Restriction map for
pF1KA1782
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AhdI AlwNI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmBI BsmI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MluI MroI MspA1I MvnI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 343 GACGTC GACGT^C
AcsI 1573 GAATTC [GA]^AATT[CT]
AcyI 343 GACGTC G[GA]^CG[CT]C
AgeI 1565 ACCGGT A^CCGGT
Alw44I 385 GTGCAC G^TGCAC
ApaLI 385 GTGCAC G^TGCAC
ApoI 1573 GAATTC [GA]^AATT[CT]
AspI 1480 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1108 GGATCC G^GATCC
BbsI 10 GAAGAC GAAGAC(2/6)
BcnI 1593 CCGGG CC^[GC]GG
BpuAI 10 GAAGAC GAAGAC(2/6)
BsaAI 734 TACGTG [CT]AC^GT[GA]
BsaHI 343 GACGTC G[GA]^CG[CT]C
BsaI 1523 GAGACC GGTCTC(1/5)
BsaWI 1565 ACCGGT [AT]^CCGG[AT]
BsiEI 798 CGATCG CG[GA][CT]^CG
Bsp1407I 167 TGTACA T^GTACA
BspDI 796 ATCGAT AT^CGAT
BsrGI 167 TGTACA T^GTACA
BstAPI 1208 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 553 GGTCACC G^GT[ACGT]ACC
BstPI 553 GGTCACC G^GT[ACGT]ACC
BstYI 1108 GGATCC [GA]^GATC[CT]
ClaI 796 ATCGAT AT^CGAT
DraI 502 TTTAAA TTT^AAA
DraIII 137 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1512 CCATGG C^C[GA][CT]GG
EcoO65I 553 GGTCACC G^GT[ACGT]ACC
EcoRI 1573 GAATTC G^AATTC
HgaI 1002 GCGTC GACGC(5/10)
Hin1I 343 GACGTC G[GA]^CG[CT]C
HincII 1091 GTCGAC GT[CT]^[GA]AC
HindII 1091 GTCGAC GT[CT]^[GA]AC
HindIII 704 AAGCTT A^AGCTT
MfeI 1026 CAATTG C^AATTG
MflI 1108 GGATCC [GA]^GATC[CT]
MseI 503 TTAA T^TAA
MunI 1026 CAATTG C^AATTG
NciI 1593 CCGGG CC^[GC]GG
NcoI 1512 CCATGG C^CATGG
PflFI 1480 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 702 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1565 ACCGGT A^CCGGT
PvuI 798 CGATCG CGAT^CG
SalI 1091 GTCGAC G^TCGAC
SexAI 720 ACCAGGT A^CC[AT]GGT
SfcI 679 CTACAG C^T[GA][CT]AG
SgrAI 532 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 334 CTCAAG C^T[CT][GA]AG
SspBI 167 TGTACA T^GTACA
StuI 1442 AGGCCT AGG^CCT
TfiI 247 GATTC G^A[AT]TC
Tru9I 503 TTAA T^TAA
Tth111I 1480 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 702 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1108 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1063 GTCTAC GT^[AC][GT]AC
1091 GTCGAC
AlwI 1109 GATCC GGATC(4/5)
1108 GGATC
AocI 1132 CCTGAGG CC^T[ACGT]AGG
1279 CCTCAGG
ApaI 77 GGGCCC GGGCC^C
88 GGGCCC
BalI 714 TGGCCA TGG^CCA
806 TGGCCA
BciVI 1270 GTATCC GTATCC(6/5)
1466 GTATCC
BfaI 128 CTAG C^TAG
952 CTAG
BspMI 461 ACCTGC ACCTGC(4/8)
1120 GCAGGT
Bsu36I 1132 CCTGAGG CC^T[ACGT]AGG
1279 CCTCAGG
Eco81I 1132 CCTGAGG CC^T[ACGT]AGG
1279 CCTCAGG
EcoNI 216 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1275 CCTCCCTCAGG
EcoT14I 1397 CCAAGG C^C[AT][AT]GG
1512 CCATGG
MaeI 128 CTAG C^TAG
952 CTAG
MluNI 714 TGGCCA TGG^CCA
806 TGGCCA
MscI 714 TGGCCA TGG^CCA
806 TGGCCA
NaeI 533 GCCGGC GCC^GGC
1322 GCCGGC
NgoMIV 533 GCCGGC G^CCGGC
1322 GCCGGC
StyI 1397 CCAAGG C^C[AT][AT]GG
1512 CCATGG
TaqI 797 TCGA T^CGA
1092 TCGA
Tsp509I 1027 AATT ^AATT
1574 AATT
TthHB8I 797 TCGA T^CGA
1092 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI Asp700I AspHI
AvaI AvaII BanI BanII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
XcmI XmnI