Restriction map for
pF1KA1624
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AviII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII MluI MluNI MroI MscI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SapI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 415 GGTACC G^GTACC
AccI 1219 GTCGAC GT^[AC][GT]AC
AlwI 1526 GGATC GGATC(4/5)
Asp700I 1473 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 415 GGTACC G^GTACC
AvaI 1379 CCCGGG C^[CT]CG[GA]G
AvrII 43 CCTAGG C^CTAGG
BanII 1771 GAGCCC G[GA]GC[CT]^C
BglI 4 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1103 AGATCT A^GATCT
BlnI 43 CCTAGG C^CTAGG
BsaBI 840 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1379 CCCGGG C^[CT]CG[GA]G
BspHI 1109 TCATGA T^CATGA
BsrBI 5 CCGCTC CCGCTC(-3/-3)
BstBI 851 TTCGAA TT^CGAA
BstEII 1752 GGTGACC G^GT[ACGT]ACC
BstPI 1752 GGTGACC G^GT[ACGT]ACC
BstYI 1103 AGATCT [GA]^GATC[CT]
EcoNI 973 CCTATCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1752 GGTGACC G^GT[ACGT]ACC
HgaI 119 GCGTC GACGC(5/10)
HpaI 484 GTTAAC GTT^AAC
KpnI 415 GGTACC GGTAC^C
MaeII 1493 ACGT A^CGT
MamI 840 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 938 CAATTG C^AATTG
MflI 1103 AGATCT [GA]^GATC[CT]
MunI 938 CAATTG C^AATTG
NcoI 566 CCATGG C^CATGG
NspV 851 TTCGAA TT^CGAA
PstI 88 CTGCAG CTGCA^G
PvuII 1448 CAGCTG CAG^CTG
RcaI 1109 TCATGA T^CATGA
SalI 1219 GTCGAC G^TCGAC
SfaNI 1285 GCATC GCATC(5/9)
SfcI 88 CTGCAG C^T[GA][CT]AG
SfuI 851 TTCGAA TT^CGAA
SmaI 1379 CCCGGG CCC^GGG
SmlI 136 CTCAAG C^T[CT][GA]AG
StuI 654 AGGCCT AGG^CCT
XcmI 1757 CCAACGGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1103 AGATCT [GA]^GATC[CT]
XmaCI 1379 CCCGGG C^CCGGG
XmaI 1379 CCCGGG C^CCGGG
XmnI 1473 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 776 AAATTC [GA]^AATT[CT]
1633 AAATTC
AcyI 12 GGCGCC G[GA]^CG[CT]C
1792 GGCGCC
ApoI 776 AAATTC [GA]^AATT[CT]
1633 AAATTC
BbeI 12 GGCGCC GGCGC^C
1792 GGCGCC
BcnI 1379 CCCGG CC^[GC]GG
1380 CCGGG
BmyI 1176 GGGCAC G[AGT]GC[ACT]^C
1771 GAGCCC
BpmI 375 CTCCAG CTGGAG(16/14)
1126 CTCCAG
BsaHI 12 GGCGCC G[GA]^CG[CT]C
1792 GGCGCC
BsmFI 829 GGGAC GGGAC(10/14)
1317 GGGAC
Bsp1286I 1176 GGGCAC G[AGT]GC[ACT]^C
1771 GAGCCC
BsrDI 1041 CATTGC GCAATG(2/0)
1530 CATTGC
DraI 330 TTTAAA TTT^AAA
874 TTTAAA
DraII 1130 AGGTCCT [GA]G^G[ACGT]CC[CT]
1317 GGGACCT
EcoO109I 1130 AGGTCCT [GA]G^G[ACGT]CC[CT]
1317 GGGACCT
Hin1I 12 GGCGCC G[GA]^CG[CT]C
1792 GGCGCC
HincII 484 GTTAAC GT[CT]^[GA]AC
1219 GTCGAC
HindII 484 GTTAAC GT[CT]^[GA]AC
1219 GTCGAC
KasI 12 GGCGCC G^GCGCC
1792 GGCGCC
KspI 36 CCGCGG CCGC^GG
53 CCGCGG
NarI 12 GGCGCC GG^CGCC
1792 GGCGCC
NciI 1379 CCCGG CC^[GC]GG
1380 CCGGG
PpuMI 1130 AGGTCCT [GA]G^G[AT]CC[CT]
1317 GGGACCT
SacII 36 CCGCGG CCGC^GG
53 CCGCGG
SfoI 12 GGCGCC GGC^GCC
1792 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanI BbsI BbvI BfaI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I