Restriction map for
pF1KA1948
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BcnI BfaI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII ItaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NlaIV NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TseI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AflII 334 CTTAAG C^TTAAG
BbsI 215 GAAGAC GAAGAC(2/6)
BfrI 334 CTTAAG C^TTAAG
BglII 717 AGATCT A^GATCT
BpmI 47 CTCCAG CTGGAG(16/14)
BpuAI 215 GAAGAC GAAGAC(2/6)
Bsp1407I 89 TGTACA T^GTACA
BsrGI 89 TGTACA T^GTACA
BssKI 66 CCTGG ^CC[ACGT]GG
BstNI 66 CCTGG CC^[AT]GG
BstXI 49 CCAGAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 717 AGATCT [GA]^GATC[CT]
Cfr13I 3 GGCCC G^G[ACGT]CC
DraI 348 TTTAAA TTT^AAA
EarI 52 GAAGAG CTCTTC(1/4)
EcoNI 397 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 66 CCTGG ^CC[AT]GG
EcoT22I 739 ATGCAT ATGCA^T
Ksp632I 52 GAAGAG CTCTTC(1/4)
MaeIII 469 GTTAC ^GT[ACGT]AC
MflI 717 AGATCT [GA]^GATC[CT]
MvaI 66 CCTGG CC^[AT]GG
NcoI 327 CCATGG C^CATGG
NdeI 705 CATATG CA^TATG
NsiI 739 ATGCAT ATGCA^T
PleI 891 GACTC GAGTC(4/5)
Sau96I 3 GGCCC G^G[ACGT]CC
ScrFI 66 CCTGG CC^[ACGT]GG
SfcI 114 CTACAG C^T[GA][CT]AG
SspBI 89 TGTACA T^GTACA
XhoII 717 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 1028 GCGG CCGC(-3/-1)
1280 GCGG
BciVI 406 GGATAC GTATCC(6/5)
31 GTATCC
BsgI 629 CTGCAC GTGCAG(16/14)
713 GTGCAG
BsiYI 66 CCTGGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
397 CCTCAAGAAGG
BslI 66 CCTGGACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
397 CCTCAAGAAGG
CfoI 543 GCGC GCG^C
1383 GCGC
DpnII 11 GATC ^GATC
718 GATC
DsaI 327 CCATGG C^C[GA][CT]GG
1128 CCGTGG
EcoT14I 190 CCTTGG C^C[AT][AT]GG
327 CCATGG
FauI 1028 GCGGG CCCGC(4/6)
1280 GCGGG
HhaI 543 GCGC GCG^C
1383 GCGC
HinP1I 543 GCGC G^CGC
1383 GCGC
HindIII 698 AAGCTT A^AGCTT
904 AAGCTT
MboI 11 GATC ^GATC
718 GATC
MwoI 543 GCGCCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
897 GCATCAGAAGC
NdeII 11 GATC ^GATC
718 GATC
NlaIII 102 CATG CATG^
328 CATG
Sau3AI 11 GATC ^GATC
718 GATC
SmlI 334 CTTAAG C^T[CT][GA]AG
398 CTCAAG
StyI 190 CCTTGG C^C[AT][AT]GG
327 CCATGG
TaqI 9 TCGA T^CGA
642 TCGA
TfiI 364 GAATC G^A[AT]TC
822 GATTC
TthHB8I 9 TCGA T^CGA
642 TCGA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
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AcsI AfaI AluI ApoI Asp700I
BsaI BsaJI BsmAI BsmI BsrI
BstF5I DdeI EcoRI FokI HaeIII
HinfI HphI MboII MnlI MseI
MslI RsaI SfaNI StuI Tru9I
Tsp509I TspRI XmnI