Restriction map for
pF1KB0063
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AscI Asp718I AspI AviII
AvrII BbeI BbrPI BciVI BfrI
BglII BlnI BsaBI BsiWI BspHI
BsrBI BssHII Bst1107I BstZ17I CpoI
DraI Eco47III EcoT22I FseI FspI
KasI KpnI MamI MluI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PflFI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SalI SbfI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 2022 GACGTC GACGT^C
AcyI 2022 GACGTC G[GA]^CG[CT]C
AgeI 5743 ACCGGT A^CCGGT
AseI 4618 ATTAAT AT^TAAT
AsnI 4618 ATTAAT AT^TAAT
Asp700I 253 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 2459 TGATCA T^GATCA
BlpI 366 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 366 GCTGAGC GC^T[ACGT]AGC
BsaAI 2127 CACGTA [CT]AC^GT[GA]
BsaHI 2022 GACGTC G[GA]^CG[CT]C
Bsp1407I 1964 TGTACA T^GTACA
BsrGI 1964 TGTACA T^GTACA
BstBI 715 TTCGAA TT^CGAA
CelII 366 GCTGAGC GC^T[ACGT]AGC
DraIII 2350 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5479 GACAGTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1431 CGGCCG C^GGCCG
EclXI 1431 CGGCCG C^GGCCG
Eco52I 1431 CGGCCG C^GGCCG
EcoRV 2340 GATATC GAT^ATC
FbaI 2459 TGATCA T^GATCA
Hin1I 2022 GACGTC G[GA]^CG[CT]C
HindIII 255 AAGCTT A^AGCTT
HpaI 6499 GTTAAC GTT^AAC
KspI 3705 CCGCGG CCGC^GG
NdeI 6125 CATATG CA^TATG
NspV 715 TTCGAA TT^CGAA
PinAI 5743 ACCGGT A^CCGGT
PshAI 3647 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4618 ATTAAT AT^TAAT
SacII 3705 CCGCGG CCGC^GG
SfiI 5401 GGCCCATCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 715 TTCGAA TT^CGAA
SspBI 1964 TGTACA T^GTACA
XbaI 2202 TCTAGA T^CTAGA
XmnI 253 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 1162 GTAGAC GT^[AC][GT]AC
4516 GTCTAC
AccII 3706 CGCG CG^CG
6097 CGCG
BamHI 181 GGATCC G^GATCC
2196 GGATCC
BsmI 4659 GAATGC GAATGC(1/-1)
2102 GCATTC
BspDI 2488 ATCGAT AT^CGAT
2545 ATCGAT
BssSI 1741 CACGAG CACGAG(-5/-1)
1978 CACGAG
BstAPI 1664 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6124 GCATATGCTGC
BstEII 2391 GGTGACC G^GT[ACGT]ACC
5746 GGTTACC
BstPI 2391 GGTGACC G^GT[ACGT]ACC
5746 GGTTACC
BstUI 3706 CGCG CG^CG
6097 CGCG
ClaI 2488 ATCGAT AT^CGAT
2545 ATCGAT
EcoO65I 2391 GGTGACC G^GT[ACGT]ACC
5746 GGTTACC
EcoRI 4720 GAATTC G^AATTC
6157 GAATTC
HaeII 2113 AGCGCC [GA]GCGC^[CT]
6024 GGCGCT
MfeI 858 CAATTG C^AATTG
5918 CAATTG
MunI 858 CAATTG C^AATTG
5918 CAATTG
MvnI 3706 CGCG CG^CG
6097 CGCG
PaeR7I 4219 CTCGAG C^TCGAG
5569 CTCGAG
PflMI 135 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3072 CCAGTGGGTGG
PpuMI 68 GGGTCCT [GA]G^G[AT]CC[CT]
2966 AGGACCT
SapI 2097 GAAGAGC GCTCTTC(1/4)
5622 GAAGAGC
Van91I 135 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3072 CCAGTGGGTGG
XhoI 4219 CTCGAG C^TCGAG
5569 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccIII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI