Restriction map for
pF1KA1998
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
AflII AgeI AocI AscI AvrII
BbrPI BciVI BfrI BlnI BlpI
Bpu1102I BsiWI Bsp1407I BsrGI BssHII
Bst1107I BstZ17I Bsu36I CelII CpoI
Eco81I FseI MfeI MluI MunI
NdeI NheI NotI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SgrAI SnaBI Sse8387I
SspBI SwaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2281 GGTACC G^GTACC
AccIII 803 TCCGGA T^CCGGA
AclI 157 AACGTT AA^CGTT
Aor51HI 3087 AGCGCT AGC^GCT
ApaI 4565 GGGCCC GGGCC^C
AseI 1171 ATTAAT AT^TAAT
AsnI 1171 ATTAAT AT^TAAT
Asp718I 2281 GGTACC G^GTACC
AspI 3916 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4364 TGCGCA TGC^GCA
BbeI 3880 GGCGCC GGCGC^C
BglII 2618 AGATCT A^GATCT
BseAI 803 TCCGGA T^CCGGA
BspDI 4661 ATCGAT AT^CGAT
BspEI 803 TCCGGA T^CCGGA
BssSI 406 CTCGTG CACGAG(-5/-1)
BstBI 4857 TTCGAA TT^CGAA
ClaI 4661 ATCGAT AT^CGAT
DrdI 4045 GACTTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4599 CGGCCG C^GGCCG
EclXI 4599 CGGCCG C^GGCCG
Eco47III 3087 AGCGCT AGC^GCT
Eco52I 4599 CGGCCG C^GGCCG
EcoNI 3784 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 4364 TGCGCA TGC^GCA
HincII 2884 GTTAAC GT[CT]^[GA]AC
HindII 2884 GTTAAC GT[CT]^[GA]AC
HpaI 2884 GTTAAC GTT^AAC
KasI 3880 GGCGCC G^GCGCC
KpnI 2281 GGTACC GGTAC^C
KspI 4619 CCGCGG CCGC^GG
MroI 803 TCCGGA T^CCGGA
NarI 3880 GGCGCC GG^CGCC
NruI 4281 TCGCGA TCG^CGA
NspV 4857 TTCGAA TT^CGAA
PacI 3097 TTAATTAA TTAAT^TAA
PflFI 3916 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1171 ATTAAT AT^TAAT
Psp1406I 157 AACGTT AA^CGTT
SacII 4619 CCGCGG CCGC^GG
SfoI 3880 GGCGCC GGC^GCC
SfuI 4857 TTCGAA TT^CGAA
SpeI 4813 ACTAGT A^CTAGT
SphI 2092 GCATGC GCATG^C
SspI 5101 AATATT AAT^ATT
Tth111I 3916 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AatII 649 GACGTC GACGT^C
3751 GACGTC
AccI 1117 GTCTAC GT^[AC][GT]AC
4828 GTAGAC
AflIII 126 ACATGT A^C[GA][CT]GT
3216 ACATGT
AhdI 2549 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4270 GACCAGAAGTC
Alw44I 2062 GTGCAC G^TGCAC
4318 GTGCAC
ApaLI 2062 GTGCAC G^TGCAC
4318 GTGCAC
AspEI 2549 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4270 GACCAGAAGTC
BalI 3635 TGGCCA TGG^CCA
4310 TGGCCA
BbvCI 2454 CCTCAGC CCTCAGC(-5/-2)
4518 GCTGAGG
BcgI 588 CGAAGAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
315 GCAGGCCAATCG
BsaAI 271 TACGTG [CT]AC^GT[GA]
620 TACGTG
BsgI 694 GTGCAG GTGCAG(16/14)
893 GTGCAG
BsiEI 2784 CGACCG CG[GA][CT]^CG
4599 CGGCCG
BspLU11I 126 ACATGT A^CATGT
3216 ACATGT
BsrDI 1067 CATTGC GCAATG(2/0)
3648 GCAATG
BsrFI 1082 GCCGGC [GA]^CCGG[CT]
4601 GCCGGC
BstAPI 382 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4572 GCAGAGCCTGC
BstEII 243 GGTGACC G^GT[ACGT]ACC
4038 GGTAACC
BstPI 243 GGTGACC G^GT[ACGT]ACC
4038 GGTAACC
BstXI 635 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2312 CCACATCCTTGG
Cfr10I 1082 GCCGGC [GA]^CCGG[CT]
4601 GCCGGC
DraI 2893 TTTAAA TTT^AAA
3126 TTTAAA
DraII 518 GGGGCCT [GA]G^G[ACGT]CC[CT]
4223 AGGGCCT
DraIII 1221 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1283 CACTTAGTG
Eam1105I 2549 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4270 GACCAGAAGTC
EcoO109I 518 GGGGCCT [GA]G^G[ACGT]CC[CT]
4223 AGGGCCT
EcoO65I 243 GGTGACC G^GT[ACGT]ACC
4038 GGTAACC
EcoT22I 2584 ATGCAT ATGCA^T
2982 ATGCAT
MluNI 3635 TGGCCA TGG^CCA
4310 TGGCCA
MscI 3635 TGGCCA TGG^CCA
4310 TGGCCA
NaeI 1082 GCCGGC GCC^GGC
4601 GCCGGC
NgoMIV 1082 GCCGGC G^CCGGC
4601 GCCGGC
NsiI 2584 ATGCAT ATGCA^T
2982 ATGCAT
PvuII 2343 CAGCTG CAG^CTG
2650 CAGCTG
SacI 403 GAGCTC GAGCT^C
1512 GAGCTC
SapI 1744 GAAGAGC GCTCTTC(1/4)
2390 GCTCTTC
SmaI 555 CCCGGG CCC^GGG
4021 CCCGGG
XbaI 438 TCTAGA T^CTAGA
3759 TCTAGA
XmaCI 555 CCCGGG C^CCGGG
4021 CCCGGG
XmaI 555 CCCGGG C^CCGGG
4021 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRI EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI