Restriction map for
pF1KA1732
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# NO CUT ENZYMES ( 47 enzymes) #
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AatII Acc65I AflII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AviII
AvrII BcgI BciVI BfrI BglI
BlnI BsiWI BssHII Bst1107I BstZ17I
CpoI DraIII Eco47III FseI FspI
KpnI KspI MluI NheI NotI
NruI PacI PinAI PmeI PvuI
RsrII SacII SalI SbfI SfiI
SgrAI SmaI SnaBI Sse8387I SwaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 3420 TCCGGA T^CCGGA
AclI 5220 AACGTT AA^CGTT
AcyI 4961 GGCGCC G[GA]^CG[CT]C
AhdI 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4336 GGGCCC GGGCC^C
AspEI 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4839 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4961 GGCGCC GGCGC^C
BbrPI 3887 CACGTG CAC^GTG
BsaHI 4961 GGCGCC G[GA]^CG[CT]C
BseAI 3420 TCCGGA T^CCGGA
BsiEI 5598 CGGCCG CG[GA][CT]^CG
BspDI 1856 ATCGAT AT^CGAT
BspEI 3420 TCCGGA T^CCGGA
BsrBI 6499 GAGCGG CCGCTC(-3/-3)
BsrFI 7011 GCCGGC [GA]^CCGG[CT]
BssSI 1364 CACGAG CACGAG(-5/-1)
BstBI 4713 TTCGAA TT^CGAA
Cfr10I 7011 GCCGGC [GA]^CCGG[CT]
ClaI 1856 ATCGAT AT^CGAT
DrdI 7130 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5598 CGGCCG C^GGCCG
Eam1105I 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5598 CGGCCG C^GGCCG
Eco52I 5598 CGGCCG C^GGCCG
EcoRV 4189 GATATC GAT^ATC
HaeII 4961 GGCGCC [GA]GCGC^[CT]
Hin1I 4961 GGCGCC G[GA]^CG[CT]C
HincII 5217 GTTAAC GT[CT]^[GA]AC
HindII 5217 GTTAAC GT[CT]^[GA]AC
HindIII 5617 AAGCTT A^AGCTT
HpaI 5217 GTTAAC GTT^AAC
KasI 4961 GGCGCC G^GCGCC
MroI 3420 TCCGGA T^CCGGA
NaeI 7011 GCCGGC GCC^GGC
NarI 4961 GGCGCC GG^CGCC
NdeI 7562 CATATG CA^TATG
NgoMIV 7011 GCCGGC G^CCGGC
NspV 4713 TTCGAA TT^CGAA
PflFI 4839 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6948 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3887 CACGTG CAC^GTG
PmlI 3887 CACGTG CAC^GTG
Psp1406I 5220 AACGTT AA^CGTT
SfoI 4961 GGCGCC GGC^GCC
SfuI 4713 TTCGAA TT^CGAA
SpeI 2575 ACTAGT A^CTAGT
Tth111I 4839 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6948 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1237 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccII 1331 CGCG CG^CG
5780 CGCG
AocI 1446 CCTAAGG CC^T[ACGT]AGG
7813 CCTGAGG
AseI 1807 ATTAAT AT^TAAT
6154 ATTAAT
AsnI 1807 ATTAAT AT^TAAT
6154 ATTAAT
Asp700I 868 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5262 GAAATGTTTC
AvaI 4772 CTCGAG C^[CT]CG[GA]G
7427 CTCGAG
BalI 5023 TGGCCA TGG^CCA
6257 TGGCCA
BbvCI 4932 CCTCAGC CCTCAGC(-5/-2)
7315 CCTCAGC
BsaWI 3420 TCCGGA [AT]^CCGG[AT]
7700 ACCGGA
BsmI 4487 GAATGC GAATGC(1/-1)
6037 GAATGC
BsoBI 4772 CTCGAG C^[CT]CG[GA]G
7427 CTCGAG
BstAPI 4896 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6648 GCAACAGATGC
BstUI 1331 CGCG CG^CG
5780 CGCG
Bsu36I 1446 CCTAAGG CC^T[ACGT]AGG
7813 CCTGAGG
CfoI 1332 GCGC GCG^C
4962 GCGC
DsaI 3023 CCATGG C^C[GA][CT]GG
3236 CCATGG
Eco81I 1446 CCTAAGG CC^T[ACGT]AGG
7813 CCTGAGG
EcoT22I 2139 ATGCAT ATGCA^T
3050 ATGCAT
HgaI 1329 GACGC GACGC(5/10)
6103 GACGC
HhaI 1332 GCGC GCG^C
4962 GCGC
HinP1I 1332 GCGC G^CGC
4962 GCGC
MfeI 5283 CAATTG C^AATTG
7187 CAATTG
MluNI 5023 TGGCCA TGG^CCA
6257 TGGCCA
MscI 5023 TGGCCA TGG^CCA
6257 TGGCCA
MunI 5283 CAATTG C^AATTG
7187 CAATTG
MvnI 1331 CGCG CG^CG
5780 CGCG
NcoI 3023 CCATGG C^CATGG
3236 CCATGG
NsiI 2139 ATGCAT ATGCA^T
3050 ATGCAT
PaeR7I 4772 CTCGAG C^TCGAG
7427 CTCGAG
PpuMI 4364 GGGACCC [GA]G^G[AT]CC[CT]
7817 AGGACCT
PshAI 1619 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5727 GACTGCTGTC
PshBI 1807 ATTAAT AT^TAAT
6154 ATTAAT
PstI 10 CTGCAG CTGCA^G
377 CTGCAG
SexAI 5432 ACCAGGT A^CC[AT]GGT
6738 ACCAGGT
StuI 6755 AGGCCT AGG^CCT
7583 AGGCCT
XhoI 4772 CTCGAG C^TCGAG
7427 CTCGAG
XmnI 868 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5262 GAAATGTTTC
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# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BamHI BanI BanII BbsI
BbvI BclI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII