Restriction map for
pF1KA1880
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AccII AccIII AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII KpnI KspI MflI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 109 GACGTC GACGT^C
AclI 134 AACGTT AA^CGTT
AcsI 230 AAATTC [GA]^AATT[CT]
AfaI 1434 GTAC GT^AC
AflIII 1384 ACATGT A^C[GA][CT]GT
Alw44I 55 GTGCAC G^TGCAC
AlwI 915 GGATC GGATC(4/5)
ApaLI 55 GTGCAC G^TGCAC
ApoI 230 AAATTC [GA]^AATT[CT]
AspHI 55 GTGCAC G[AT]GC[AT]^C
BanII 848 GAGCCC G[GA]GC[CT]^C
BbeI 1767 GGCGCC GGCGC^C
BcgI 1749 CGACTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1007 GGATAC GTATCC(6/5)
BclI 48 TGATCA T^GATCA
BcnI 780 CCGGG CC^[GC]GG
BglI 953 GCCGTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1791 CTCCAG CTGGAG(16/14)
BsaBI 910 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 319 GAGGAG GAGGAG(10/8)
BsgI 399 CTGCAC GTGCAG(16/14)
BsiHKAI 55 GTGCAC G[AT]GC[AT]^C
BspHI 686 TCATGA T^CATGA
BspLU11I 1384 ACATGT A^CATGT
BspMI 1151 GCAGGT ACCTGC(4/8)
BsrDI 828 CATTGC GCAATG(2/0)
BstBI 1160 TTCGAA TT^CGAA
DraI 558 TTTAAA TTT^AAA
DraIII 1201 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1733 TGGCCG [CT]^GGCC[GA]
EarI 17 CTCTTC CTCTTC(1/4)
FbaI 48 TGATCA T^GATCA
HaeII 1767 GGCGCC [GA]GCGC^[CT]
HincII 468 GTTAAC GT[CT]^[GA]AC
HindII 468 GTTAAC GT[CT]^[GA]AC
HpaI 468 GTTAAC GTT^AAC
KasI 1767 GGCGCC G^GCGCC
Ksp632I 17 CTCTTC CTCTTC(1/4)
MamI 910 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1767 GGCGCC GG^CGCC
NciI 780 CCGGG CC^[GC]GG
NspV 1160 TTCGAA TT^CGAA
PshAI 728 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 134 AACGTT AA^CGTT
RcaI 686 TCATGA T^CATGA
RsaI 1434 GTAC GT^AC
SfoI 1767 GGCGCC GGC^GCC
SfuI 1160 TTCGAA TT^CGAA
SphI 806 GCATGC GCATG^C
SspI 176 AATATT AAT^ATT
StuI 718 AGGCCT AGG^CCT
XcmI 1392 CCAGCTAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 294 GCGG CCGC(-3/-1)
1764 GCGG
AcyI 109 GACGTC G[GA]^CG[CT]C
1767 GGCGCC
BmyI 55 GTGCAC G[AGT]GC[ACT]^C
848 GAGCCC
BsaHI 109 GACGTC G[GA]^CG[CT]C
1767 GGCGCC
Bsp1286I 55 GTGCAC G[AGT]GC[ACT]^C
848 GAGCCC
BstAPI 666 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1282 GCAACAATTGC
CfoI 1759 GCGC GCG^C
1768 GCGC
DsaI 1268 CCATGG C^C[GA][CT]GG
1529 CCATGG
EcoT14I 1268 CCATGG C^C[AT][AT]GG
1529 CCATGG
FauI 294 GCGGG CCCGC(4/6)
1764 GCGGG
HaeIII 719 GGCC GG^CC
1734 GGCC
HhaI 1759 GCGC GCG^C
1768 GCGC
Hin1I 109 GACGTC G[GA]^CG[CT]C
1767 GGCGCC
HinP1I 1759 GCGC G^CGC
1768 GCGC
HphI 892 GGTGA GGTGA(8/7)
1389 TCACC
MfeI 938 CAATTG C^AATTG
1286 CAATTG
MunI 938 CAATTG C^AATTG
1286 CAATTG
NcoI 1268 CCATGG C^CATGG
1529 CCATGG
SmlI 519 CTCAAG C^T[CT][GA]AG
1246 CTTGAG
StyI 1268 CCATGG C^C[AT][AT]GG
1529 CCATGG
Tsp45I 112 GTCAC ^GT[GC]AC
1388 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AluI AvaII BanI BbvI BfaI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI BsrFI BsrI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HapII
HinfI HpaII ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I