Restriction map for
pF1KA1966
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# NO CUT ENZYMES ( 136 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HhaI HinP1I
HindIII HpaI KasI KpnI KspI
MluI MluNI MscI MspA1I MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1562 GACGTC GACGT^C
AccIII 404 TCCGGA T^CCGGA
Asp700I 1535 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 222 GAGCTC G[AT]GC[AT]^C
BanII 222 GAGCTC G[GA]GC[CT]^C
BbvI 557 GCAGC GCAGC(8/12)
BmyI 222 GAGCTC G[AGT]GC[ACT]^C
BpmI 363 CTCCAG CTGGAG(16/14)
BsaBI 328 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 404 TCCGGA [AT]^CCGG[AT]
BseAI 404 TCCGGA T^CCGGA
BsiHKAI 222 GAGCTC G[AT]GC[AT]^C
BsmFI 1797 GGGAC GGGAC(10/14)
Bsp1286I 222 GAGCTC G[AGT]GC[ACT]^C
BspEI 404 TCCGGA T^CCGGA
BspMI 970 GCAGGT ACCTGC(4/8)
BssSI 1556 CACGAG CACGAG(-5/-1)
BstAPI 1815 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 1759 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1279 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2016 TGGCCG [CT]^GGCC[GA]
EcoO109I 1759 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1537 ATGCAT ATGCA^T
HincII 1604 GTCGAC GT[CT]^[GA]AC
HindII 1604 GTCGAC GT[CT]^[GA]AC
MamI 328 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 926 CAATTG C^AATTG
MroI 404 TCCGGA T^CCGGA
MunI 926 CAATTG C^AATTG
NdeI 965 CATATG CA^TATG
NsiI 1537 ATGCAT ATGCA^T
PpuMI 1759 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 222 GAGCTC GAGCT^C
SalI 1604 GTCGAC G^TCGAC
SmlI 1889 CTCAAG C^T[CT][GA]AG
SspI 1197 AATATT AAT^ATT
TseI 557 GCAGC G^C[AT]GC
XbaI 324 TCTAGA T^CTAGA
XcmI 1801 CCACCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1535 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 1604 GTCGAC GT^[AC][GT]AC
2027 GTAGAC
AciI 2064 CCGC CCGC(-3/-1)
4 GCGG
AcyI 1562 GACGTC G[GA]^CG[CT]C
1601 GGCGTC
BamHI 414 GGATCC G^GATCC
1695 GGATCC
BbsI 504 GTCTTC GAAGAC(2/6)
1466 GTCTTC
BcnI 1587 CCGGG CC^[GC]GG
2046 CCGGG
BpuAI 504 GTCTTC GAAGAC(2/6)
1466 GTCTTC
BsaHI 1562 GACGTC G[GA]^CG[CT]C
1601 GGCGTC
BstXI 546 CCATGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1804 CCACCACCTTGG
Cac8I 487 GCAAGC GC[ACGT]^[ACGT]GC
1346 GCAGGC
Fnu4HI 4 GCGGC GC^[ACGT]GC
557 GCAGC
Hin1I 1562 GACGTC G[GA]^CG[CT]C
1601 GGCGTC
HphI 1382 TCACC GGTGA(8/7)
1671 TCACC
ItaI 4 GCGGC GC^[ACGT]GC
557 GCAGC
MslI 1093 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1960 CATGATTATG
NciI 1587 CCGGG CC^[GC]GG
2046 CCGGG
PflMI 1385 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1707 CCAGGAAGTGG
PleI 814 GACTC GAGTC(4/5)
961 GAGTC
Tsp45I 812 GTGAC ^GT[GC]AC
1523 GTCAC
Van91I 1385 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1707 CCAGGAAGTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AvaII BfaI BglII BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII EcoT14I FokI HaeIII
HapII HgaI HinfI HpaII Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XhoII