Restriction map for
pF1KA1983
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwI
AlwNI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MflI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 156 CGCG CG^CG
Alw44I 303 GTGCAC G^TGCAC
AocI 1111 CCTCAGG CC^T[ACGT]AGG
ApaLI 303 GTGCAC G^TGCAC
BalI 552 TGGCCA TGG^CCA
BbrPI 93 CACGTG CAC^GTG
BcgI 270 CGACGTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 346 GGATAC GTATCC(6/5)
BsaAI 93 CACGTG [CT]AC^GT[GA]
BsaBI 1159 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 980 GGTCTC GGTCTC(1/5)
BspLU11I 517 ACATGT A^CATGT
BstAPI 627 GCAGACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 156 CGCG CG^CG
BstXI 1139 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1111 CCTCAGG CC^T[ACGT]AGG
CfoI 964 GCGC GCG^C
DpnII 877 GATC ^GATC
Eco81I 1111 CCTCAGG CC^T[ACGT]AGG
EcoT22I 252 ATGCAT ATGCA^T
FauI 378 GCGGG CCCGC(4/6)
HaeII 963 AGCGCC [GA]GCGC^[CT]
HgaI 429 GACGC GACGC(5/10)
HhaI 964 GCGC GCG^C
HinP1I 964 GCGC G^CGC
HinfI 1080 GACTC G^A[ACGT]TC
MaeIII 704 GTGAC ^GT[ACGT]AC
MamI 1159 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 877 GATC ^GATC
MluNI 552 TGGCCA TGG^CCA
MscI 552 TGGCCA TGG^CCA
MseI 890 TTAA T^TAA
MvnI 156 CGCG CG^CG
NdeII 877 GATC ^GATC
NruI 155 TCGCGA TCG^CGA
NsiI 252 ATGCAT ATGCA^T
PleI 1080 GACTC GAGTC(4/5)
PmaCI 93 CACGTG CAC^GTG
PmlI 93 CACGTG CAC^GTG
SacI 193 GAGCTC GAGCT^C
Sau3AI 877 GATC ^GATC
SfiI 898 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1191 CTTGAG C^T[CT][GA]AG
TaqI 1004 TCGA T^CGA
Tru9I 890 TTAA T^TAA
Tsp45I 704 GTGAC ^GT[GC]AC
TthHB8I 1004 TCGA T^CGA
XcmI 35 CCAGGGGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 235 AAATTT [GA]^AATT[CT]
1117 GAATTT
AfaI 332 GTAC GT^AC
521 GTAC
AflIII 92 ACACGT A^C[GA][CT]GT
517 ACATGT
ApoI 235 AAATTT [GA]^AATT[CT]
1117 GAATTT
AspHI 193 GAGCTC G[AT]GC[AT]^C
303 GTGCAC
BbsI 513 GAAGAC GAAGAC(2/6)
183 GTCTTC
BpmI 983 CTCCAG CTGGAG(16/14)
1154 CTGGAG
BpuAI 513 GAAGAC GAAGAC(2/6)
183 GTCTTC
BsaWI 371 ACCGGA [AT]^CCGG[AT]
1076 ACCGGA
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
303 GTGCAC
BsrDI 422 GCAATG GCAATG(2/0)
1037 GCAATG
BsrI 550 ACTGG ACTGG(1/-1)
700 ACTGG
EaeI 318 TGGCCG [CT]^GGCC[GA]
552 TGGCCA
EarI 1086 CTCTTC CTCTTC(1/4)
935 GAAGAG
Ksp632I 1086 CTCTTC CTCTTC(1/4)
935 GAAGAG
MaeII 94 ACGT A^CGT
272 ACGT
NcoI 860 CCATGG C^CATGG
1139 CCATGG
PstI 637 CTGCAG CTGCA^G
1054 CTGCAG
RsaI 332 GTAC GT^AC
521 GTAC
SfaNI 254 GCATC GCATC(5/9)
449 GCATC
Tsp509I 236 AATT ^AATT
1118 AATT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI ApaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PpuMI PvuII Sau96I ScrFI SfcI
StyI TseI TspRI