI