Restriction map for
pF1KA1604
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BanII BbeI BbrPI
BcgI BciVI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 1929 GCGG CCGC(-3/-1)
AflII 889 CTTAAG C^TTAAG
AspHI 1415 GTGCTC G[AT]GC[AT]^C
BbvCI 2135 CCTCAGC CCTCAGC(-5/-2)
BfrI 889 CTTAAG C^TTAAG
BlpI 452 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 452 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 1415 GTGCTC G[AT]GC[AT]^C
BsmBI 2628 GAGACG CGTCTC(1/5)
BsmI 162 GCATTC GAATGC(1/-1)
BspMI 336 ACCTGC ACCTGC(4/8)
BsrFI 2690 ACCGGC [GA]^CCGG[CT]
BstAPI 610 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1248 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 452 GCTTAGC GC^T[ACGT]AGC
Cfr10I 2690 ACCGGC [GA]^CCGG[CT]
DraII 479 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 611 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1681 CCATGG C^C[GA][CT]GG
EcoO109I 479 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 2609 GATATC GAT^ATC
EcoT14I 1681 CCATGG C^C[AT][AT]GG
EcoT22I 2422 ATGCAT ATGCA^T
FauI 1929 GCGGG CCCGC(4/6)
HgaI 2020 GCGTC GACGC(5/10)
HincII 517 GTCAAC GT[CT]^[GA]AC
HindII 517 GTCAAC GT[CT]^[GA]AC
MaeII 177 ACGT A^CGT
NcoI 1681 CCATGG C^CATGG
NdeI 1102 CATATG CA^TATG
NsiI 2422 ATGCAT ATGCA^T
NspI 1544 GCATGC [GA]CATG^[CT]
PshAI 584 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1822 CTGCAG CTGCA^G
SexAI 1352 ACCTGGT A^CC[AT]GGT
SmaI 119 CCCGGG CCC^GGG
SphI 1544 GCATGC GCATG^C
SspI 1752 AATATT AAT^ATT
StyI 1681 CCATGG C^C[AT][AT]GG
XcmI 1604 CCATCCATCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 119 CCCGGG C^CCGGG
XmaI 119 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 1762 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2033 CAGAGTCTG
AseI 496 ATTAAT AT^TAAT
693 ATTAAT
AsnI 496 ATTAAT AT^TAAT
693 ATTAAT
AvaI 119 CCCGGG C^[CT]CG[GA]G
1850 CCCGAG
BamHI 366 GGATCC G^GATCC
1161 GGATCC
BanI 2255 GGCACC G^G[CT][GA]CC
2620 GGTGCC
BbvI 646 GCAGC GCAGC(8/12)
1824 GCAGC
BclI 1089 TGATCA T^GATCA
2198 TGATCA
BcnI 119 CCCGG CC^[GC]GG
120 CCGGG
BfaI 1060 CTAG C^TAG
2645 CTAG
BsaBI 244 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2605 GATAGATATC
BsmFI 212 GGGAC GGGAC(10/14)
2160 GGGAC
BsoBI 119 CCCGGG C^[CT]CG[GA]G
1850 CCCGAG
BspHI 39 TCATGA T^CATGA
1332 TCATGA
BstBI 722 TTCGAA TT^CGAA
956 TTCGAA
Cac8I 606 GCAAGC GC[ACGT]^[ACGT]GC
1544 GCATGC
CfoI 781 GCGC GCG^C
1966 GCGC
FbaI 1089 TGATCA T^GATCA
2198 TGATCA
Fnu4HI 646 GCAGC GC^[ACGT]GC
1824 GCAGC
HapII 120 CCGG C^CGG
2691 CCGG
HhaI 781 GCGC GCG^C
1966 GCGC
HinP1I 781 GCGC G^CGC
1966 GCGC
HpaII 120 CCGG C^CGG
2691 CCGG
ItaI 646 GCAGC GC^[ACGT]GC
1824 GCAGC
MaeI 1060 CTAG C^TAG
2645 CTAG
MamI 244 GATACGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2605 GATAGATATC
MspI 120 CCGG C^CGG
2691 CCGG
NciI 119 CCCGG CC^[GC]GG
120 CCGGG
NspV 722 TTCGAA TT^CGAA
956 TTCGAA
PshBI 496 ATTAAT AT^TAAT
693 ATTAAT
RcaI 39 TCATGA T^CATGA
1332 TCATGA
SfuI 722 TTCGAA TT^CGAA
956 TTCGAA
SmlI 889 CTTAAG C^T[CT][GA]AG
2589 CTCAAG
TseI 646 GCAGC G^C[AT]GC
1824 GCAGC
Tsp45I 201 GTCAC ^GT[GC]AC
921 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BbsI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII FokI HaeIII
HinfI HphI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI