Restriction map for
pF1KA2009
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
MfeI MflI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 498 GGTACC G^GTACC
Alw44I 1282 GTGCAC G^TGCAC
Aor51HI 270 AGCGCT AGC^GCT
ApaLI 1282 GTGCAC G^TGCAC
Asp700I 714 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 498 GGTACC G^GTACC
BcgI 1572 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1342 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 573 CACGTA [CT]AC^GT[GA]
BsaBI 751 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 76 GAGACC GGTCTC(1/5)
BsaWI 1170 TCCGGT [AT]^CCGG[AT]
BsgI 1294 CTGCAC GTGCAG(16/14)
BsiEI 828 CGGCCG CG[GA][CT]^CG
BssSI 520 CTCGTG CACGAG(-5/-1)
BstEII 618 GGTGACC G^GT[ACGT]ACC
BstPI 618 GGTGACC G^GT[ACGT]ACC
DraII 528 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 828 CGGCCG C^GGCCG
EclXI 828 CGGCCG C^GGCCG
Eco47III 270 AGCGCT AGC^GCT
Eco52I 828 CGGCCG C^GGCCG
EcoO109I 528 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 618 GGTGACC G^GT[ACGT]ACC
EcoT14I 362 CCATGG C^C[AT][AT]GG
HinfI 892 GACTC G^A[ACGT]TC
HphI 618 GGTGA GGTGA(8/7)
KpnI 498 GGTACC GGTAC^C
KspI 1676 CCGCGG CCGC^GG
MamI 751 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1289 GCCGGC GCC^GGC
NcoI 362 CCATGG C^CATGG
NgoMIV 1289 GCCGGC G^CCGGC
PaeR7I 650 CTCGAG C^TCGAG
PflMI 936 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 892 GACTC GAGTC(4/5)
SacI 700 GAGCTC GAGCT^C
SacII 1676 CCGCGG CCGC^GG
SmlI 650 CTCGAG C^T[CT][GA]AG
SphI 626 GCATGC GCATG^C
StuI 1401 AGGCCT AGG^CCT
StyI 362 CCATGG C^C[AT][AT]GG
Van91I 936 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 650 CTCGAG C^TCGAG
XmnI 714 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 572 ACACGT A^C[GA][CT]GT
1466 ACACGT
AlwI 408 GATCC GGATC(4/5)
642 GGATC
AlwNI 786 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
937 CAGCGCCTG
ApaI 529 GGGCCC GGGCC^C
1130 GGGCCC
AviII 981 TGCGCA TGC^GCA
1379 TGCGCA
BalI 359 TGGCCA TGG^CCA
1599 TGGCCA
BbsI 282 GAAGAC GAAGAC(2/6)
1228 GTCTTC
BfaI 845 CTAG C^TAG
962 CTAG
BpuAI 282 GAAGAC GAAGAC(2/6)
1228 GTCTTC
BsmAI 76 GAGAC GTCTC(1/5)
1323 GTCTC
BsmFI 910 GGGAC GGGAC(10/14)
1146 GTCCC
BspMI 467 ACCTGC ACCTGC(4/8)
1161 ACCTGC
BsrBI 1100 CCGCTC CCGCTC(-3/-3)
1141 GAGCGG
BsrDI 672 CATTGC GCAATG(2/0)
1010 GCAATG
BstAPI 1304 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1616 GCATGGAGTGC
BstXI 1120 CCACTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1424 CCAACGGGCTGG
FspI 981 TGCGCA TGC^GCA
1379 TGCGCA
HgaI 567 GACGC GACGC(5/10)
585 GACGC
MaeI 845 CTAG C^TAG
962 CTAG
MluNI 359 TGGCCA TGG^CCA
1599 TGGCCA
MscI 359 TGGCCA TGG^CCA
1599 TGGCCA
MslI 486 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
735 CACCGATGTG
NspI 228 GCATGT [GA]CATG^[CT]
626 GCATGC
PvuII 1308 CAGCTG CAG^CTG
1441 CAGCTG
XcmI 781 CCAGGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1259 CCAACGCCAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI