Restriction map for
pF1KB4919
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AviII BbrPI BcgI BfrI
BglI BsaBI BsiEI BsiWI BspHI
BssHII BssSI Bst1107I BstBI BstZ17I
EagI EclXI Eco47III Eco52I EcoRI
FseI FspI HpaI KpnI MamI
MluI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 5279 GTAGAC GT^[AC][GT]AC
AccIII 5168 TCCGGA T^CCGGA
AcyI 4012 GGCGCC G[GA]^CG[CT]C
AflIII 5256 ACATGT A^C[GA][CT]GT
AhdI 511 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5252 GTGCAC G^TGCAC
ApaLI 5252 GTGCAC G^TGCAC
AspEI 511 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4783 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2456 TGGCCA TGG^CCA
BbeI 4012 GGCGCC GGCGC^C
BciVI 1788 GTATCC GTATCC(6/5)
BglII 4401 AGATCT A^GATCT
BsaAI 2182 CACGTA [CT]AC^GT[GA]
BsaHI 4012 GGCGCC G[GA]^CG[CT]C
BseAI 5168 TCCGGA T^CCGGA
BsmBI 2878 GAGACG CGTCTC(1/5)
BspDI 2872 ATCGAT AT^CGAT
BspEI 5168 TCCGGA T^CCGGA
BspLU11I 5256 ACATGT A^CATGT
BsrBI 5116 GAGCGG CCGCTC(-3/-3)
BsrFI 3304 ACCGGC [GA]^CCGG[CT]
BstAPI 4818 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2295 GGTCACC G^GT[ACGT]ACC
BstPI 2295 GGTCACC G^GT[ACGT]ACC
BstXI 2495 CCAGTAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3304 ACCGGC [GA]^CCGG[CT]
ClaI 2872 ATCGAT AT^CGAT
CpoI 1507 CGGTCCG CG^G[AT]CCG
DraI 159 TTTAAA TTT^AAA
DraIII 4115 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 70 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 511 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2295 GGTCACC G^GT[ACGT]ACC
EcoT22I 3289 ATGCAT ATGCA^T
Hin1I 4012 GGCGCC G[GA]^CG[CT]C
HincII 2073 GTCAAC GT[CT]^[GA]AC
HindII 2073 GTCAAC GT[CT]^[GA]AC
KasI 4012 GGCGCC G^GCGCC
KspI 953 CCGCGG CCGC^GG
MfeI 4658 CAATTG C^AATTG
MluNI 2456 TGGCCA TGG^CCA
MroI 5168 TCCGGA T^CCGGA
MscI 2456 TGGCCA TGG^CCA
MunI 4658 CAATTG C^AATTG
NarI 4012 GGCGCC GG^CGCC
NsiI 3289 ATGCAT ATGCA^T
PflFI 4783 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1507 CGGTCCG CG^G[AT]CCG
SacII 953 CCGCGG CCGC^GG
SfoI 4012 GGCGCC GGC^GCC
SmaI 2801 CCCGGG CCC^GGG
SpeI 3396 ACTAGT A^CTAGT
Tth111I 4783 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2801 CCCGGG C^CCGGG
XmaI 2801 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 2338 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3764 CAGAAACTG
Asp700I 907 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2480 GAATGCTTTC
AvrII 525 CCTAGG C^CTAGG
4393 CCTAGG
BclI 3840 TGATCA T^GATCA
4064 TGATCA
BlnI 525 CCTAGG C^CTAGG
4393 CCTAGG
Bsp1407I 2207 TGTACA T^GTACA
5146 TGTACA
BsrGI 2207 TGTACA T^GTACA
5146 TGTACA
EaeI 2456 TGGCCA [CT]^GGCC[GA]
3543 TGGCCG
EcoNI 4262 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5284 CCTGAGCCAGG
EcoRV 2869 GATATC GAT^ATC
4525 GATATC
FbaI 3840 TGATCA T^GATCA
4064 TGATCA
HaeII 4012 GGCGCC [GA]GCGC^[CT]
4587 AGCGCC
NdeI 2256 CATATG CA^TATG
3694 CATATG
PshAI 436 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2289 GACACTGGTC
SacI 1599 GAGCTC GAGCT^C
2506 GAGCTC
SspBI 2207 TGTACA T^GTACA
5146 TGTACA
SspI 176 AATATT AAT^ATT
3489 AATATT
StuI 1044 AGGCCT AGG^CCT
3295 AGGCCT
XmnI 907 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2480 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII