Restriction map for
pF1KA1582
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# NO CUT ENZYMES ( 54 enzymes) #
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AccI AflII AhdI AscI AspEI
AviII BbeI BciVI BfrI BsaBI
BsiEI BsiWI Bsp1407I BsrBI BsrGI
Bst1107I BstZ17I CpoI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FseI
FspI HindIII KasI MamI MfeI
MluI MunI NarI NotI NruI
NsiI PacI PaeR7I PmeI PshAI
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfoI SnaBI SpeI
Sse8387I SspBI SwaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 3532 TCCGGA T^CCGGA
AclI 3485 AACGTT AA^CGTT
Alw44I 3137 GTGCAC G^TGCAC
Aor51HI 2917 AGCGCT AGC^GCT
ApaI 4977 GGGCCC GGGCC^C
ApaLI 3137 GTGCAC G^TGCAC
Asp700I 4767 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4329 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1396 CCTAGG C^CTAGG
BbrPI 4103 CACGTG CAC^GTG
BglII 1903 AGATCT A^GATCT
BlnI 1396 CCTAGG C^CTAGG
BsaAI 4103 CACGTG [CT]AC^GT[GA]
BsaI 3397 GGTCTC GGTCTC(1/5)
BseAI 3532 TCCGGA T^CCGGA
BsmBI 2326 GAGACG CGTCTC(1/5)
BsmI 1047 GCATTC GAATGC(1/-1)
BspDI 2363 ATCGAT AT^CGAT
BspEI 3532 TCCGGA T^CCGGA
BssHII 3565 GCGCGC G^CGCGC
BstBI 4553 TTCGAA TT^CGAA
BstEII 760 GGTAACC G^GT[ACGT]ACC
BstPI 760 GGTAACC G^GT[ACGT]ACC
ClaI 2363 ATCGAT AT^CGAT
DraI 1974 TTTAAA TTT^AAA
DraIII 3131 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4216 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2917 AGCGCT AGC^GCT
EcoO65I 760 GGTAACC G^GT[ACGT]ACC
HpaI 4431 GTTAAC GTT^AAC
KspI 4840 CCGCGG CCGC^GG
MroI 3532 TCCGGA T^CCGGA
NaeI 2822 GCCGGC GCC^GGC
NdeI 3439 CATATG CA^TATG
NgoMIV 2822 GCCGGC G^CCGGC
NheI 2002 GCTAGC G^CTAGC
NspV 4553 TTCGAA TT^CGAA
PflFI 4329 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4103 CACGTG CAC^GTG
PmlI 4103 CACGTG CAC^GTG
Psp1406I 3485 AACGTT AA^CGTT
SacII 4840 CCGCGG CCGC^GG
SfuI 4553 TTCGAA TT^CGAA
SgrAI 2552 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1599 CCCGGG CCC^GGG
SphI 3182 GCATGC GCATG^C
SspI 4198 AATATT AAT^ATT
Tth111I 4329 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3202 TCTAGA T^CTAGA
XmaCI 1599 CCCGGG C^CCGGG
XmaI 1599 CCCGGG C^CCGGG
XmnI 4767 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AatII 2787 GACGTC GACGT^C
4222 GACGTC
Acc65I 2088 GGTACC G^GTACC
2179 GGTACC
AgeI 2091 ACCGGT A^CCGGT
3844 ACCGGT
AocI 3352 CCTCAGG CC^T[ACGT]AGG
4523 CCTCAGG
AseI 286 ATTAAT AT^TAAT
3427 ATTAAT
AsnI 286 ATTAAT AT^TAAT
3427 ATTAAT
Asp718I 2088 GGTACC G^GTACC
2179 GGTACC
BalI 1662 TGGCCA TGG^CCA
4890 TGGCCA
BamHI 847 GGATCC G^GATCC
3535 GGATCC
BanII 3079 GAGCCC G[GA]GC[CT]^C
4977 GGGCCC
BclI 2828 TGATCA T^GATCA
2904 TGATCA
BlpI 4845 GCTCAGC GC^T[ACGT]AGC
5052 GCTCAGC
Bpu1102I 4845 GCTCAGC GC^T[ACGT]AGC
5052 GCTCAGC
BspLU11I 2228 ACATGT A^CATGT
4709 ACATGT
BsrDI 595 GCAATG GCAATG(2/0)
4649 GCAATG
BstAPI 826 GCATTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2578 GCACCTGTTGC
Bsu36I 3352 CCTCAGG CC^T[ACGT]AGG
4523 CCTCAGG
CelII 4845 GCTCAGC GC^T[ACGT]AGC
5052 GCTCAGC
Eco81I 3352 CCTCAGG CC^T[ACGT]AGG
4523 CCTCAGG
EcoNI 766 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
929 CCTGGGGAAGG
EcoRI 3195 GAATTC G^AATTC
4162 GAATTC
FbaI 2828 TGATCA T^GATCA
2904 TGATCA
KpnI 2088 GGTACC GGTAC^C
2179 GGTACC
MluNI 1662 TGGCCA TGG^CCA
4890 TGGCCA
MscI 1662 TGGCCA TGG^CCA
4890 TGGCCA
MslI 2841 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3834 CATGGACATG
PinAI 2091 ACCGGT A^CCGGT
3844 ACCGGT
PshBI 286 ATTAAT AT^TAAT
3427 ATTAAT
SapI 2567 GAAGAGC GCTCTTC(1/4)
3076 GAAGAGC
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII