Restriction map for
pF1KA2021
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 586 GTCTAC GT^[AC][GT]AC
AccIII 126 TCCGGA T^CCGGA
Alw44I 256 GTGCAC G^TGCAC
Aor51HI 1109 AGCGCT AGC^GCT
ApaI 1724 GGGCCC GGGCC^C
ApaLI 256 GTGCAC G^TGCAC
AvaI 226 CCCGAG C^[CT]CG[GA]G
AviII 398 TGCGCA TGC^GCA
BalI 1868 TGGCCA TGG^CCA
BbvCI 562 GCTGAGG CCTCAGC(-5/-2)
BglI 1717 GCCTTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 126 TCCGGA [AT]^CCGG[AT]
BseAI 126 TCCGGA T^CCGGA
BsmI 1285 GCATTC GAATGC(1/-1)
BsoBI 226 CCCGAG C^[CT]CG[GA]G
BspEI 126 TCCGGA T^CCGGA
BspMI 999 GCAGGT ACCTGC(4/8)
BstBI 1508 TTCGAA TT^CGAA
Eco47III 1109 AGCGCT AGC^GCT
FspI 398 TGCGCA TGC^GCA
HgaI 1608 GACGC GACGC(5/10)
HphI 414 TCACC GGTGA(8/7)
MluNI 1868 TGGCCA TGG^CCA
MroI 126 TCCGGA T^CCGGA
MscI 1868 TGGCCA TGG^CCA
NaeI 144 GCCGGC GCC^GGC
NcoI 1677 CCATGG C^CATGG
NgoMIV 144 GCCGGC G^CCGGC
NspV 1508 TTCGAA TT^CGAA
SacI 780 GAGCTC GAGCT^C
ScaI 922 AGTACT AGT^ACT
SexAI 1406 ACCAGGT A^CC[AT]GGT
SfuI 1508 TTCGAA TT^CGAA
SmlI 1719 CTTGAG C^T[CT][GA]AG
Tsp509I 1737 AATT ^AATT
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 35 GGCGCC G[GA]^CG[CT]C
86 GGCGCC
BbeI 35 GGCGCC GGCGC^C
86 GGCGCC
BglII 246 AGATCT A^GATCT
824 AGATCT
BlpI 1164 GCTGAGC GC^T[ACGT]AGC
1744 GCTGAGC
Bpu1102I 1164 GCTGAGC GC^T[ACGT]AGC
1744 GCTGAGC
BsaHI 35 GGCGCC G[GA]^CG[CT]C
86 GGCGCC
BsrFI 38 GCCGGT [GA]^CCGG[CT]
144 GCCGGC
BstYI 246 AGATCT [GA]^GATC[CT]
824 AGATCT
CelII 1164 GCTGAGC GC^T[ACGT]AGC
1744 GCTGAGC
Cfr10I 38 GCCGGT [GA]^CCGG[CT]
144 GCCGGC
EcoNI 993 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1038 CCTCTTTGAGG
Hin1I 35 GGCGCC G[GA]^CG[CT]C
86 GGCGCC
KasI 35 GGCGCC G^GCGCC
86 GGCGCC
MflI 246 AGATCT [GA]^GATC[CT]
824 AGATCT
MslI 801 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1341 CACTTTGATG
NarI 35 GGCGCC GG^CGCC
86 GGCGCC
PflMI 46 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1791 CCACCATGTGG
SfoI 35 GGCGCC GGC^GCC
86 GGCGCC
Van91I 46 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1791 CCACCATGTGG
XcmI 524 CCACGGAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1554 CCAAAGTCCCGCTGG
XhoII 246 AGATCT [GA]^GATC[CT]
824 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I