Restriction map for
pF1KB4486
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI AviII AvrII
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
BstBI BstUI BstXI CelII ClaI
CpoI DraI DraIII Eco47III EcoNI
FauI FbaI FspI HaeII Hin1I
HindIII HpaI KasI KspI MluI
MroI MvnI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 79 GCGG CCGC(-3/-1)
AflIII 4967 ACATGT A^C[GA][CT]GT
AhdI 306 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4864 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 306 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3785 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 218 CTCGGG C^[CT]CG[GA]G
BsaAI 821 TACGTG [CT]AC^GT[GA]
BsaBI 2866 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 4365 CTCCTC GAGGAG(10/8)
BsiEI 3937 CGGCCG CG[GA][CT]^CG
BsoBI 218 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4473 TGTACA T^GTACA
BspHI 4796 TCATGA T^CATGA
BspLU11I 4967 ACATGT A^CATGT
BspMI 2656 GCAGGT ACCTGC(4/8)
BsrGI 4473 TGTACA T^GTACA
BstAPI 70 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 4263 GCGC GCG^C
EagI 3937 CGGCCG C^GGCCG
Eam1105I 306 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3937 CGGCCG C^GGCCG
Eco52I 3937 CGGCCG C^GGCCG
EcoT22I 4801 ATGCAT ATGCA^T
FseI 3934 GGCCGGCC GGCCGG^CC
HhaI 4263 GCGC GCG^C
HinP1I 4263 GCGC G^CGC
MamI 2866 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 4801 ATGCAT ATGCA^T
PflFI 3785 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3559 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4273 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4796 TCATGA T^CATGA
SphI 4643 GCATGC GCATG^C
SspBI 4473 TGTACA T^GTACA
Tth111I 3785 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3559 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2202 TCTAGA T^CTAGA
XmnI 4864 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 71 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1163 CAGGGGCTG
BsmI 323 GAATGC GAATGC(1/-1)
1589 GCATTC
BsrDI 371 GCAATG GCAATG(2/0)
4229 GCAATG
BsrFI 28 GCCGGC [GA]^CCGG[CT]
3935 GCCGGC
Bst1107I 566 GTATAC GTA^TAC
2918 GTATAC
BstZ17I 566 GTATAC GTA^TAC
2918 GTATAC
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
3935 GCCGGC
DsaI 4302 CCGTGG C^C[GA][CT]GG
4494 CCACGG
EarI 4864 GAAGAG CTCTTC(1/4)
5046 GAAGAG
EcoRV 521 GATATC GAT^ATC
1645 GATATC
HgaI 2360 GACGC GACGC(5/10)
4226 GACGC
Ksp632I 4864 GAAGAG CTCTTC(1/4)
5046 GAAGAG
MaeII 390 ACGT A^CGT
822 ACGT
MfeI 578 CAATTG C^AATTG
749 CAATTG
MunI 578 CAATTG C^AATTG
749 CAATTG
NaeI 28 GCCGGC GCC^GGC
3935 GCCGGC
NgoMIV 28 GCCGGC G^CCGGC
3935 GCCGGC
NspI 4643 GCATGC [GA]CATG^[CT]
4967 ACATGT
SacI 1598 GAGCTC GAGCT^C
1700 GAGCTC
TaqI 4686 TCGA T^CGA
4889 TCGA
TthHB8I 4686 TCGA T^CGA
4889 TCGA
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
Acc65I AccI AcsI AfaI AluI
AlwI AocI ApaI ApoI Asp718I
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DrdI EaeI Eco81I
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
KpnI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII