Restriction map for
pF1KB3009
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccIII AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BbeI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI Hin1I KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI PvuII RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1652 GGTACC G^GTACC
AccI 1088 GTAGAC GT^[AC][GT]AC
AccII 1350 CGCG CG^CG
AclI 711 AACGTT AA^CGTT
Asp718I 1652 GGTACC G^GTACC
AspI 1483 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 489 GGATCC G^GATCC
BbrPI 1863 CACGTG CAC^GTG
BbvCI 1681 GCTGAGG CCTCAGC(-5/-2)
BcnI 117 CCCGG CC^[GC]GG
BsaAI 1863 CACGTG [CT]AC^GT[GA]
BsmBI 442 GAGACG CGTCTC(1/5)
BspHI 1188 TCATGA T^CATGA
BsrBI 458 GAGCGG CCGCTC(-3/-3)
BsrFI 558 ACCGGC [GA]^CCGG[CT]
BstAPI 1700 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1350 CGCG CG^CG
BstXI 391 CCAAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 558 ACCGGC [GA]^CCGG[CT]
DrdI 1816 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 11 CCGTGG C^C[GA][CT]GG
EcoRI 50 GAATTC G^AATTC
HaeII 1712 GGCGCT [GA]GCGC^[CT]
HincII 1569 GTTAAC GT[CT]^[GA]AC
HindII 1569 GTTAAC GT[CT]^[GA]AC
HpaI 1569 GTTAAC GTT^AAC
KpnI 1652 GGTACC GGTAC^C
MslI 1859 CATTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1470 CCGCTG C[AC]G^C[GT]G
MvnI 1350 CGCG CG^CG
NciI 117 CCCGG CC^[GC]GG
PflFI 1483 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 331 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1863 CACGTG CAC^GTG
PmlI 1863 CACGTG CAC^GTG
Psp1406I 711 AACGTT AA^CGTT
RcaI 1188 TCATGA T^CATGA
SbfI 600 CCTGCAGG CCTGCA^GG
Sse8387I 600 CCTGCAGG CCTGCA^GG
SspI 1117 AATATT AAT^ATT
StuI 1018 AGGCCT AGG^CCT
TaqI 505 TCGA T^CGA
TfiI 1586 GAATC G^A[AT]TC
Tth111I 1483 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 505 TCGA T^CGA
Van91I 331 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 589 GAGCTC G[AT]GC[AT]^C
654 GAGCTC
AvaI 511 CCCGAG C^[CT]CG[GA]G
625 CCCGAG
BbsI 805 GAAGAC GAAGAC(2/6)
1668 GAAGAC
BfaI 179 CTAG C^TAG
256 CTAG
BpuAI 805 GAAGAC GAAGAC(2/6)
1668 GAAGAC
BsaWI 1373 ACCGGA [AT]^CCGG[AT]
1664 ACCGGA
BsiHKAI 589 GAGCTC G[AT]GC[AT]^C
654 GAGCTC
BsoBI 511 CCCGAG C^[CT]CG[GA]G
625 CCCGAG
BstF5I 111 GGATG GGATG(2/0)
720 GGATG
EcoNI 432 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1763 CCTCAGTGAGG
EcoRV 895 GATATC GAT^ATC
1898 GATATC
EcoT14I 391 CCAAGG C^C[AT][AT]GG
883 CCTTGG
FauI 318 GCGGG CCCGC(4/6)
1709 GCGGG
FokI 111 GGATG GGATG(9/13)
720 GGATG
HindIII 1548 AAGCTT A^AGCTT
1884 AAGCTT
MaeI 179 CTAG C^TAG
256 CTAG
MaeII 712 ACGT A^CGT
1864 ACGT
NlaIII 412 CATG CATG^
1189 CATG
SacI 589 GAGCTC GAGCT^C
654 GAGCTC
StyI 391 CCAAGG C^C[AT][AT]GG
883 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AvaII
BanI BanII BbvI BglII BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIV PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI