Restriction map for
pF1KB3038
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI Aor51HI ApaI AscI Asp718I
AvaI AviII BbrPI BcgI BciVI
BclI BcnI BglI BsaBI BsaWI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HapII HpaII KpnI KspI MamI
MluI MroI MspI NaeI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1441 CGCG CG^CG
AflII 2929 CTTAAG C^TTAAG
AhdI 544 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4082 GTGCAC G^TGCAC
ApaLI 4082 GTGCAC G^TGCAC
AseI 2560 ATTAAT AT^TAAT
AsnI 2560 ATTAAT AT^TAAT
Asp700I 3531 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 544 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2392 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1071 CCTAGG C^CTAGG
BamHI 3036 GGATCC G^GATCC
BbeI 196 GGCGCC GGCGC^C
BbsI 3884 GTCTTC GAAGAC(2/6)
BbvCI 2332 CCTCAGC CCTCAGC(-5/-2)
BfrI 2929 CTTAAG C^TTAAG
BlnI 1071 CCTAGG C^CTAGG
BlpI 2675 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2675 GCTCAGC GC^T[ACGT]AGC
BpuAI 3884 GTCTTC GAAGAC(2/6)
BsaAI 2909 TACGTG [CT]AC^GT[GA]
BsmBI 1656 CGTCTC CGTCTC(1/5)
BsmI 384 GAATGC GAATGC(1/-1)
BspHI 186 TCATGA T^CATGA
BsrBI 90 GAGCGG CCGCTC(-3/-3)
BstAPI 334 GCATTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1441 CGCG CG^CG
BstXI 2007 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2675 GCTCAGC GC^T[ACGT]AGC
Eam1105I 544 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1565 GAATTC G^AATTC
KasI 196 GGCGCC G^GCGCC
MvnI 1441 CGCG CG^CG
NarI 196 GGCGCC GG^CGCC
NdeI 2986 CATATG CA^TATG
PflFI 2392 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2560 ATTAAT AT^TAAT
RcaI 186 TCATGA T^CATGA
SacI 2924 GAGCTC GAGCT^C
SfoI 196 GGCGCC GGC^GCC
SmlI 2929 CTTAAG C^T[CT][GA]AG
SphI 2695 GCATGC GCATG^C
SwaI 1457 ATTTAAAT ATTT^AAAT
Tth111I 2392 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2363 CCAATAACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3531 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 415 GTCTAC GT^[AC][GT]AC
1223 GTCTAC
AcyI 108 GGCGTC G[GA]^CG[CT]C
196 GGCGCC
AocI 1102 CCTCAGG CC^T[ACGT]AGG
2114 CCTCAGG
AspHI 2924 GAGCTC G[AT]GC[AT]^C
4082 GTGCAC
BanII 2589 GAGCCC G[GA]GC[CT]^C
2924 GAGCTC
BsaHI 108 GGCGTC G[GA]^CG[CT]C
196 GGCGCC
BsaI 1530 GAGACC GGTCTC(1/5)
3846 GGTCTC
BsiHKAI 2924 GAGCTC G[AT]GC[AT]^C
4082 GTGCAC
Bsp1407I 1032 TGTACA T^GTACA
2878 TGTACA
BspMI 1826 ACCTGC ACCTGC(4/8)
1954 GCAGGT
BsrGI 1032 TGTACA T^GTACA
2878 TGTACA
Bsu36I 1102 CCTCAGG CC^T[ACGT]AGG
2114 CCTCAGG
EarI 1980 CTCTTC CTCTTC(1/4)
2448 CTCTTC
Eco81I 1102 CCTCAGG CC^T[ACGT]AGG
2114 CCTCAGG
EcoT22I 431 ATGCAT ATGCA^T
754 ATGCAT
FauI 1282 CCCGC CCCGC(4/6)
79 GCGGG
HaeII 141 GGCGCT [GA]GCGC^[CT]
196 GGCGCC
HgaI 109 GCGTC GACGC(5/10)
1655 GCGTC
Hin1I 108 GGCGTC G[GA]^CG[CT]C
196 GGCGCC
HindIII 677 AAGCTT A^AGCTT
3859 AAGCTT
HpaI 2617 GTTAAC GTT^AAC
3463 GTTAAC
Ksp632I 1980 CTCTTC CTCTTC(1/4)
2448 CTCTTC
NsiI 431 ATGCAT ATGCA^T
754 ATGCAT
NspI 1356 ACATGC [GA]CATG^[CT]
2695 GCATGC
SexAI 2307 ACCAGGT A^CC[AT]GGT
3429 ACCAGGT
SspBI 1032 TGTACA T^GTACA
2878 TGTACA
SspI 3259 AATATT AAT^ATT
3472 AATATT
StuI 1016 AGGCCT AGG^CCT
3116 AGGCCT
TaqI 492 TCGA T^CGA
2231 TCGA
TthHB8I 492 TCGA T^CGA
2231 TCGA
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanI
BbvI BfaI BglII BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MunI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII