################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII Acc65I AflII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AviII AvrII BcgI BciVI BfrI BglI BlnI BsiWI BssHII Bst1107I BstZ17I CpoI DraIII Eco47III FseI FspI KpnI KspI MluI NheI NotI NruI PacI PinAI PmeI PvuI RsrII SacII SalI SbfI SfiI SgrAI SmaI SnaBI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 3420 TCCGGA T^CCGGA AclI 5220 AACGTT AA^CGTT AcyI 4961 GGCGCC G[GA]^CG[CT]C AhdI 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 4336 GGGCCC GGGCC^C AspEI 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4839 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 4961 GGCGCC GGCGC^C BbrPI 3887 CACGTG CAC^GTG BsaHI 4961 GGCGCC G[GA]^CG[CT]C BseAI 3420 TCCGGA T^CCGGA BsiEI 5598 CGGCCG CG[GA][CT]^CG BspDI 1856 ATCGAT AT^CGAT BspEI 3420 TCCGGA T^CCGGA BsrBI 6499 GAGCGG CCGCTC(-3/-3) BsrFI 7011 GCCGGC [GA]^CCGG[CT] BssSI 1364 CACGAG CACGAG(-5/-1) BstBI 4713 TTCGAA TT^CGAA Cfr10I 7011 GCCGGC [GA]^CCGG[CT] ClaI 1856 ATCGAT AT^CGAT DrdI 7130 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 5598 CGGCCG C^GGCCG Eam1105I 4087 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 5598 CGGCCG C^GGCCG Eco52I 5598 CGGCCG C^GGCCG EcoRV 4189 GATATC GAT^ATC HaeII 4961 GGCGCC [GA]GCGC^[CT] Hin1I 4961 GGCGCC G[GA]^CG[CT]C HincII 5217 GTTAAC GT[CT]^[GA]AC HindII 5217 GTTAAC GT[CT]^[GA]AC HindIII 5617 AAGCTT A^AGCTT HpaI 5217 GTTAAC GTT^AAC KasI 4961 GGCGCC G^GCGCC MroI 3420 TCCGGA T^CCGGA NaeI 7011 GCCGGC GCC^GGC NarI 4961 GGCGCC GG^CGCC NdeI 7562 CATATG CA^TATG NgoMIV 7011 GCCGGC G^CCGGC NspV 4713 TTCGAA TT^CGAA PflFI 4839 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 6948 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3887 CACGTG CAC^GTG PmlI 3887 CACGTG CAC^GTG Psp1406I 5220 AACGTT AA^CGTT SfoI 4961 GGCGCC GGC^GCC SfuI 4713 TTCGAA TT^CGAA SpeI 2575 ACTAGT A^CTAGT Tth111I 4839 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 6948 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1237 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 1331 CGCG CG^CG 5780 CGCG AocI 1446 CCTAAGG CC^T[ACGT]AGG 7813 CCTGAGG AseI 1807 ATTAAT AT^TAAT 6154 ATTAAT AsnI 1807 ATTAAT AT^TAAT 6154 ATTAAT Asp700I 868 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5262 GAAATGTTTC AvaI 4772 CTCGAG C^[CT]CG[GA]G 7427 CTCGAG BalI 5023 TGGCCA TGG^CCA 6257 TGGCCA BbvCI 4932 CCTCAGC CCTCAGC(-5/-2) 7315 CCTCAGC BsaWI 3420 TCCGGA [AT]^CCGG[AT] 7700 ACCGGA BsmI 4487 GAATGC GAATGC(1/-1) 6037 GAATGC BsoBI 4772 CTCGAG C^[CT]CG[GA]G 7427 CTCGAG BstAPI 4896 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6648 GCAACAGATGC BstUI 1331 CGCG CG^CG 5780 CGCG Bsu36I 1446 CCTAAGG CC^T[ACGT]AGG 7813 CCTGAGG CfoI 1332 GCGC GCG^C 4962 GCGC DsaI 3023 CCATGG C^C[GA][CT]GG 3236 CCATGG Eco81I 1446 CCTAAGG CC^T[ACGT]AGG 7813 CCTGAGG EcoT22I 2139 ATGCAT ATGCA^T 3050 ATGCAT HgaI 1329 GACGC GACGC(5/10) 6103 GACGC HhaI 1332 GCGC GCG^C 4962 GCGC HinP1I 1332 GCGC G^CGC 4962 GCGC MfeI 5283 CAATTG C^AATTG 7187 CAATTG MluNI 5023 TGGCCA TGG^CCA 6257 TGGCCA MscI 5023 TGGCCA TGG^CCA 6257 TGGCCA MunI 5283 CAATTG C^AATTG 7187 CAATTG MvnI 1331 CGCG CG^CG 5780 CGCG NcoI 3023 CCATGG C^CATGG 3236 CCATGG NsiI 2139 ATGCAT ATGCA^T 3050 ATGCAT PaeR7I 4772 CTCGAG C^TCGAG 7427 CTCGAG PpuMI 4364 GGGACCC [GA]G^G[AT]CC[CT] 7817 AGGACCT PshAI 1619 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5727 GACTGCTGTC PshBI 1807 ATTAAT AT^TAAT 6154 ATTAAT PstI 10 CTGCAG CTGCA^G 377 CTGCAG SexAI 5432 ACCAGGT A^CC[AT]GGT 6738 ACCAGGT StuI 6755 AGGCCT AGG^CCT 7583 AGGCCT XhoI 4772 CTCGAG C^TCGAG 7427 CTCGAG XmnI 868 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5262 GAAATGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BamHI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII