Restriction map for pF1KA1732
##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        Asp718I     AviII       
AvrII       BcgI        BciVI       BfrI        BglI        
BlnI        BsiWI       BssHII      Bst1107I    BstZ17I     
CpoI        DraIII      Eco47III    FseI        FspI        
KpnI        KspI        MluI        NheI        NotI        
NruI        PacI        PinAI       PmeI        PvuI        
RsrII       SacII       SalI        SbfI        SfiI        
SgrAI       SmaI        SnaBI       Sse8387I    SwaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       3420  TCCGGA               T^CCGGA
AclI         5220  AACGTT               AA^CGTT
AcyI         4961  GGCGCC               G[GA]^CG[CT]C
AhdI         4087  GACAAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         4336  GGGCCC               GGGCC^C
AspEI        4087  GACAAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4839  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         4961  GGCGCC               GGCGC^C
BbrPI        3887  CACGTG               CAC^GTG
BsaHI        4961  GGCGCC               G[GA]^CG[CT]C
BseAI        3420  TCCGGA               T^CCGGA
BsiEI        5598  CGGCCG               CG[GA][CT]^CG
BspDI        1856  ATCGAT               AT^CGAT
BspEI        3420  TCCGGA               T^CCGGA
BsrBI        6499  GAGCGG               CCGCTC(-3/-3)
BsrFI        7011  GCCGGC               [GA]^CCGG[CT]
BssSI        1364  CACGAG               CACGAG(-5/-1)
BstBI        4713  TTCGAA               TT^CGAA
Cfr10I       7011  GCCGGC               [GA]^CCGG[CT]
ClaI         1856  ATCGAT               AT^CGAT
DrdI         7130  GACAGACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         5598  CGGCCG               C^GGCCG
Eam1105I     4087  GACAAGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5598  CGGCCG               C^GGCCG
Eco52I       5598  CGGCCG               C^GGCCG
EcoRV        4189  GATATC               GAT^ATC
HaeII        4961  GGCGCC               [GA]GCGC^[CT]
Hin1I        4961  GGCGCC               G[GA]^CG[CT]C
HincII       5217  GTTAAC               GT[CT]^[GA]AC
HindII       5217  GTTAAC               GT[CT]^[GA]AC
HindIII      5617  AAGCTT               A^AGCTT
HpaI         5217  GTTAAC               GTT^AAC
KasI         4961  GGCGCC               G^GCGCC
MroI         3420  TCCGGA               T^CCGGA
NaeI         7011  GCCGGC               GCC^GGC
NarI         4961  GGCGCC               GG^CGCC
NdeI         7562  CATATG               CA^TATG
NgoMIV       7011  GCCGGC               G^CCGGC
NspV         4713  TTCGAA               TT^CGAA
PflFI        4839  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        6948  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3887  CACGTG               CAC^GTG
PmlI         3887  CACGTG               CAC^GTG
Psp1406I     5220  AACGTT               AA^CGTT
SfoI         4961  GGCGCC               GGC^GCC
SfuI         4713  TTCGAA               TT^CGAA
SpeI         2575  ACTAGT               A^CTAGT
Tth111I      4839  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       6948  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1237  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccII        1331  CGCG                 CG^CG
             5780  CGCG                
AocI         1446  CCTAAGG              CC^T[ACGT]AGG
             7813  CCTGAGG             
AseI         1807  ATTAAT               AT^TAAT
             6154  ATTAAT              
AsnI         1807  ATTAAT               AT^TAAT
             6154  ATTAAT              
Asp700I       868  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5262  GAAATGTTTC          
AvaI         4772  CTCGAG               C^[CT]CG[GA]G
             7427  CTCGAG              
BalI         5023  TGGCCA               TGG^CCA
             6257  TGGCCA              
BbvCI        4932  CCTCAGC              CCTCAGC(-5/-2)
             7315  CCTCAGC             
BsaWI        3420  TCCGGA               [AT]^CCGG[AT]
             7700  ACCGGA              
BsmI         4487  GAATGC               GAATGC(1/-1)
             6037  GAATGC              
BsoBI        4772  CTCGAG               C^[CT]CG[GA]G
             7427  CTCGAG              
BstAPI       4896  GCAACCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6648  GCAACAGATGC         
BstUI        1331  CGCG                 CG^CG
             5780  CGCG                
Bsu36I       1446  CCTAAGG              CC^T[ACGT]AGG
             7813  CCTGAGG             
CfoI         1332  GCGC                 GCG^C
             4962  GCGC                
DsaI         3023  CCATGG               C^C[GA][CT]GG
             3236  CCATGG              
Eco81I       1446  CCTAAGG              CC^T[ACGT]AGG
             7813  CCTGAGG             
EcoT22I      2139  ATGCAT               ATGCA^T
             3050  ATGCAT              
HgaI         1329  GACGC                GACGC(5/10)
             6103  GACGC               
HhaI         1332  GCGC                 GCG^C
             4962  GCGC                
HinP1I       1332  GCGC                 G^CGC
             4962  GCGC                
MfeI         5283  CAATTG               C^AATTG
             7187  CAATTG              
MluNI        5023  TGGCCA               TGG^CCA
             6257  TGGCCA              
MscI         5023  TGGCCA               TGG^CCA
             6257  TGGCCA              
MunI         5283  CAATTG               C^AATTG
             7187  CAATTG              
MvnI         1331  CGCG                 CG^CG
             5780  CGCG                
NcoI         3023  CCATGG               C^CATGG
             3236  CCATGG              
NsiI         2139  ATGCAT               ATGCA^T
             3050  ATGCAT              
PaeR7I       4772  CTCGAG               C^TCGAG
             7427  CTCGAG              
PpuMI        4364  GGGACCC              [GA]G^G[AT]CC[CT]
             7817  AGGACCT             
PshAI        1619  GACGAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5727  GACTGCTGTC          
PshBI        1807  ATTAAT               AT^TAAT
             6154  ATTAAT              
PstI           10  CTGCAG               CTGCA^G
              377  CTGCAG              
SexAI        5432  ACCAGGT              A^CC[AT]GGT
             6738  ACCAGGT             
StuI         6755  AGGCCT               AGG^CCT
             7583  AGGCCT              
XhoI         4772  CTCGAG               C^TCGAG
             7427  CTCGAG              
XmnI          868  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5262  GAAATGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BamHI       BanI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       Bsp1286I    Bsp1407I    BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstXI       BstYI       Cac8I       CelII       Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SphI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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