Restriction map for
pF1KB6004
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI Asp718I AspI AviII BanII
BbvCI BclI BfrI BglI BseAI
BsiEI BsiWI BsmBI BspEI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
CpoI DraII DraIII DsaI EagI
EclXI Eco52I EcoO109I FauI FbaI
FseI FspI HpaI KpnI KspI
MluI MroI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SbfI SexAI SfiI SfuI
SgrAI SmaI Sse8387I SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1361 GTCGAC GT^[AC][GT]AC
Aor51HI 1870 AGCGCT AGC^GCT
AvaI 23 CTCGAG C^[CT]CG[GA]G
AvrII 1477 CCTAGG C^CTAGG
BamHI 1049 GGATCC G^GATCC
BbeI 548 GGCGCC GGCGC^C
BbrPI 143 CACGTG CAC^GTG
BcgI 3585 CGAGGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1380 GTATCC GTATCC(6/5)
BcnI 823 CCCGG CC^[GC]GG
BlnI 1477 CCTAGG C^CTAGG
BlpI 4531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4531 GCTGAGC GC^T[ACGT]AGC
BsaWI 3048 ACCGGA [AT]^CCGG[AT]
BsgI 4832 CTGCAC GTGCAG(16/14)
BsoBI 23 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3030 TGTACA T^GTACA
BsrBI 1045 GAGCGG CCGCTC(-3/-3)
BsrFI 305 GCCGGT [GA]^CCGG[CT]
BsrGI 3030 TGTACA T^GTACA
BstAPI 2170 GCAATTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3211 GGTGACC G^GT[ACGT]ACC
BstPI 3211 GGTGACC G^GT[ACGT]ACC
CelII 4531 GCTGAGC GC^T[ACGT]AGC
Cfr10I 305 GCCGGT [GA]^CCGG[CT]
Eco47III 1870 AGCGCT AGC^GCT
EcoO65I 3211 GGTGACC G^GT[ACGT]ACC
EcoRV 2800 GATATC GAT^ATC
EcoT22I 1534 ATGCAT ATGCA^T
HgaI 5734 GCGTC GACGC(5/10)
KasI 548 GGCGCC G^GCGCC
MfeI 3320 CAATTG C^AATTG
MspA1I 4829 CAGCTG C[AC]G^C[GT]G
MunI 3320 CAATTG C^AATTG
NarI 548 GGCGCC GG^CGCC
NciI 823 CCCGG CC^[GC]GG
NdeI 3217 CATATG CA^TATG
NsiI 1534 ATGCAT ATGCA^T
PaeR7I 23 CTCGAG C^TCGAG
PmaCI 143 CACGTG CAC^GTG
PmlI 143 CACGTG CAC^GTG
PstI 4058 CTGCAG CTGCA^G
PvuII 4829 CAGCTG CAG^CTG
SalI 1361 GTCGAC G^TCGAC
ScaI 3391 AGTACT AGT^ACT
SfoI 548 GGCGCC GGC^GCC
SnaBI 677 TACGTA TAC^GTA
SphI 636 GCATGC GCATG^C
SspBI 3030 TGTACA T^GTACA
XhoI 23 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AcyI 548 GGCGCC G[GA]^CG[CT]C
5733 GGCGTC
AlwNI 3344 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
5000 CAGTGGCTG
Asp700I 1454 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3526 GAAGAAATTC
AspHI 53 GAGCAC G[AT]GC[AT]^C
140 GAGCAC
BalI 168 TGGCCA TGG^CCA
5879 TGGCCA
BanI 548 GGCGCC G^G[CT][GA]CC
5175 GGTGCC
BglII 5016 AGATCT A^GATCT
5511 AGATCT
BpmI 80 CTCCAG CTGGAG(16/14)
1984 CTCCAG
BsaAI 143 CACGTG [CT]AC^GT[GA]
677 TACGTA
BsaHI 548 GGCGCC G[GA]^CG[CT]C
5733 GGCGTC
BsiHKAI 53 GAGCAC G[AT]GC[AT]^C
140 GAGCAC
BsmI 533 GAATGC GAATGC(1/-1)
3401 GCATTC
BspDI 625 ATCGAT AT^CGAT
3023 ATCGAT
BspHI 267 TCATGA T^CATGA
4496 TCATGA
BspMI 2881 ACCTGC ACCTGC(4/8)
3226 ACCTGC
BssSI 50 CACGAG CACGAG(-5/-1)
4766 CTCGTG
CfoI 549 GCGC GCG^C
1871 GCGC
ClaI 625 ATCGAT AT^CGAT
3023 ATCGAT
DrdI 1364 GACAGTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2286 GACTTTAGAGTC
EaeI 168 TGGCCA [CT]^GGCC[GA]
5879 TGGCCA
EcoNI 2737 CCTAATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5022 CCTCAAACAGG
HaeII 548 GGCGCC [GA]GCGC^[CT]
1870 AGCGCT
HhaI 549 GCGC GCG^C
1871 GCGC
Hin1I 548 GGCGCC G[GA]^CG[CT]C
5733 GGCGTC
HinP1I 549 GCGC G^CGC
1871 GCGC
MluNI 168 TGGCCA TGG^CCA
5879 TGGCCA
MscI 168 TGGCCA TGG^CCA
5879 TGGCCA
MslI 1192 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1274 CACATTTGTG
PflMI 2050 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2782 CCATTTGGTGG
RcaI 267 TCATGA T^CATGA
4496 TCATGA
SpeI 4126 ACTAGT A^CTAGT
4135 ACTAGT
Van91I 2050 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2782 CCATTTGGTGG
XcmI 248 CCAAAGAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4334 CCAGGTCAGGCTTGG
XmnI 1454 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3526 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AhdI
AluI AlwI AocI ApoI AseI
AsnI AspEI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaBI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
Eam1105I EarI Eco81I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII